Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   J4N40_RS01840 Genome accession   NZ_CP071840
Coordinates   380910..382655 (+) Length   581 a.a.
NCBI ID   WP_033907306.1    Uniprot ID   -
Organism   Vibrio sp. SCSIO 43097     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 375910..387655
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J4N40_RS01820 (J4N40_01820) pilM 378218..379234 (+) 1017 WP_017821733.1 type IV pilus assembly protein PilM Machinery gene
  J4N40_RS01825 (J4N40_01825) pilN 379218..379793 (+) 576 WP_054577259.1 PilN domain-containing protein Machinery gene
  J4N40_RS01830 (J4N40_01830) pilO 379786..380379 (+) 594 WP_033907305.1 type 4a pilus biogenesis protein PilO Machinery gene
  J4N40_RS01835 (J4N40_01835) pilP 380369..380884 (+) 516 WP_005382766.1 pilus assembly protein PilP Machinery gene
  J4N40_RS01840 (J4N40_01840) pilQ 380910..382655 (+) 1746 WP_033907306.1 type IV pilus secretin PilQ family protein Machinery gene
  J4N40_RS01845 (J4N40_01845) aroK 382843..383361 (+) 519 WP_005381319.1 shikimate kinase AroK -
  J4N40_RS01850 (J4N40_01850) aroB 383394..384494 (+) 1101 WP_005381318.1 3-dehydroquinate synthase -
  J4N40_RS01855 (J4N40_01855) - 384547..386058 (+) 1512 WP_033907307.1 AAA family ATPase -
  J4N40_RS01860 (J4N40_01860) - 386128..386967 (+) 840 WP_005381301.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -

Sequence

Protein


Download         Length: 581 a.a.        Molecular weight: 63755.81 Da        Isoelectric Point: 4.4809

>NTDB_id=548148 J4N40_RS01840 WP_033907306.1 380910..382655(+) (pilQ) [Vibrio sp. SCSIO 43097]
MKMRQGLTRTPRILTGLFSLWLFVFLAPLAAAEEVSSNALKSIDFRTNKDKDAVIVVELASPAAIVDIKRVQEGLSIDLL
NTSVKDEQLYLLDVKDFSTVVESVEVFRYTSTTRLVAHINEEYMHDYRLTGRFLEIKVAKIKPNEKAPDKSILEREGKLI
SINFQDIPVRNVLQLIADYNQFNLVVSDSVEGNLTLRLDGVPWQQVLDIILQVKGLDKRVDGNVILVAPTDELDLREKQQ
LEKQKLTEEMGELSSEIIKVNFAKASDIAEMINGEGNISMLSERGSMTIDERTNSLLIRELPENIEVIREIIESLDIPVK
QVQIEARIVTINEGNMDELGIRWGFTSINGSNTVGGSIENNLATIGLYNGEGGDDGGDGDGVGIDDFLNVNLAATSPNAT
SLAFQVAKLGSDTLLDLELSALQQESKAEIISSPRLITTNKKPAYIEQGTEIPYLESSSSGATTVTFKKAVLSLKVTPQI
TPDNRLVLDLSVTQDRPGQVVKTGTGEAVAIDTQRIGTQVLVNNGETVVLGGIFQHSITNSVDKVPLLGDLPLLGALFRR
SYENVGKSELLIFVTPKVVIQ

