Detailed information NTG0053363

Bioinformatically predicted

Overview

• Name: hexA
• Type: Machinery gene
• Locus tag: U722_RS08470
• Genome accession: NC_023073
• Coordinates: 1738424..1741009 (+)
• Length: 861 a.a.
• NCBI ID: WP_024085312.1
• Uniprot ID: -
• Organism: Bacillus amyloliquefaciens LFB112
• Function: DNA mismatch repair (predicted from homology) Homologous recombination

Genomic Context

Location: 1733424..1746009

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
U722_RS08450 (U722_08910)	-	1734222..1735400 (+)	1179	WP_088037143.1	glycine C-acetyltransferase	-
U722_RS08455 (U722_08915)	miaB	1735543..1737072 (+)	1530	WP_024085311.1	tRNA (N6-isopentenyl adenosine(37)-C2)-methylthiotransferase MiaB	-
U722_RS08460 (U722_08920)	ymcA	1737075..1737506 (+)	432	WP_003154113.1	RicAFT regulatory complex protein RicA family protein	Regulator
U722_RS08465 (U722_08925)	cotE	1737760..1738305 (+)	546	WP_003154111.1	outer spore coat protein CotE	-
U722_RS08470 (U722_08930)	hexA	1738424..1741009 (+)	2586	WP_024085312.1	DNA mismatch repair protein MutS	Machinery gene
U722_RS08475 (U722_08935)	hexB	1741025..1742902 (+)	1878	WP_003154106.1	DNA mismatch repair endonuclease MutL	Machinery gene
U722_RS08480 (U722_08940)	-	1743469..1744113 (+)	645	WP_024085313.1	hypothetical protein	-
U722_RS08485	-	1744305..1744727 (+)	423	WP_041482363.1	hypothetical protein	-
U722_RS20020	-	1744879..1745212 (+)	334	Protein_1650	HNH endonuclease	-
U722_RS20380	-	1745340..1745525 (+)	186	Protein_1651	hypothetical protein	-

Sequence

Protein

• Length: 861 a.a.
• Molecular weight: 97674.88 Da
• Isoelectric Point: 5.2532

>NTDB_id=53363 U722_RS08470 WP_024085312.1 1738424..1741009(+) (hexA) [Bacillus amyloliquefaciens LFB112]
MASYTPMIQQYLKIKAEHQDAFLFFRLGDFYEMFFEDAKKASQELEITLTSRDGGSADKIPMCGVPYHSAAAYIEQLIKK
GYKVAICEQTEDPKAAKGVVKREVVQLITPGTVMDGKGIHESENNFIASVSEFRNGYGLALSDLTTGENLAVFIERIEDV
MSEIYSVGAKEIVVSSKFNEHTAAQLKERCGATISIEDGEITERIEIAKHLPGEELTETFMRLYTYLQKTQKRSLDHLQP
VQVYELEEAMKIDLYSKRNLELTETIRSKSKKGSLLWLLDETKTAMGGRLLKQWIDRPLIRASQIEERQEMVETLINHLF
EREDLRERLKEVYDLERLAGRVAFGNVNARDLIQLKESLKQVPSIKELVGSLNHKKAKERAGLIDPCGDLLDLLEEALHE
SPPLSLKEGNLIKDGYHQKLDEYRDASKNGKDWIARLEQQERAYTGIRSLKVGFNKVFGYYIEVTKANLHLLEDGRYERK
QTLTNAERYITPELKEKEALILEAENNICELEYELFAGLREKVKQFIPRLQRLAKQMSELDALQCFATISENRHYTKPVF
SDNEVKVIEGRHPVVEKVMDSQEYVPNNCLMGDSREMLLITGPNMSGKSTYMRQIALLSIMAQIGCFVPAKEAVLPIFDQ
IFTRIGAADDLISGQSTFMVEMLEAKNAIVNATKDSLILFDEIGRGTSTYDGMALAQAIIEYVHDHIGAKTLFSTHYHEL
TVLEDKLPQLKNVHVRAEEYNGTVVFLHQIKEGAADKSYGIHVAQLAELPDDLISRAQEILKQLEQTGDKPELPAAPEKK
PAVKEEPAQLSFFADGEKEQKAPAVSNKEKQVLEAFKSINILDMTPLEAMNEMYELQKKLK

