Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   JOS67_RS02125 Genome accession   NZ_CP069195
Coordinates   341795..343543 (+) Length   582 a.a.
NCBI ID   WP_006742777.1    Uniprot ID   A0AA92LSF7
Organism   Vibrio diabolicus strain SLV18     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 336795..348543
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JOS67_RS02105 (JOS67_02100) pilM 339103..340119 (+) 1017 WP_203346870.1 type IV pilus biogenesis protein PilM Machinery gene
  JOS67_RS02110 (JOS67_02105) pilN 340103..340678 (+) 576 WP_005395717.1 PilN domain-containing protein Machinery gene
  JOS67_RS02115 (JOS67_02110) pilO 340671..341264 (+) 594 WP_203346871.1 type 4a pilus biogenesis protein PilO Machinery gene
  JOS67_RS02120 (JOS67_02115) pilP 341254..341769 (+) 516 WP_203346872.1 pilus assembly protein PilP Machinery gene
  JOS67_RS02125 (JOS67_02120) pilQ 341795..343543 (+) 1749 WP_006742777.1 type IV pilus secretin PilQ Machinery gene
  JOS67_RS02130 (JOS67_02125) aroK 343731..344249 (+) 519 WP_005381319.1 shikimate kinase AroK -
  JOS67_RS02135 (JOS67_02130) aroB 344282..345382 (+) 1101 WP_046874636.1 3-dehydroquinate synthase -
  JOS67_RS02140 (JOS67_02135) - 345435..346949 (+) 1515 WP_203346873.1 SPOR domain-containing protein -
  JOS67_RS02145 (JOS67_02140) - 347019..347858 (+) 840 WP_203346874.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -

Sequence

Protein


Download         Length: 582 a.a.        Molecular weight: 63760.73 Da        Isoelectric Point: 4.4533

>NTDB_id=532811 JOS67_RS02125 WP_006742777.1 341795..343543(+) (pilQ) [Vibrio diabolicus strain SLV18]
MKMRQGLTRTPRILTGLFSLWLFVFLAPLVAAEEVSSNALKSIDFRTNKDKDAVIVVELASPAAIVDVKRVQEGLSIDLL
NTSVKDEQLYLLDVKDFSTVVESVEVFRDTSTTRLVAHINEEYTHDYRLTGRFLEIKVAKIKPNEKAPDKSILEKEGKLI
SINFQDIPVRNVLQLIADYNQFNLVVSDSVEGNLTLRLDGVPWQQVLDIILQVKGLDKRVDGNVILVAPTDELDLREKQQ
LEKQQLEQTMGELSSEIIKVNFAKASDIAEMINGEGNISMLSERGSMTIDERTNSLLIRELPENIEVIREIIESLDIPVK
QVQIEARIVTINEGNMDELGIRWGFTSINGNNTVGGSIENNLATIGLYNGGGEGEDGGDGDGVGIDDFLNVNLAATSPNA
TSIAFQVAKLGSDTLLDLELSALQQESKAEIISSPRLITTNKKPAYIEQGTEIPYLESSSSGATTVTFKKAVLSLKVTPQ
ITPDNRLVLDLSVTQDRPGQVVKTGTGEAVAIDTQRIGTQVLVNNGETVVLGGIFQHSITNSVDKVPLLGDLPLLGALFR
RSYENVGKSELLIFVTPKVVIQ

