Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB   Type   Machinery gene
Locus tag   J0J32_RS00665 Genome accession   NZ_CP071285
Coordinates   137307..138326 (+) Length   339 a.a.
NCBI ID   WP_263867708.1    Uniprot ID   -
Organism   Lactococcus garvieae strain LG728     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 132307..143326
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J0J32_RS00645 (J0J32_00645) - 132686..134287 (-) 1602 WP_017370492.1 GH25 family lysozyme -
  J0J32_RS00650 (J0J32_00650) guaB 134456..135937 (+) 1482 WP_003133714.1 IMP dehydrogenase -
  J0J32_RS00655 (J0J32_00655) rpsU 136060..136236 (+) 177 WP_003134539.1 30S ribosomal protein S21 -
  J0J32_RS00660 (J0J32_00660) comGA 136424..137368 (+) 945 WP_003133715.1 competence type IV pilus ATPase ComGA Machinery gene
  J0J32_RS00665 (J0J32_00665) comGB 137307..138326 (+) 1020 WP_263867708.1 competence type IV pilus assembly protein ComGB Machinery gene
  J0J32_RS00670 (J0J32_00670) comYC 138345..138650 (+) 306 WP_016171026.1 competence type IV pilus major pilin ComGC Machinery gene
  J0J32_RS00675 (J0J32_00675) comGD 138625..139041 (+) 417 WP_003133718.1 competence type IV pilus minor pilin ComGD Machinery gene
  J0J32_RS00680 (J0J32_00680) comGE 139073..139306 (+) 234 WP_229031266.1 competence type IV pilus minor pilin ComGE -
  J0J32_RS00685 (J0J32_00685) comGF 139287..139727 (+) 441 WP_003133720.1 competence type IV pilus minor pilin ComGF -
  J0J32_RS00690 (J0J32_00690) - 139960..140805 (+) 846 WP_003133722.1 zinc ABC transporter substrate-binding protein -
  J0J32_RS00695 (J0J32_00695) - 140891..141760 (-) 870 WP_003133725.1 RluA family pseudouridine synthase -

Sequence

Protein


Download         Length: 339 a.a.        Molecular weight: 39465.30 Da        Isoelectric Point: 9.6241

>NTDB_id=544562 J0J32_RS00665 WP_263867708.1 137307..138326(+) (comGB) [Lactococcus garvieae strain LG728]
MDLSQLLQLRTKKLSLAKQIKLIQLMNNLFKSGFHLSEIIDFLERSGLTEAYFISKMREGLLNGQSFSGILAKLKFYEDI
VTQLSLAESHGNVEYTLGLIEEKLRRVLNIRKKLILVATYPLVLLVFLIFIMLGLKNYLLPQLDENRGLATYVIQNLPSL
FLGCLFSLLVVFYLCRYYWKKKTALEAMRLMVKIPFVGRFVQLYLTAYFSREWGNLIAQAVDLRQICFIMQEQKSRIFKE
FGFELLQILDRGQKFEDALRLYPIFTKELALIVEYGELKSKLGKELMVFSEESWALFFERVERAMQLIQPIVFLLVALLI
ILIYAAMLLPIYSNMGNLI

Nucleotide


Download         Length: 1020 bp        

>NTDB_id=544562 J0J32_RS00665 WP_263867708.1 137307..138326(+) (comGB) [Lactococcus garvieae strain LG728]
ATGGATTTATCTCAGCTGTTACAGCTGCGCACGAAAAAATTGAGTTTAGCGAAGCAGATTAAGCTTATTCAACTTATGAA
TAATCTTTTCAAAAGTGGCTTTCACCTTTCTGAAATTATTGATTTTCTTGAACGGTCGGGTCTCACGGAAGCATACTTTA
TTTCCAAAATGCGTGAAGGTTTACTCAACGGGCAAAGTTTCTCCGGTATTTTAGCGAAGCTCAAATTTTATGAGGATATT
GTGACTCAGCTCTCTTTGGCCGAGTCTCATGGGAATGTTGAGTATACTTTAGGGTTGATTGAAGAAAAACTGAGACGCGT
TTTGAACATTCGCAAAAAGCTCATCCTAGTTGCCACTTACCCGCTCGTTCTTCTGGTATTTCTAATCTTTATCATGCTAG
GGTTGAAAAATTATTTACTACCTCAACTGGACGAAAACAGAGGATTAGCAACTTATGTTATACAGAATCTTCCGTCCCTC
TTTTTAGGGTGCCTCTTCAGTTTACTTGTTGTCTTTTATCTGTGCAGATACTATTGGAAGAAGAAAACTGCTTTAGAAGC
GATGAGGCTAATGGTCAAAATCCCATTTGTTGGCCGTTTTGTCCAGCTTTATTTGACGGCTTATTTTTCAAGAGAATGGG
GAAACTTAATTGCCCAAGCAGTTGATTTACGTCAGATTTGCTTTATTATGCAAGAACAAAAAAGTCGAATCTTCAAAGAA
TTTGGTTTTGAGCTCCTTCAAATATTAGATAGGGGTCAGAAGTTTGAAGACGCCCTTCGACTTTACCCCATATTTACCAA
GGAGTTGGCATTAATTGTAGAATATGGTGAACTAAAGTCTAAGCTAGGGAAAGAGTTAATGGTTTTTTCTGAAGAAAGCT
GGGCGCTCTTTTTTGAAAGAGTTGAAAGAGCAATGCAACTTATCCAGCCTATCGTTTTCTTGCTCGTGGCGCTGTTGATT
ATTTTAATTTATGCTGCAATGTTACTTCCTATATATAGCAATATGGGAAACTTGATTTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(209-330)

Type II secretion system (T2SS), protein F (T2SSF)

(23-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB Lactococcus lactis subsp. cremoris KW2

54.438

99.705

0.543
  comGB/cglB Streptococcus mitis NCTC 12261

53.134

98.82

0.525
  comGB/cglB Streptococcus pneumoniae D39

52.836

98.82

0.522
  comGB/cglB Streptococcus pneumoniae R6

52.836

98.82

0.522
  comGB/cglB Streptococcus pneumoniae TIGR4

52.836

98.82

0.522
  comGB/cglB Streptococcus mitis SK321

52.836

98.82

0.522
  comGB/cglB Streptococcus pneumoniae Rx1

52.836

98.82

0.522
  comYB Streptococcus gordonii str. Challis substr. CH1

52.096

98.525

0.513
  comYB Streptococcus mutans UA140

51.746

92.92

0.481
  comYB Streptococcus mutans UA159

51.429

92.92

0.478