Nucleotide

• Length: 2586 bp

>NTDB_id=53363 U722_RS08470 WP_024085312.1 1738424..1741009(+) (hexA) [Bacillus amyloliquefaciens LFB112]
ATGGCTAGTTACACGCCTATGATACAGCAATACTTGAAGATAAAGGCAGAGCACCAAGATGCCTTTTTATTTTTTCGGCT
TGGTGACTTTTATGAAATGTTTTTTGAAGATGCAAAAAAAGCGTCGCAAGAGCTTGAAATTACACTGACAAGCAGAGACG
GAGGTTCGGCTGATAAAATTCCGATGTGCGGCGTGCCGTATCATTCCGCGGCGGCTTATATTGAACAGCTGATTAAAAAG
GGCTATAAAGTGGCGATCTGTGAGCAGACGGAAGATCCGAAGGCGGCTAAAGGCGTTGTAAAAAGAGAAGTCGTCCAGCT
GATCACCCCGGGAACCGTGATGGATGGAAAAGGCATCCATGAGTCGGAAAACAACTTTATCGCTTCCGTTTCCGAATTTC
GTAACGGATACGGTCTCGCTTTATCAGACTTAACGACCGGAGAAAACCTGGCCGTATTTATTGAGCGGATTGAAGATGTC
ATGTCAGAAATTTACTCAGTCGGCGCAAAGGAAATTGTCGTATCAAGCAAGTTTAACGAACATACGGCTGCTCAGCTGAA
AGAACGGTGCGGCGCAACGATTTCCATCGAAGACGGGGAAATAACAGAACGCATTGAAATCGCAAAGCACTTGCCGGGCG
AAGAGCTGACAGAGACGTTTATGCGTTTATATACGTATTTGCAGAAAACGCAAAAACGGAGCCTGGATCATCTGCAGCCG
GTTCAAGTATATGAATTGGAAGAAGCGATGAAAATTGACCTGTATTCAAAACGGAATCTTGAGCTGACGGAAACGATCCG
TTCGAAAAGCAAAAAAGGGTCATTGCTTTGGCTTTTAGACGAGACGAAAACAGCAATGGGCGGCCGGCTTCTCAAACAAT
GGATTGACCGCCCGCTGATCAGGGCATCCCAAATCGAAGAACGCCAGGAAATGGTCGAGACGCTGATCAATCATCTGTTT
GAGCGTGAAGATCTGCGCGAGCGCCTGAAGGAAGTATACGATTTAGAACGTCTGGCGGGCCGGGTCGCATTCGGAAACGT
CAACGCAAGAGATTTAATCCAGCTGAAAGAATCTTTAAAACAAGTGCCGAGCATTAAAGAGCTTGTAGGATCTCTTAACC
ATAAAAAAGCAAAAGAACGCGCCGGCTTGATTGATCCGTGCGGAGATTTGCTTGACCTGTTAGAAGAAGCCCTTCATGAA
AGCCCGCCGTTATCGTTAAAAGAAGGCAACTTAATTAAAGACGGCTATCATCAGAAGCTCGATGAGTACCGTGACGCCAG
CAAAAACGGTAAAGACTGGATTGCCAGACTGGAGCAGCAGGAACGCGCTTATACGGGCATCCGTTCATTAAAGGTCGGTT
TCAATAAAGTGTTCGGCTATTATATTGAAGTCACAAAAGCAAACCTTCATCTGCTTGAGGACGGGCGGTATGAGCGGAAG
CAGACCCTCACAAACGCCGAACGGTACATTACGCCGGAATTGAAGGAAAAAGAAGCGCTTATTTTAGAAGCGGAAAACAA
CATCTGCGAGCTGGAATATGAGCTTTTTGCCGGTCTGCGTGAAAAGGTCAAACAATTTATTCCGCGTCTGCAGCGTCTTG
CCAAACAAATGAGTGAGCTGGATGCGTTGCAATGCTTTGCTACGATCAGTGAAAACAGGCATTACACAAAGCCCGTCTTT
TCTGATAATGAAGTAAAAGTCATTGAAGGGAGACACCCCGTCGTTGAAAAGGTGATGGACAGTCAAGAATACGTGCCGAA
CAACTGCTTGATGGGCGATTCGCGGGAAATGCTCCTGATTACGGGTCCGAATATGTCAGGTAAAAGCACGTACATGAGAC
AGATCGCACTTCTATCGATCATGGCCCAGATCGGCTGTTTTGTGCCCGCAAAAGAAGCGGTTCTGCCGATTTTTGATCAA
ATCTTCACGAGAATCGGGGCAGCGGATGATTTGATTTCCGGACAAAGCACCTTTATGGTGGAAATGCTGGAAGCGAAAAA
CGCGATTGTGAACGCCACAAAAGACAGCCTGATCCTGTTTGATGAAATCGGCCGCGGGACATCAACGTACGACGGAATGG
CGCTGGCACAGGCGATTATTGAATATGTTCATGATCATATCGGCGCTAAAACACTGTTCAGCACGCATTATCATGAGCTG
ACGGTGCTTGAAGATAAGCTTCCTCAGCTGAAAAACGTTCATGTGCGTGCAGAGGAATATAACGGAACGGTCGTGTTTCT
CCATCAAATCAAAGAAGGAGCCGCAGATAAAAGTTACGGCATTCATGTCGCCCAGCTTGCGGAGCTCCCGGACGATCTGA
TCAGCCGCGCGCAGGAGATTTTGAAACAGCTTGAGCAGACGGGAGATAAACCGGAGCTTCCGGCGGCACCTGAGAAAAAG
CCCGCCGTCAAAGAAGAACCGGCACAGCTGTCCTTTTTTGCTGACGGTGAAAAAGAACAGAAGGCACCGGCGGTTTCTAA
TAAAGAAAAACAAGTGCTTGAAGCTTTTAAGTCAATCAATATTCTCGATATGACGCCGCTTGAGGCGATGAATGAAATGT
ACGAGCTGCAAAAAAAATTAAAATAG

Domains

Predicted by InterProScan.

• MutS domain I (MutS_I) (5-116)

• MutS domain III (MutS_III) (258-547)

• MutS family domain IV (MutS_IV) (415-506)

• MutS domain II (MutS_II) (125-239)

• MutS domain V (MutS_V) (598-785)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
hexA	Streptococcus pneumoniae R6	48.308	99.535	0.481
mutS	Pseudomonas stutzeri strain ATCC 17587	38.435	100	0.394