Nucleotide


Download         Length: 1749 bp        

>NTDB_id=532811 JOS67_RS02125 WP_006742777.1 341795..343543(+) (pilQ) [Vibrio diabolicus strain SLV18]
ATGAAAATGAGACAAGGATTAACACGTACACCAAGGATACTAACTGGGCTCTTCTCCTTATGGTTGTTTGTATTTTTAGC
CCCTTTAGTGGCAGCAGAAGAAGTATCGTCTAATGCCTTAAAGAGTATCGACTTTCGCACTAATAAAGATAAAGACGCCG
TTATTGTCGTTGAACTGGCGTCACCTGCCGCGATTGTCGATGTCAAACGCGTACAGGAAGGGTTAAGTATCGATTTGCTT
AATACTTCGGTAAAAGACGAGCAACTGTATCTCCTAGACGTAAAAGATTTCTCGACTGTGGTTGAAAGCGTAGAGGTGTT
TCGCGATACCTCAACTACGCGCTTGGTTGCGCACATTAATGAAGAATATACGCATGATTACCGTTTAACGGGGCGATTCT
TGGAAATCAAAGTGGCTAAGATCAAACCCAATGAGAAAGCCCCGGATAAAAGCATTCTAGAAAAAGAAGGCAAGCTCATT
TCAATTAACTTCCAAGATATTCCTGTACGTAACGTGTTACAGCTGATTGCTGACTACAACCAGTTCAACTTGGTTGTTTC
AGACTCCGTAGAAGGTAACCTCACACTTCGTCTTGATGGTGTTCCGTGGCAGCAAGTGCTGGATATTATTTTGCAAGTTA
AGGGGTTAGATAAGCGTGTTGATGGCAACGTGATTCTGGTTGCTCCAACAGATGAGCTTGATCTGCGCGAAAAACAGCAA
CTCGAAAAGCAGCAGCTAGAACAAACGATGGGAGAGCTTTCGTCTGAAATCATCAAGGTAAACTTCGCTAAAGCATCAGA
TATTGCAGAGATGATCAACGGAGAAGGGAACATCAGTATGTTGTCTGAGCGAGGCAGTATGACCATTGATGAGCGGACTA
ACTCGTTATTGATTCGTGAGCTACCTGAAAACATTGAAGTGATTCGAGAAATTATTGAATCTTTGGATATCCCGGTGAAG
CAGGTTCAAATTGAAGCGCGTATTGTGACCATCAATGAAGGCAACATGGACGAGTTGGGAATTCGCTGGGGCTTTACCTC
TATTAATGGCAATAACACGGTTGGTGGTTCTATCGAAAACAACCTTGCCACGATCGGTTTATATAACGGTGGTGGTGAAG
GAGAAGACGGTGGTGACGGTGATGGAGTAGGTATCGATGACTTCCTAAACGTAAACTTAGCTGCAACCAGTCCAAACGCG
ACCAGCATTGCTTTTCAGGTTGCAAAATTAGGCTCAGACACCTTGTTAGACCTCGAGCTTTCTGCGCTGCAACAAGAGTC
CAAAGCTGAGATTATCTCAAGCCCACGTTTGATCACTACTAATAAGAAGCCTGCCTATATTGAGCAAGGTACCGAGATAC
CTTATCTAGAGTCCTCATCAAGTGGTGCTACGACGGTAACGTTCAAAAAAGCAGTATTGAGTTTGAAGGTGACGCCACAA
ATTACACCGGATAACCGTCTTGTGTTGGACTTAAGCGTTACGCAAGATAGACCTGGACAAGTCGTGAAAACAGGTACTGG
TGAAGCCGTTGCGATTGATACTCAACGTATCGGGACGCAAGTGTTAGTAAATAACGGTGAAACCGTCGTTCTTGGTGGTA
TTTTCCAGCACAGCATTACTAACTCGGTGGACAAAGTGCCTTTGTTAGGCGATCTGCCGCTATTAGGTGCGCTTTTCCGT
CGAAGTTATGAAAATGTTGGCAAAAGTGAGCTGTTAATTTTCGTCACCCCTAAGGTGGTGATTCAGTAG

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(256-321)

Bacterial type II and III secretion system protein (Secretin)

(422-580)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Vibrio campbellii strain DS40M4

92.955

100

0.93
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

74.443

100

0.746
  pilQ Vibrio cholerae strain A1552

74.443

100

0.746
  pilQ Legionella pneumophila strain Lp02

40.787

96.048

0.392
  pilQ Legionella pneumophila strain ERS1305867

40.787

96.048

0.392
  pilQ Pseudomonas aeruginosa PAK

39.716

96.907

0.385
  comQ Acinetobacter baylyi ADP1

37.349

99.828

0.373
  pilQ Acinetobacter baumannii strain A118

36.409

100

0.373
  pilQ Acinetobacter baumannii D1279779

36.409

100

0.373