Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB   Type   Machinery gene
Locus tag   J0J34_RS00725 Genome accession   NZ_CP071290
Coordinates   146992..148011 (+) Length   339 a.a.
NCBI ID   WP_276512710.1    Uniprot ID   -
Organism   Lactococcus garvieae strain LG791     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 141992..153011
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J0J34_RS00705 (J0J34_00705) - 142519..143973 (-) 1455 WP_206886357.1 GH25 family lysozyme -
  J0J34_RS00710 (J0J34_00710) guaB 144142..145623 (+) 1482 WP_003133714.1 IMP dehydrogenase -
  J0J34_RS00715 (J0J34_00715) rpsU 145746..145922 (+) 177 WP_003134539.1 30S ribosomal protein S21 -
  J0J34_RS00720 (J0J34_00720) comGA 146109..147053 (+) 945 WP_206886610.1 competence type IV pilus ATPase ComGA Machinery gene
  J0J34_RS00725 (J0J34_00725) comGB 146992..148011 (+) 1020 WP_276512710.1 competence type IV pilus assembly protein ComGB Machinery gene
  J0J34_RS00730 (J0J34_00730) comYC 148030..148335 (+) 306 WP_016171026.1 competence type IV pilus major pilin ComGC Machinery gene
  J0J34_RS10415 comGD 148310..148726 (+) 417 WP_014024177.1 competence type IV pilus minor pilin ComGD Machinery gene
  J0J34_RS10420 (J0J34_00735) comGE 148758..148991 (+) 234 WP_276512708.1 competence type IV pilus minor pilin ComGE -
  J0J34_RS00740 (J0J34_00740) comYF 148972..149412 (+) 441 WP_014024179.1 competence type IV pilus minor pilin ComGF Machinery gene
  J0J34_RS00745 (J0J34_00745) - 149645..150490 (+) 846 WP_014024181.1 metal ABC transporter solute-binding protein, Zn/Mn family -
  J0J34_RS00750 (J0J34_00750) - 150576..151445 (-) 870 WP_014570921.1 RluA family pseudouridine synthase -

Sequence

Protein


Download         Length: 339 a.a.        Molecular weight: 39235.92 Da        Isoelectric Point: 9.8252

>NTDB_id=544606 J0J34_RS00725 WP_276512710.1 146992..148011(+) (comGB) [Lactococcus garvieae strain LG791]
MDLSQLLQLRAKKLSLAKQIKLVQLMNNLFKSGFHLSEIIDFLERSGLTEAYFISKMRAGLLNGQSFSGILAKLKFNEDI
VTQLSLAESHGNVEYTLGLIEEKLRRALNIRKKLIQVATYPLVLLAFLIFIMLGLKNYLLPQLDENRGLATYVIQNLPSL
FLGCLFSLLVVFYLGRYYWKKKTALEAMRLMVKIPFVGRFVQLYLTAYFSREWGNLIAQAVDLRQICFIMQEQKSRIFKE
FGFELLHILDRGQKFEDALRLYPIFTKELALIVEYGELKSKLGKELMVFSEESWALFFERVERAMQLIQPIVFLLVALLI
ILIYAAMLLPIYSNMGNLI

Nucleotide


Download         Length: 1020 bp        

>NTDB_id=544606 J0J34_RS00725 WP_276512710.1 146992..148011(+) (comGB) [Lactococcus garvieae strain LG791]
ATGGATTTATCTCAGCTGTTACAGCTGCGCGCGAAAAAATTGAGTTTAGCGAAGCAGATTAAGCTTGTTCAACTTATGAA
TAATCTTTTCAAAAGTGGCTTTCACCTTTCTGAAATTATTGATTTTCTTGAACGGTCGGGTCTCACGGAAGCATACTTTA
TTTCCAAAATGCGTGCAGGTTTACTCAACGGGCAAAGTTTCTCTGGTATTTTAGCGAAGCTCAAATTTAATGAGGATATT
GTGACTCAGCTCTCTTTGGCCGAGTCTCATGGGAATGTTGAGTATACTTTAGGGTTGATTGAAGAAAAACTGAGACGCGC
TTTGAACATTCGCAAAAAGCTCATCCAAGTTGCCACTTACCCGCTCGTTCTTCTGGCATTTCTAATCTTTATCATGCTAG
GGTTGAAAAATTATTTACTGCCTCAACTGGACGAAAACAGAGGATTAGCAACTTATGTTATACAGAATCTTCCGTCCCTC
TTTTTAGGGTGCCTCTTCAGTTTGCTTGTTGTCTTTTATCTGGGCAGATACTATTGGAAGAAAAAAACTGCTTTAGAAGC
GATGAGGCTAATGGTCAAAATACCTTTTGTTGGCCGTTTTGTCCAGCTTTATTTGACAGCTTATTTTTCAAGAGAATGGG
GAAACTTAATTGCTCAAGCAGTCGATTTACGTCAGATTTGTTTTATTATGCAAGAACAAAAAAGTCGAATCTTCAAAGAA
TTTGGTTTTGAGCTCCTTCATATATTAGATAGGGGTCAGAAGTTTGAAGATGCCCTTCGACTTTACCCCATATTTACCAA
GGAGTTGGCATTAATTGTGGAATATGGTGAACTAAAGTCCAAGCTAGGGAAAGAGTTAATGGTTTTTTCTGAAGAAAGCT
GGGCGCTCTTTTTTGAAAGAGTTGAAAGAGCAATGCAACTTATCCAGCCTATCGTTTTCTTGCTCGTGGCGCTGTTGATT
ATTTTAATTTATGCTGCAATGTTACTTCCTATATATAGCAATATGGGAAACTTGATTTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(23-141)

Type II secretion system (T2SS), protein F (T2SSF)

(209-330)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB Lactococcus lactis subsp. cremoris KW2

53.846

99.705

0.537
  comGB/cglB Streptococcus mitis NCTC 12261

53.433

98.82

0.528
  comGB/cglB Streptococcus pneumoniae D39

52.239

98.82

0.516
  comGB/cglB Streptococcus pneumoniae R6

52.239

98.82

0.516
  comGB/cglB Streptococcus pneumoniae TIGR4

52.239

98.82

0.516
  comGB/cglB Streptococcus mitis SK321

52.239

98.82

0.516
  comGB/cglB Streptococcus pneumoniae Rx1

52.239

98.82

0.516
  comYB Streptococcus gordonii str. Challis substr. CH1

51.488

99.115

0.51
  comYB Streptococcus mutans UA140

51.429

92.92

0.478
  comYB Streptococcus mutans UA159

51.111

92.92

0.475