Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   JWU58_RS04930 Genome accession   NZ_CP070503
Coordinates   1055803..1057500 (-) Length   565 a.a.
NCBI ID   WP_206421553.1    Uniprot ID   -
Organism   Pseudomonas atacamensis strain SM1     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1050803..1062500
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JWU58_RS04895 (JWU58_04895) - 1051170..1051796 (-) 627 WP_007909887.1 DUF1780 domain-containing protein -
  JWU58_RS04900 (JWU58_04900) - 1051914..1052603 (+) 690 WP_206421551.1 energy-coupling factor ABC transporter permease -
  JWU58_RS04905 (JWU58_04905) - 1052669..1052884 (+) 216 WP_016773148.1 hypothetical protein -
  JWU58_RS04910 (JWU58_04910) yacG 1052892..1053092 (-) 201 WP_016773149.1 DNA gyrase inhibitor YacG -
  JWU58_RS04915 (JWU58_04915) coaE 1053089..1053712 (-) 624 WP_130887103.1 dephospho-CoA kinase -
  JWU58_RS04920 (JWU58_04920) pilD 1053709..1054581 (-) 873 WP_206421552.1 prepilin peptidase Machinery gene
  JWU58_RS04925 (JWU58_04925) pilC 1054583..1055800 (-) 1218 WP_024014342.1 type II secretion system F family protein Machinery gene
  JWU58_RS04930 (JWU58_04930) pilB 1055803..1057500 (-) 1698 WP_206421553.1 type IV-A pilus assembly ATPase PilB Machinery gene
  JWU58_RS04935 (JWU58_04935) - 1057727..1058164 (+) 438 WP_122698547.1 pilin -
  JWU58_RS04940 (JWU58_04940) - 1058464..1058817 (+) 354 WP_016773155.1 BON domain-containing protein -
  JWU58_RS04945 (JWU58_04945) - 1059082..1059381 (-) 300 WP_071173628.1 DUF2845 domain-containing protein -
  JWU58_RS04950 (JWU58_04950) - 1059378..1062329 (-) 2952 WP_123443322.1 DUF748 domain-containing protein -

Sequence

Protein


Download         Length: 565 a.a.        Molecular weight: 62579.62 Da        Isoelectric Point: 6.1319

>NTDB_id=540298 JWU58_RS04930 WP_206421553.1 1055803..1057500(-) (pilB) [Pseudomonas atacamensis strain SM1]
MNDIALSGLAKQLVQAELLTETSARQSWAQAQRNRVSLVNYLVHNKLVNSRQIAEIASEHFGMALLDLHCLDKETQPKGL
VSEKLVRQHCALPLWRRGNKLFVGLSDPSNQQAISDIQFSTGLSTEAILVEDDKLSDAIDKFFDSHASGLEEMADVDLDG
LDVESIDDNKQDVIGLDADDAPVVRFVHKMLLDAIKSGSSDLHFEPYEKNYRVRMRTDGMLREVAKPPIQLSGRIAARLK
VMASLDISERRKPQDGRIKMRLSKSKSIDFRVNTLPTLWGEKVVIRILDPSSAQMGIDALGYEAEQKALYLAALKQPQGM
ILVTGPTGSGKTVSLYTGLNILNTVDINISTAEDPVEINMEGINQVNVNPRQGLDFAQALRSFLRQDPDVIMVGEIRDLE
TAEIAIKAAQTGHLVLSTLHTNSAAETLTRLHNMGIPGFNIATSVSLIIAQRLARKLCAHCKRPLEIPHETLLKEGFPEE
RIGHFTIYEPVGCDHCNGGYKGRVGIYEVVKNTPDLQRLIMAEGNSLEIDIQMRRDGFADLRTSGLHKAMQGITSLEEIN
RVTKD

