Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   THIMO_RS04990 Genome accession   NC_019940
Coordinates   1131959..1133674 (-) Length   571 a.a.
NCBI ID   WP_015280000.1    Uniprot ID   L0GWW0
Organism   Thioflavicoccus mobilis 8321     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1126959..1138674
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  THIMO_RS04965 (Thimo_1045) ubiA 1127390..1128295 (+) 906 WP_015279995.1 4-hydroxybenzoate octaprenyltransferase -
  THIMO_RS04970 (Thimo_1046) - 1128532..1128804 (-) 273 WP_015279996.1 hypothetical protein -
  THIMO_RS04975 (Thimo_1047) coaE 1128976..1129590 (-) 615 WP_015279997.1 dephospho-CoA kinase -
  THIMO_RS04980 (Thimo_1048) pilD 1129587..1130465 (-) 879 WP_015279998.1 A24 family peptidase Machinery gene
  THIMO_RS04985 (Thimo_1049) pilC 1130700..1131953 (-) 1254 WP_015279999.1 type II secretion system F family protein Machinery gene
  THIMO_RS04990 (Thimo_1050) pilB 1131959..1133674 (-) 1716 WP_015280000.1 type IV-A pilus assembly ATPase PilB Machinery gene
  THIMO_RS04995 (Thimo_1051) - 1134321..1135154 (-) 834 WP_015280001.1 NYN domain-containing protein -
  THIMO_RS05000 (Thimo_1052) acs 1135404..1137350 (-) 1947 WP_015280002.1 acetate--CoA ligase -

Sequence

Protein


Download         Length: 571 a.a.        Molecular weight: 63469.75 Da        Isoelectric Point: 5.6096

>NTDB_id=55434 THIMO_RS04990 WP_015280000.1 1131959..1133674(-) (pilB) [Thioflavicoccus mobilis 8321]
MATTQPSLNPSGLAWRLVRDRLITEDVARTAHAEASKRREPFVHYLVQQQILDSRRIAIAACHEFGVPLFDISALDLQQA
PVNLVDERLIRRHRALPIFRRGNRLFLAVSDPTNDQGVDEIRFNTGLTIEMVLAEEDKLSAAIDRAIEAQDTTTMTEIMD
AELETLDIASDDDKSNRDNDLNIDEAPIVRYVNKILIDAITSGASDVHFEPYEKTFRIRFRQDGLLREVASPPSNLAARL
VARLKVMSRMNIAERRIPQDGRIKLKLSRNRDIDFRVNTLPTLYGEKVVLRILDSSASTVDVEDLGMDPEQRDVFLEAID
KPYGMVLVTGPTGSGKTVSLYSALNLLNKPEVNISTVEDPVEIVVPGINQVNMNPKTGLTFAAALRAFLRQDPDIIMVGE
VRDLETAEIAVKAAQTGHMVLSTLHTNDAPQSLTRLANMGVAPFNIASSILLIMAQRLVRRLCTHCRKPEEIPRETLLVE
GFTEKEIEEGLTIYGPVGCERCTKGYKGRTGIFQVMKVSDAISRLILEGGNALQLAEQAKHEGVADLRRSGLSKVKAGIT
SLEELNRVTKE

