Detailed information NTG0054470

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: UC509_RS08625
• Genome accession: NC_019435
• Coordinates: 1721994..1722575 (-)
• Length: 193 a.a.
• NCBI ID: WP_259293135.1
• Uniprot ID: -
• Organism: Lactococcus cremoris subsp. cremoris UC509.9
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1716994..1727575

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
UC509_RS08600 (uc509_1720)	-	1717339..1717554 (-)	216	WP_014572212.1	F0F1 ATP synthase subunit C	-
UC509_RS08605 (uc509_1721)	-	1717737..1718513 (-)	777	WP_014572211.1	alpha/beta hydrolase family protein	-
UC509_RS08610 (uc509_1722)	-	1718796..1719100 (-)	305	Protein_1673	ComEC/Rec2 family competence protein	-
UC509_RS12660	-	1719121..1720012 (-)	892	Protein_1674	IS982-like element IS982B family transposase	-
UC509_RS13615	comEC	1720089..1720598 (-)	510	WP_228764033.1	MBL fold metallo-hydrolase	Machinery gene
UC509_RS08620 (uc509_1725)	comEC	1720595..1721866 (-)	1272	WP_324187032.1	ComEC/Rec2 family competence protein	Machinery gene
UC509_RS08625 (uc509_1727)	comEA	1721994..1722575 (-)	582	WP_259293135.1	helix-hairpin-helix domain-containing protein	Machinery gene
UC509_RS08630 (uc509_1728)	-	1722706..1724067 (-)	1362	WP_014572203.1	ABC transporter permease	-
UC509_RS08635 (uc509_1729)	-	1724064..1724996 (-)	933	WP_014572202.1	ABC transporter ATP-binding protein	-
UC509_RS08640 (uc509_1730)	-	1725102..1725500 (-)	399	WP_011676775.1	hypothetical protein	-
UC509_RS08645 (uc509_1731)	-	1725617..1726176 (-)	560	Protein_1681	GNAT family N-acetyltransferase	-
UC509_RS08650 (uc509_1732)	-	1726349..1727527 (-)	1179	WP_015082749.1	SLC13 family permease	-

Sequence

Protein

• Length: 193 a.a.
• Molecular weight: 21084.56 Da
• Isoelectric Point: 4.8079

>NTDB_id=54470 UC509_RS08625 WP_259293135.1 1721994..1722575(-) (comEA) [Lactococcus cremoris subsp. cremoris UC509.9]
MEFLYIFTNSSKQAENLSVENLSSSSTKSSVSKFNSSSEKNKNEIMVDLKGAVAKPNVYQISSDERLVDLIRQAGGFTDQ
ADQKSINLSSKLKDEEVIYVPKLGESLSSESTDSPISSSVSNQVSTTSGPKININKADLTELQKLTGIGQKKAQDIIDFR
MKNGDFKSIEDLGKVSGFGDKTLEKLKDEISID

Nucleotide

• Length: 582 bp

>NTDB_id=54470 UC509_RS08625 WP_259293135.1 1721994..1722575(-) (comEA) [Lactococcus cremoris subsp. cremoris UC509.9]
GTGGAATTTTTATATATTTTCACCAATTCATCAAAGCAAGCTGAAAATCTGTCAGTAGAAAATTTGAGCTCTAGTTCTAC
CAAAAGCTCTGTCAGTAAATTTAATAGCAGCAGTGAAAAAAATAAAAACGAAATTATGGTTGATTTGAAAGGAGCGGTTG
CAAAACCTAATGTATACCAAATTTCGTCAGATGAGCGCCTTGTTGATTTAATTAGGCAAGCGGGAGGATTTACTGACCAA
GCAGACCAAAAATCAATCAATCTGTCATCGAAACTTAAAGATGAAGAAGTGATTTATGTACCAAAACTTGGGGAAAGTTT
AAGTTCAGAAAGTACTGACAGTCCTATTAGCAGCTCTGTCAGTAATCAAGTTTCAACGACAAGTGGTCCAAAAATAAATA
TCAATAAAGCAGACCTGACAGAATTACAAAAACTGACTGGAATTGGTCAAAAAAAAGCGCAAGATATTATTGATTTTCGC
ATGAAAAATGGTGACTTTAAATCAATAGAGGATTTGGGTAAAGTATCTGGCTTTGGGGATAAAACATTAGAAAAATTGAA
AGATGAGATTTCTATTGATTAA

Domains

Predicted by InterproScan.

• SLBB domain (SLBB) (46-101)

• Helix-hairpin-helix motif (HHH_3) (129-190)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Lactococcus lactis subsp. cremoris KW2	93.229	99.482	0.927
comEA	Latilactobacillus sakei subsp. sakei 23K	40.385	100	0.435
comEA/celA/cilE	Streptococcus mitis SK321	45.856	93.782	0.43
comEA	Staphylococcus aureus N315	38.421	98.446	0.378
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	48.993	77.202	0.378
comEA/celA/cilE	Streptococcus pneumoniae R6	48.322	77.202	0.373
comEA/celA/cilE	Streptococcus pneumoniae Rx1	48.322	77.202	0.373
comEA/celA/cilE	Streptococcus pneumoniae D39	48.322	77.202	0.373
comEA	Staphylococcus aureus MW2	40.341	91.192	0.368
comEA/celA/cilE	Streptococcus mitis NCTC 12261	47.651	77.202	0.368