Nucleotide


Download         Length: 1698 bp        

>NTDB_id=540298 JWU58_RS04930 WP_206421553.1 1055803..1057500(-) (pilB) [Pseudomonas atacamensis strain SM1]
ATGAATGACATCGCTCTGAGCGGTCTGGCCAAGCAATTGGTCCAGGCCGAACTGCTCACGGAAACAAGTGCCCGGCAATC
CTGGGCGCAGGCCCAGCGCAATCGCGTGTCGCTGGTCAATTACCTGGTGCACAACAAACTGGTCAACAGCCGGCAAATTG
CCGAGATCGCCTCGGAACATTTCGGCATGGCCCTGCTCGATCTGCATTGCCTCGACAAGGAGACTCAGCCCAAGGGGCTG
GTCAGTGAAAAACTGGTTCGTCAGCATTGCGCCCTGCCCTTGTGGCGGCGCGGCAACAAACTGTTCGTGGGCCTTTCCGA
CCCGAGCAATCAGCAGGCGATCAGCGACATCCAGTTCAGCACCGGCCTGAGCACCGAAGCGATTCTGGTCGAGGACGACA
AACTCAGCGACGCCATCGACAAGTTCTTCGACAGCCACGCCTCGGGCCTGGAGGAAATGGCCGATGTCGATCTCGACGGC
CTCGACGTCGAGTCCATTGATGACAACAAACAGGACGTGATCGGCCTCGATGCCGACGATGCCCCGGTCGTACGCTTCGT
GCACAAGATGCTGCTCGATGCGATCAAGAGTGGCTCCTCCGACCTGCATTTCGAGCCGTACGAAAAAAACTATCGGGTGC
GCATGCGCACCGACGGCATGCTGCGCGAGGTCGCCAAGCCGCCGATCCAGTTGTCCGGGCGTATTGCCGCACGTTTGAAG
GTCATGGCCAGTCTGGACATTTCCGAACGACGCAAACCCCAGGACGGGCGGATCAAGATGCGCCTGTCGAAAAGCAAATC
CATCGATTTTCGCGTCAACACCCTGCCGACCCTGTGGGGCGAAAAAGTGGTGATCCGGATTCTCGACCCGTCCAGTGCAC
AAATGGGCATCGATGCGCTGGGCTACGAAGCCGAACAAAAAGCCTTGTACCTGGCCGCGCTGAAACAGCCGCAAGGGATG
ATTCTGGTCACCGGCCCCACCGGTTCCGGTAAAACCGTGTCGCTGTACACCGGGCTGAACATTCTCAACACCGTCGACAT
CAACATTTCCACCGCCGAAGACCCGGTGGAAATCAACATGGAAGGCATCAACCAAGTCAACGTCAATCCCCGCCAAGGGC
TGGACTTCGCCCAGGCGCTGCGCTCATTTCTGCGTCAGGACCCGGACGTGATCATGGTCGGCGAGATCCGCGATCTGGAA
ACCGCCGAGATCGCGATCAAGGCCGCGCAGACCGGCCACCTGGTGCTGTCGACCCTGCACACCAACAGCGCAGCGGAAAC
CCTCACGCGCCTGCACAACATGGGCATTCCCGGTTTCAACATTGCCACCTCGGTCAGCCTGATCATCGCCCAGCGCCTGG
CGCGCAAGTTGTGCGCACACTGCAAGCGGCCGCTGGAAATCCCGCACGAAACCTTGCTCAAGGAAGGCTTCCCCGAGGAA
CGCATCGGCCATTTCACGATCTACGAGCCGGTCGGTTGCGATCACTGCAATGGCGGTTACAAAGGACGCGTGGGGATTTA
TGAAGTGGTAAAGAACACACCGGACCTGCAACGGCTGATCATGGCCGAGGGCAATTCGCTGGAAATCGACATTCAGATGC
GCCGCGACGGTTTCGCCGATCTGCGCACCTCCGGGCTGCACAAGGCGATGCAAGGCATCACCAGCCTTGAGGAAATCAAC
CGGGTCACCAAGGATTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(189-456)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

57.597

100

0.577
  pilB Acinetobacter baylyi ADP1

57.651

99.469

0.573
  pilB Legionella pneumophila strain ERS1305867

56.714

100

0.568
  pilB Vibrio cholerae strain A1552

51.237

100

0.513
  pilB Vibrio parahaemolyticus RIMD 2210633

50.714

99.115

0.503
  pilF Neisseria gonorrhoeae MS11

50.089

99.646

0.499
  pilB Vibrio campbellii strain DS40M4

49.822

99.469

0.496
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.649

94.336

0.365