Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   JOS67_RS02105 Genome accession   NZ_CP069195
Coordinates   339103..340119 (+) Length   338 a.a.
NCBI ID   WP_203346870.1    Uniprot ID   -
Organism   Vibrio diabolicus strain SLV18     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 334103..345119
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JOS67_RS02090 (JOS67_02085) - 334536..335264 (-) 729 WP_005395721.1 redoxin family protein -
  JOS67_RS02095 (JOS67_02090) oxyR 335435..336346 (+) 912 WP_005395720.1 DNA-binding transcriptional regulator OxyR -
  JOS67_RS02100 (JOS67_02095) - 336398..338959 (-) 2562 WP_104974736.1 penicillin-binding protein 1A -
  JOS67_RS02105 (JOS67_02100) pilM 339103..340119 (+) 1017 WP_203346870.1 type IV pilus biogenesis protein PilM Machinery gene
  JOS67_RS02110 (JOS67_02105) pilN 340103..340678 (+) 576 WP_005395717.1 PilN domain-containing protein Machinery gene
  JOS67_RS02115 (JOS67_02110) pilO 340671..341264 (+) 594 WP_203346871.1 type 4a pilus biogenesis protein PilO Machinery gene
  JOS67_RS02120 (JOS67_02115) pilP 341254..341769 (+) 516 WP_203346872.1 pilus assembly protein PilP Machinery gene
  JOS67_RS02125 (JOS67_02120) pilQ 341795..343543 (+) 1749 WP_006742777.1 type IV pilus secretin PilQ Machinery gene
  JOS67_RS02130 (JOS67_02125) aroK 343731..344249 (+) 519 WP_005381319.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 37664.06 Da        Isoelectric Point: 6.9711

>NTDB_id=532807 JOS67_RS02105 WP_203346870.1 339103..340119(+) (pilM) [Vibrio diabolicus strain SLV18]
MDKLIVTGIDIGHNSLKAVVLKPTGNNYALLGYKEILLKEGIVAENNTINHQEIVKTLKLIKKELPFGAKKVAISVPDNS
VISKKLQIEQSLEDSEVEFAVIQAFSHQSPFPVEELSLDFVRLRAEDGLRGTDNYQVFATRKDVVDSRVDALQKSGLKPV
LVDVHSQSLGHIWKLTADRFPDKNKYCLLDIGTSSSSFTMFTEQGELFHKEFACGTRGANSPVQDDLMSDDAQEKTELFN
RQIVERVKRQMQLYTSINGSQNIKGIWLSGEGASTPMLAEELSQQLSLECELLNPLGLFEMKVSKRKRRAADWQHFSTAA
GLAIRGIHWLGGARALSH

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=532807 JOS67_RS02105 WP_203346870.1 339103..340119(+) (pilM) [Vibrio diabolicus strain SLV18]
ATGGATAAGCTAATCGTTACCGGCATCGATATAGGCCATAACAGCCTCAAAGCCGTAGTACTTAAACCCACAGGCAACAA
TTACGCTCTGTTGGGTTACAAAGAAATATTACTAAAGGAAGGTATTGTCGCTGAAAATAACACTATAAATCATCAAGAAA
TTGTAAAGACCCTAAAGCTGATTAAAAAAGAACTGCCTTTTGGTGCTAAAAAGGTCGCAATTTCAGTGCCTGATAATTCG
GTGATCAGTAAAAAACTGCAAATAGAACAAAGCCTTGAAGATAGCGAAGTTGAGTTTGCTGTGATTCAGGCATTTTCTCA
TCAATCGCCATTCCCTGTCGAAGAGCTGAGTTTAGACTTCGTCCGTCTTCGTGCCGAAGATGGGTTGCGTGGGACTGATA
ACTATCAAGTGTTTGCGACAAGAAAAGATGTGGTCGATAGTCGCGTGGATGCATTACAAAAATCCGGACTAAAACCCGTT
TTAGTGGACGTGCATTCACAAAGTCTTGGTCATATCTGGAAGCTCACCGCCGATCGCTTTCCTGACAAAAATAAGTACTG
CTTACTCGATATTGGCACCTCATCGAGCTCCTTTACCATGTTTACCGAACAAGGTGAGCTGTTTCACAAAGAGTTTGCTT
GCGGCACTCGAGGAGCAAACAGCCCTGTTCAAGATGATTTGATGTCCGATGATGCTCAGGAGAAAACAGAGCTGTTTAAT
CGCCAAATTGTGGAACGGGTGAAACGTCAGATGCAGCTCTACACTTCAATTAATGGCTCGCAAAATATCAAAGGTATCTG
GTTATCAGGGGAAGGAGCTTCGACACCAATGCTCGCGGAAGAGTTATCTCAGCAACTGTCACTAGAGTGTGAATTGCTAA
ACCCGCTAGGGCTATTTGAGATGAAGGTGTCCAAACGTAAACGTCGTGCCGCGGATTGGCAGCATTTCTCTACAGCCGCA
GGACTCGCGATTCGTGGCATTCATTGGCTAGGAGGTGCGCGTGCTTTATCGCATTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-326)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio campbellii strain DS40M4

82.249

100

0.822
  pilM Vibrio cholerae strain A1552

50.746

99.112

0.503