Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   J0J32_RS00660 Genome accession   NZ_CP071285
Coordinates   136424..137368 (+) Length   314 a.a.
NCBI ID   WP_003133715.1    Uniprot ID   -
Organism   Lactococcus garvieae strain LG728     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 131424..142368
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J0J32_RS00645 (J0J32_00645) - 132686..134287 (-) 1602 WP_017370492.1 GH25 family lysozyme -
  J0J32_RS00650 (J0J32_00650) guaB 134456..135937 (+) 1482 WP_003133714.1 IMP dehydrogenase -
  J0J32_RS00655 (J0J32_00655) rpsU 136060..136236 (+) 177 WP_003134539.1 30S ribosomal protein S21 -
  J0J32_RS00660 (J0J32_00660) comGA 136424..137368 (+) 945 WP_003133715.1 competence type IV pilus ATPase ComGA Machinery gene
  J0J32_RS00665 (J0J32_00665) comGB 137307..138326 (+) 1020 WP_263867708.1 competence type IV pilus assembly protein ComGB Machinery gene
  J0J32_RS00670 (J0J32_00670) comYC 138345..138650 (+) 306 WP_016171026.1 competence type IV pilus major pilin ComGC Machinery gene
  J0J32_RS00675 (J0J32_00675) comGD 138625..139041 (+) 417 WP_003133718.1 competence type IV pilus minor pilin ComGD Machinery gene
  J0J32_RS00680 (J0J32_00680) comGE 139073..139306 (+) 234 WP_229031266.1 competence type IV pilus minor pilin ComGE -
  J0J32_RS00685 (J0J32_00685) comGF 139287..139727 (+) 441 WP_003133720.1 competence type IV pilus minor pilin ComGF -
  J0J32_RS00690 (J0J32_00690) - 139960..140805 (+) 846 WP_003133722.1 zinc ABC transporter substrate-binding protein -
  J0J32_RS00695 (J0J32_00695) - 140891..141760 (-) 870 WP_003133725.1 RluA family pseudouridine synthase -

Sequence

Protein


Download         Length: 314 a.a.        Molecular weight: 35836.07 Da        Isoelectric Point: 6.6641

>NTDB_id=544561 J0J32_RS00660 WP_003133715.1 136424..137368(+) (comGA) [Lactococcus garvieae strain LG728]
MIQVLAKTLLQKAVDQMVHDLYFVALEGKYSLYFRTATERRFERELDLEQGQALIAHMKFLSGMNLGESRRVQLGACTYV
LDKGEQRLRLSTVGDFHGQESLVIRLLHHQQNQLHFWNSEIFKSFIGGRGLYLFSGPVGSGKTSLMYKFAREHFKNQQVI
CIEDPVELVETEFLQLQVNKVIGNDYDALIKLSLRHRPDLLIVGEIRDSQTAKAVLRASLTGYTVFSTVHARSISGVIAR
LKELGLTDWELQSSLQRVIYQRLIAGKGLLVYEKEKFEEWQPDEWNGQIDQLVADGFISAVTAAHEKIEFSEAD

Nucleotide


Download         Length: 945 bp        

>NTDB_id=544561 J0J32_RS00660 WP_003133715.1 136424..137368(+) (comGA) [Lactococcus garvieae strain LG728]
ATGATACAGGTTTTGGCAAAAACCCTTTTACAAAAAGCAGTAGATCAGATGGTGCATGATCTTTATTTCGTAGCATTAGA
AGGAAAGTATTCTCTCTATTTTCGTACAGCAACAGAAAGGAGGTTTGAAAGAGAACTCGATCTTGAACAAGGGCAAGCAC
TGATTGCTCATATGAAATTTTTATCTGGTATGAATCTAGGAGAAAGTAGGCGGGTACAGTTGGGGGCTTGCACTTATGTT
TTAGATAAAGGTGAGCAGCGTTTACGTTTATCTACAGTCGGTGATTTTCATGGTCAAGAAAGCTTGGTTATCCGACTTCT
GCATCATCAACAAAACCAACTTCATTTTTGGAATTCAGAGATTTTTAAATCTTTTATTGGGGGGAGAGGGCTTTACCTGT
TTTCCGGTCCCGTAGGCTCAGGTAAGACCAGCCTCATGTACAAGTTTGCAAGGGAGCATTTTAAAAATCAACAAGTTATT
TGTATAGAAGATCCCGTGGAGTTAGTGGAAACTGAGTTTTTACAACTTCAAGTCAATAAAGTAATAGGGAATGATTACGA
TGCCTTGATTAAGCTCTCTCTTCGCCACAGACCTGATTTACTCATTGTTGGTGAAATTAGGGACAGTCAGACGGCAAAAG
CTGTTCTCCGCGCAAGTTTGACGGGATATACAGTATTTTCTACTGTTCATGCGCGCTCAATATCGGGTGTAATTGCACGG
CTAAAGGAACTTGGGTTAACTGATTGGGAGCTACAATCCAGCCTTCAGCGGGTGATTTATCAGCGCTTAATTGCAGGAAA
GGGATTGCTTGTTTATGAAAAAGAAAAGTTTGAAGAATGGCAACCAGACGAATGGAATGGCCAGATTGATCAGTTGGTTG
CAGATGGATTTATCTCAGCTGTTACAGCTGCGCACGAAAAAATTGAGTTTAGCGAAGCAGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-264)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Lactococcus lactis subsp. cremoris KW2

59.554

100

0.596
  comYA Streptococcus gordonii str. Challis substr. CH1

53.651

100

0.538
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

53.355

99.682

0.532
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

53.035

99.682

0.529
  comGA/cglA/cilD Streptococcus pneumoniae D39

53.035

99.682

0.529
  comGA/cglA/cilD Streptococcus pneumoniae R6

53.035

99.682

0.529
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

53.035

99.682

0.529
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

52.396

99.682

0.522
  comYA Streptococcus mutans UA159

51.603

99.363

0.513
  comYA Streptococcus mutans UA140

51.603

99.363

0.513