Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   JWU58_RS04925 Genome accession   NZ_CP070503
Coordinates   1054583..1055800 (-) Length   405 a.a.
NCBI ID   WP_024014342.1    Uniprot ID   V8R166
Organism   Pseudomonas atacamensis strain SM1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1049583..1060800
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JWU58_RS04885 (JWU58_04885) - 1050421..1050594 (-) 174 WP_007909875.1 DUF3094 domain-containing protein -
  JWU58_RS04890 (JWU58_04890) - 1050646..1051173 (-) 528 WP_016773146.1 MOSC domain-containing protein -
  JWU58_RS04895 (JWU58_04895) - 1051170..1051796 (-) 627 WP_007909887.1 DUF1780 domain-containing protein -
  JWU58_RS04900 (JWU58_04900) - 1051914..1052603 (+) 690 WP_206421551.1 energy-coupling factor ABC transporter permease -
  JWU58_RS04905 (JWU58_04905) - 1052669..1052884 (+) 216 WP_016773148.1 hypothetical protein -
  JWU58_RS04910 (JWU58_04910) yacG 1052892..1053092 (-) 201 WP_016773149.1 DNA gyrase inhibitor YacG -
  JWU58_RS04915 (JWU58_04915) coaE 1053089..1053712 (-) 624 WP_130887103.1 dephospho-CoA kinase -
  JWU58_RS04920 (JWU58_04920) pilD 1053709..1054581 (-) 873 WP_206421552.1 prepilin peptidase Machinery gene
  JWU58_RS04925 (JWU58_04925) pilC 1054583..1055800 (-) 1218 WP_024014342.1 type II secretion system F family protein Machinery gene
  JWU58_RS04930 (JWU58_04930) pilB 1055803..1057500 (-) 1698 WP_206421553.1 type IV-A pilus assembly ATPase PilB Machinery gene
  JWU58_RS04935 (JWU58_04935) - 1057727..1058164 (+) 438 WP_122698547.1 pilin -
  JWU58_RS04940 (JWU58_04940) - 1058464..1058817 (+) 354 WP_016773155.1 BON domain-containing protein -
  JWU58_RS04945 (JWU58_04945) - 1059082..1059381 (-) 300 WP_071173628.1 DUF2845 domain-containing protein -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 43995.68 Da        Isoelectric Point: 10.1214

>NTDB_id=540297 JWU58_RS04925 WP_024014342.1 1054583..1055800(-) (pilC) [Pseudomonas atacamensis strain SM1]
MAVKAAKVSVYAWEGTDRKGSKVSGELSGQNPALIKAQLRKQGINPGKVRKKSASLLSLGKRIKPQDIALFTRQMATMMK
AGVPLLQSFDIIGEGFENPAMRKLVDEVKQEVAAGNSFATALRKKPQYFDELYCNLVDAGEQSGALDTLLERVATYKEKS
EALKAKIKKAMTYPTAVVLVAAVVTGILLVKVVPQFQSVFSGFGAELPGFTLMIISLSEFMQQWWWAILFALVAAFFGTR
HALKTSQALRDRRDAWLLKLPLVGTLMYKSAVARFARTLSTTFAAGVPLVEALDSVAGATGNVVFKRAVLRVRQDVSTGM
QLNFSMRSTGVFPNMAVQMTAIGEESGALDDMLDKVAGFYEAEVDNMVDNLTSLMEPFIMVVLGVIVGGLVVAMYLPIFQ
LGSAI

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=540297 JWU58_RS04925 WP_024014342.1 1054583..1055800(-) (pilC) [Pseudomonas atacamensis strain SM1]
ATGGCGGTCAAGGCAGCGAAAGTCAGCGTTTACGCCTGGGAAGGCACCGACCGCAAAGGCAGTAAAGTCAGCGGCGAGTT
GAGCGGGCAGAACCCCGCACTGATCAAGGCACAGCTGCGCAAACAAGGGATCAACCCGGGCAAGGTGCGCAAGAAATCCG
CTTCCCTGCTGAGCTTGGGCAAACGCATCAAACCGCAGGACATCGCCCTGTTCACCCGGCAGATGGCAACGATGATGAAG
GCCGGTGTACCGCTGTTGCAGTCCTTCGACATCATCGGCGAGGGCTTCGAAAACCCGGCCATGCGCAAGCTGGTCGACGA
GGTCAAACAGGAGGTCGCCGCCGGTAACAGCTTCGCCACGGCCCTGCGCAAGAAGCCGCAATACTTCGACGAGTTGTACT
GCAACCTGGTCGACGCCGGCGAACAGTCTGGTGCCCTCGACACTTTGCTCGAACGGGTCGCGACCTATAAGGAAAAAAGC
GAAGCGCTCAAGGCCAAGATCAAGAAGGCCATGACCTACCCCACCGCCGTGGTGCTGGTGGCGGCGGTGGTGACCGGCAT
TCTGCTGGTCAAGGTAGTGCCGCAGTTCCAGTCGGTATTCTCCGGGTTCGGCGCCGAACTGCCGGGCTTCACCCTGATGA
TCATCAGCCTGTCGGAATTCATGCAGCAGTGGTGGTGGGCGATTCTTTTTGCGCTGGTGGCAGCGTTTTTCGGCACTCGC
CACGCGTTGAAGACTTCGCAGGCCCTGCGCGACCGGCGCGATGCCTGGCTGCTGAAGCTGCCACTGGTGGGCACCTTGAT
GTACAAGTCCGCCGTTGCCCGCTTTGCCCGCACCCTGTCGACCACTTTCGCCGCCGGGGTGCCATTGGTGGAAGCGCTGG
ATTCGGTCGCCGGCGCCACCGGCAACGTGGTGTTCAAGCGCGCGGTGCTGCGCGTGCGGCAGGACGTCTCGACCGGCATG
CAGCTGAATTTTTCGATGCGCAGCACCGGTGTATTTCCGAACATGGCGGTGCAGATGACCGCCATCGGCGAGGAATCGGG
CGCACTGGATGACATGCTCGACAAGGTCGCCGGTTTTTATGAAGCGGAAGTCGATAACATGGTCGACAACCTCACCAGCC
TGATGGAGCCGTTCATCATGGTGGTGCTGGGGGTCATCGTCGGTGGACTGGTGGTGGCCATGTACCTGCCGATCTTCCAA
CTCGGCTCAGCGATCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(275-397)

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB V8R166

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

74.321

100

0.743
  pilC Acinetobacter baylyi ADP1

59.069

100

0.595
  pilC Acinetobacter baumannii D1279779

58.333

100

0.588
  pilC Legionella pneumophila strain ERS1305867

52.75

98.765

0.521
  pilC Vibrio cholerae strain A1552

44.949

97.778

0.44
  pilG Neisseria gonorrhoeae MS11

43.424

99.506

0.432
  pilG Neisseria meningitidis 44/76-A

42.68

99.506

0.425
  pilC Vibrio campbellii strain DS40M4

41.626

100

0.417
  pilC Thermus thermophilus HB27

39.85

98.519

0.393