Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   J0J34_RS00720 Genome accession   NZ_CP071290
Coordinates   146109..147053 (+) Length   314 a.a.
NCBI ID   WP_206886610.1    Uniprot ID   -
Organism   Lactococcus garvieae strain LG791     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 141109..152053
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J0J34_RS00705 (J0J34_00705) - 142519..143973 (-) 1455 WP_206886357.1 GH25 family lysozyme -
  J0J34_RS00710 (J0J34_00710) guaB 144142..145623 (+) 1482 WP_003133714.1 IMP dehydrogenase -
  J0J34_RS00715 (J0J34_00715) rpsU 145746..145922 (+) 177 WP_003134539.1 30S ribosomal protein S21 -
  J0J34_RS00720 (J0J34_00720) comGA 146109..147053 (+) 945 WP_206886610.1 competence type IV pilus ATPase ComGA Machinery gene
  J0J34_RS00725 (J0J34_00725) comGB 146992..148011 (+) 1020 WP_276512710.1 competence type IV pilus assembly protein ComGB Machinery gene
  J0J34_RS00730 (J0J34_00730) comYC 148030..148335 (+) 306 WP_016171026.1 competence type IV pilus major pilin ComGC Machinery gene
  J0J34_RS10415 comGD 148310..148726 (+) 417 WP_014024177.1 competence type IV pilus minor pilin ComGD Machinery gene
  J0J34_RS10420 (J0J34_00735) comGE 148758..148991 (+) 234 WP_276512708.1 competence type IV pilus minor pilin ComGE -
  J0J34_RS00740 (J0J34_00740) comYF 148972..149412 (+) 441 WP_014024179.1 competence type IV pilus minor pilin ComGF Machinery gene
  J0J34_RS00745 (J0J34_00745) - 149645..150490 (+) 846 WP_014024181.1 metal ABC transporter solute-binding protein, Zn/Mn family -
  J0J34_RS00750 (J0J34_00750) - 150576..151445 (-) 870 WP_014570921.1 RluA family pseudouridine synthase -

Sequence

Protein


Download         Length: 314 a.a.        Molecular weight: 35897.19 Da        Isoelectric Point: 6.7735

>NTDB_id=544605 J0J34_RS00720 WP_206886610.1 146109..147053(+) (comGA) [Lactococcus garvieae strain LG791]
MIQVLAKTLLQKAVDQMVHDLYFVALEGKYSLYFRTATERRFERELDLEQGQALIAHMKFLSGMNLGESRRVQLGACTYV
LDKGEQRLRLSTVGDFHGQESLVIRLLHHQQNQLHFWNSEIFKSFIGGRGLYLFSGPVGSGKTSLMYKFAREHFKNQQVI
CIEDPVELVETEFLQLQVNKVIGNDYDALIKLSLRHRPDLLIVGEIRDSQTAKAVLRASLTGYTVFSTVHARSISGVILR
LKELGLTDWELQSSLQRVIYQRLIAGKGLLVYEKEKFEEWQPDEWNGQIDQLVADGFISAVTAAREKIEFSEAD

Nucleotide


Download         Length: 945 bp        

>NTDB_id=544605 J0J34_RS00720 WP_206886610.1 146109..147053(+) (comGA) [Lactococcus garvieae strain LG791]
ATGATACAGGTTTTGGCAAAAACCCTTTTACAAAAAGCAGTAGATCAGATGGTGCATGATCTTTATTTCGTAGCATTAGA
AGGAAAGTATTCTCTCTATTTTCGTACAGCAACAGAAAGGAGGTTTGAAAGAGAACTCGATCTTGAACAAGGGCAAGCAC
TGATTGCTCATATGAAATTTTTATCTGGTATGAATCTAGGAGAAAGTAGGCGGGTACAGTTGGGGGCTTGCACTTATGTT
TTAGATAAAGGTGAGCAGCGTTTACGTTTATCTACAGTCGGTGATTTTCATGGTCAAGAAAGCTTGGTTATCCGACTTCT
GCATCATCAACAAAACCAACTTCATTTTTGGAATTCAGAGATTTTTAAATCTTTTATTGGTGGGAGAGGGCTTTACCTGT
TTTCCGGTCCCGTAGGCTCAGGTAAGACCAGCCTTATGTACAAGTTTGCAAGGGAGCATTTTAAAAATCAACAAGTTATT
TGTATAGAAGATCCTGTGGAGTTAGTGGAAACTGAGTTTTTACAACTTCAAGTCAATAAAGTAATAGGGAATGATTACGA
TGCCTTGATTAAGCTCTCTCTTCGCCACAGGCCTGATTTACTCATTGTTGGTGAAATTAGGGACAGTCAAACGGCAAAAG
CTGTTCTTCGCGCAAGTTTGACGGGATATACAGTGTTTTCTACTGTTCATGCGCGCTCAATATCGGGTGTAATTTTACGG
CTAAAGGAACTTGGATTAACTGATTGGGAGCTACAATCCAGCCTTCAGCGGGTGATTTATCAGCGCTTAATTGCAGGAAA
GGGGTTGCTTGTTTATGAAAAAGAAAAGTTTGAAGAATGGCAACCAGATGAATGGAACGGCCAGATTGATCAGTTGGTTG
CAGATGGATTTATCTCAGCTGTTACAGCTGCGCGCGAAAAAATTGAGTTTAGCGAAGCAGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-264)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Lactococcus lactis subsp. cremoris KW2

59.873

100

0.599
  comYA Streptococcus gordonii str. Challis substr. CH1

53.651

100

0.538
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

53.355

99.682

0.532
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

53.035

99.682

0.529
  comGA/cglA/cilD Streptococcus pneumoniae D39

53.035

99.682

0.529
  comGA/cglA/cilD Streptococcus pneumoniae R6

53.035

99.682

0.529
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

53.035

99.682

0.529
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

52.077

99.682

0.519
  comYA Streptococcus mutans UA159

51.282

99.363

0.51
  comYA Streptococcus mutans UA140

51.282

99.363

0.51