Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGD   Type   Machinery gene
Locus tag   J0J32_RS00675 Genome accession   NZ_CP071285
Coordinates   138625..139041 (+) Length   138 a.a.
NCBI ID   WP_003133718.1    Uniprot ID   -
Organism   Lactococcus garvieae strain LG728     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 133625..144041
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J0J32_RS00650 (J0J32_00650) guaB 134456..135937 (+) 1482 WP_003133714.1 IMP dehydrogenase -
  J0J32_RS00655 (J0J32_00655) rpsU 136060..136236 (+) 177 WP_003134539.1 30S ribosomal protein S21 -
  J0J32_RS00660 (J0J32_00660) comGA 136424..137368 (+) 945 WP_003133715.1 competence type IV pilus ATPase ComGA Machinery gene
  J0J32_RS00665 (J0J32_00665) comGB 137307..138326 (+) 1020 WP_263867708.1 competence type IV pilus assembly protein ComGB Machinery gene
  J0J32_RS00670 (J0J32_00670) comYC 138345..138650 (+) 306 WP_016171026.1 competence type IV pilus major pilin ComGC Machinery gene
  J0J32_RS00675 (J0J32_00675) comGD 138625..139041 (+) 417 WP_003133718.1 competence type IV pilus minor pilin ComGD Machinery gene
  J0J32_RS00680 (J0J32_00680) comGE 139073..139306 (+) 234 WP_229031266.1 competence type IV pilus minor pilin ComGE -
  J0J32_RS00685 (J0J32_00685) comGF 139287..139727 (+) 441 WP_003133720.1 competence type IV pilus minor pilin ComGF -
  J0J32_RS00690 (J0J32_00690) - 139960..140805 (+) 846 WP_003133722.1 zinc ABC transporter substrate-binding protein -
  J0J32_RS00695 (J0J32_00695) - 140891..141760 (-) 870 WP_003133725.1 RluA family pseudouridine synthase -

Sequence

Protein


Download         Length: 138 a.a.        Molecular weight: 15806.49 Da        Isoelectric Point: 10.0791

>NTDB_id=544564 J0J32_RS00675 WP_003133718.1 138625..139041(+) (comGD) [Lactococcus garvieae strain LG728]
MKNVMYRIKALTLLESLLVLFLCSFVLFLFSGSVKQSVRIARAEIFILQFERLYKDTQRMAGLKGQQQTLSASNGALYSQ
EEMVKVPEEAEINDFSITFDQKSGNSSLQKITIFLPYQKKTISYQLEIGSGKYKKRIT

Nucleotide


Download         Length: 417 bp        

>NTDB_id=544564 J0J32_RS00675 WP_003133718.1 138625..139041(+) (comGD) [Lactococcus garvieae strain LG728]
ATGAAAAACGTAATGTACCGAATTAAAGCCTTGACTTTACTGGAGAGTTTACTCGTTCTTTTCCTATGCTCTTTTGTTCT
TTTTCTATTTTCAGGTTCGGTCAAACAAAGTGTGAGGATAGCACGCGCAGAGATTTTTATTTTACAATTTGAACGTCTTT
ATAAGGATACCCAACGCATGGCGGGGCTAAAGGGGCAGCAACAGACGTTGAGTGCGAGTAATGGTGCACTCTATAGCCAA
GAGGAAATGGTGAAAGTCCCAGAGGAGGCTGAAATAAATGATTTCTCTATTACTTTTGATCAAAAATCAGGCAATTCGAG
CCTACAAAAAATCACAATATTTCTTCCCTATCAGAAAAAAACAATCTCTTATCAATTGGAGATTGGAAGCGGAAAATATA
AGAAAAGAATCACTTAA

Domains


No domain identified.



Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGD Lactococcus lactis subsp. cremoris KW2

48.551

100

0.486
  comYD Streptococcus mutans UA140

40.909

95.652

0.391
  comYD Streptococcus mutans UA159

40.909

95.652

0.391
  comGD/cglD Streptococcus mitis SK321

39.535

93.478

0.37
  comGD/cglD Streptococcus pneumoniae TIGR4

38.76

93.478

0.362