Nucleotide


Download         Length: 1746 bp        

>NTDB_id=548148 J4N40_RS01840 WP_033907306.1 380910..382655(+) (pilQ) [Vibrio sp. SCSIO 43097]
ATGAAAATGAGACAAGGATTAACACGTACACCAAGGATACTTACTGGGCTCTTCTCCTTATGGTTGTTTGTATTTTTAGC
CCCTTTAGCGGCAGCTGAAGAAGTTTCGTCCAATGCCCTAAAAAGTATCGACTTTCGAACTAATAAAGATAAAGACGCCG
TGATTGTCGTCGAATTGGCGTCACCGGCCGCGATTGTAGATATCAAGCGTGTACAGGAAGGGTTAAGTATCGATTTGCTT
AACACTTCAGTAAAAGACGAGCAATTGTATCTCCTAGACGTGAAAGACTTTTCGACTGTGGTTGAGAGCGTAGAAGTGTT
TCGCTATACCTCAACTACGCGCTTGGTTGCGCACATCAATGAAGAATACATGCATGATTACCGTTTAACGGGGCGTTTTT
TGGAAATCAAAGTAGCGAAGATCAAACCCAATGAGAAAGCCCCAGATAAAAGCATTTTAGAAAGAGAAGGTAAGCTTATT
TCGATTAACTTCCAAGATATTCCTGTGCGAAACGTCTTGCAGCTTATTGCTGATTACAACCAATTCAATTTGGTTGTCTC
CGACTCTGTAGAAGGCAACCTCACGCTTCGTCTTGATGGTGTCCCTTGGCAGCAAGTGCTGGATATTATTTTGCAAGTTA
AGGGGTTAGATAAGCGTGTTGATGGCAACGTGATTTTAGTTGCTCCGACTGATGAGTTGGACTTGCGTGAGAAGCAGCAG
TTAGAAAAACAAAAATTGACGGAAGAAATGGGAGAGCTTTCATCTGAAATCATCAAAGTTAATTTTGCTAAAGCATCAGA
TATTGCGGAGATGATCAATGGAGAAGGAAATATCAGCATGCTGTCAGAGCGCGGCAGTATGACCATTGATGAGCGAACTA
ACTCGTTATTGATTAGAGAGTTACCTGAAAACATCGAGGTGATTCGAGAAATTATTGAGTCTTTGGATATTCCAGTTAAA
CAGGTTCAAATTGAGGCGCGTATTGTGACCATCAATGAAGGTAACATGGACGAGTTAGGGATTCGTTGGGGTTTTACATC
TATTAATGGCAGTAATACAGTGGGCGGTTCTATTGAGAACAACCTCGCCACGATCGGTCTTTATAATGGAGAAGGGGGCG
ACGACGGTGGTGACGGTGATGGAGTGGGGATCGATGACTTCCTAAATGTAAACTTAGCTGCAACCAGCCCGAATGCGACC
AGTCTTGCTTTCCAAGTTGCGAAGTTAGGATCAGACACACTACTTGATTTGGAACTTTCTGCGCTGCAACAAGAGTCTAA
AGCTGAGATTATCTCGAGCCCACGTTTGATCACTACCAATAAGAAACCAGCCTATATCGAACAAGGTACAGAGATTCCTT
ACCTAGAGTCATCATCAAGTGGTGCAACGACGGTGACGTTCAAAAAGGCGGTATTAAGTTTGAAGGTGACCCCGCAAATC
ACACCGGATAACCGCTTAGTGTTGGATTTAAGTGTCACGCAAGATAGACCTGGACAAGTCGTGAAGACAGGCACTGGTGA
AGCTGTTGCGATTGATACTCAACGTATCGGGACGCAAGTGTTAGTGAATAATGGTGAAACGGTCGTTCTTGGTGGTATTT
TTCAGCACAGCATTACTAACTCGGTGGACAAAGTGCCTTTGTTGGGCGATCTGCCGCTATTAGGTGCGCTTTTTCGTCGA
AGCTATGAAAATGTTGGCAAAAGTGAGCTGTTAATTTTCGTCACGCCTAAGGTGGTGATTCAGTAG

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(256-321)

Bacterial type II and III secretion system protein (Secretin)

(421-579)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Vibrio campbellii strain DS40M4

92.599

100

0.926
  pilQ Vibrio cholerae strain A1552

74.57

100

0.747
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

74.57

100

0.747
  pilQ Pseudomonas aeruginosa PAK

39.896

99.656

0.398
  pilQ Legionella pneumophila strain Lp02

40.608

96.213

0.391
  pilQ Legionella pneumophila strain ERS1305867

40.608

96.213

0.391
  comQ Acinetobacter baylyi ADP1

37.069

99.828

0.37
  pilQ Acinetobacter baumannii D1279779

35.906

100

0.368
  pilQ Acinetobacter baumannii strain A118

35.906

100

0.368