Nucleotide


Download         Length: 1716 bp        

>NTDB_id=55434 THIMO_RS04990 WP_015280000.1 1131959..1133674(-) (pilB) [Thioflavicoccus mobilis 8321]
ATGGCAACCACGCAACCTTCCCTGAACCCTTCCGGCCTCGCCTGGCGGCTGGTGCGCGATCGCCTGATCACTGAGGACGT
GGCCCGCACCGCGCACGCCGAAGCCTCGAAGCGCCGCGAGCCGTTCGTGCACTACCTGGTGCAGCAACAGATCCTCGACA
GCCGGCGCATCGCCATCGCTGCCTGCCACGAATTCGGGGTGCCACTGTTCGACATCAGCGCCCTCGATCTGCAACAGGCG
CCTGTCAATCTGGTCGATGAGCGCTTGATCCGCCGTCATCGCGCGCTACCCATCTTTCGTCGTGGCAATCGGCTCTTCCT
CGCCGTCTCCGATCCGACCAATGATCAGGGCGTCGACGAGATCCGCTTCAACACGGGCCTGACGATCGAGATGGTCCTGG
CCGAGGAGGACAAGCTTAGCGCCGCCATCGATCGCGCGATCGAGGCGCAAGACACGACGACGATGACGGAGATCATGGAT
GCCGAGCTGGAGACGCTCGACATCGCCTCCGACGACGACAAGAGCAACCGCGACAACGATCTCAACATCGACGAAGCCCC
GATCGTCCGCTATGTCAACAAGATCCTCATCGACGCGATCACCAGCGGGGCCTCCGACGTCCATTTCGAACCCTACGAGA
AGACCTTCCGGATCCGCTTCCGCCAGGACGGCCTGCTGCGCGAGGTCGCGAGCCCACCGAGCAATCTCGCCGCCCGCCTA
GTGGCGCGCCTGAAGGTCATGTCGCGGATGAATATCGCCGAGCGACGCATCCCCCAGGATGGGCGAATCAAGCTCAAGCT
CAGCCGCAACCGCGATATCGACTTCCGCGTCAACACGCTACCGACCCTCTACGGTGAGAAGGTCGTGCTGCGCATCCTGG
ACTCCTCGGCCTCGACGGTCGACGTCGAGGATCTCGGCATGGACCCAGAGCAACGCGATGTCTTTCTCGAGGCGATCGAC
AAGCCCTACGGAATGGTCCTCGTCACGGGGCCGACAGGTTCGGGCAAGACGGTCTCTCTCTACTCGGCGCTCAACCTGCT
GAACAAACCGGAGGTCAACATCTCGACGGTCGAGGACCCGGTCGAGATCGTGGTGCCGGGCATCAACCAAGTCAACATGA
ACCCGAAGACTGGCTTGACCTTCGCCGCGGCGCTGCGCGCCTTCCTGCGCCAGGACCCGGACATCATCATGGTCGGCGAG
GTGCGCGACCTCGAAACCGCCGAGATCGCCGTCAAGGCCGCCCAAACCGGCCACATGGTCTTGTCCACACTCCACACCAA
CGACGCACCGCAGTCGCTGACCCGACTGGCCAACATGGGCGTCGCCCCATTCAACATCGCCTCGTCCATCCTGTTGATCA
TGGCCCAACGTCTGGTGCGCCGCCTCTGCACGCACTGCCGGAAACCGGAGGAGATTCCGCGCGAGACGCTCTTGGTCGAA
GGCTTCACCGAGAAGGAGATCGAGGAAGGGCTGACCATTTATGGGCCGGTCGGCTGCGAGCGCTGTACCAAAGGCTATAA
AGGCCGTACCGGTATTTTCCAGGTCATGAAGGTCTCCGATGCGATCAGCCGTCTGATCCTGGAAGGCGGCAACGCGTTGC
AGTTGGCCGAACAGGCAAAGCACGAAGGTGTCGCGGATCTGCGGCGCTCCGGTCTTTCGAAGGTGAAGGCCGGTATCACG
AGCCTTGAAGAGCTCAATCGGGTTACTAAGGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(194-461)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-151)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB L0GWW0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

53.31

100

0.536
  pilB Legionella pneumophila strain ERS1305867

53.054

100

0.532
  pilB Acinetobacter baylyi ADP1

52.715

100

0.527
  pilB Vibrio cholerae strain A1552

49.819

96.673

0.482
  pilF Neisseria gonorrhoeae MS11

47.818

100

0.48
  pilB Vibrio parahaemolyticus RIMD 2210633

51.562

89.667

0.462
  pilB Vibrio campbellii strain DS40M4

54.021

84.939

0.459
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.105

93.17

0.392
  pilF Thermus thermophilus HB27

42.045

92.469

0.389
  pilB/pilB1 Synechocystis sp. PCC 6803

40.494

92.119

0.373

Multiple sequence alignment