Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   JOS67_RS02115 Genome accession   NZ_CP069195
Coordinates   340671..341264 (+) Length   197 a.a.
NCBI ID   WP_203346871.1    Uniprot ID   -
Organism   Vibrio diabolicus strain SLV18     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 335671..346264
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JOS67_RS02100 (JOS67_02095) - 336398..338959 (-) 2562 WP_104974736.1 penicillin-binding protein 1A -
  JOS67_RS02105 (JOS67_02100) pilM 339103..340119 (+) 1017 WP_203346870.1 type IV pilus biogenesis protein PilM Machinery gene
  JOS67_RS02110 (JOS67_02105) pilN 340103..340678 (+) 576 WP_005395717.1 PilN domain-containing protein Machinery gene
  JOS67_RS02115 (JOS67_02110) pilO 340671..341264 (+) 594 WP_203346871.1 type 4a pilus biogenesis protein PilO Machinery gene
  JOS67_RS02120 (JOS67_02115) pilP 341254..341769 (+) 516 WP_203346872.1 pilus assembly protein PilP Machinery gene
  JOS67_RS02125 (JOS67_02120) pilQ 341795..343543 (+) 1749 WP_006742777.1 type IV pilus secretin PilQ Machinery gene
  JOS67_RS02130 (JOS67_02125) aroK 343731..344249 (+) 519 WP_005381319.1 shikimate kinase AroK -
  JOS67_RS02135 (JOS67_02130) aroB 344282..345382 (+) 1101 WP_046874636.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 197 a.a.        Molecular weight: 23070.51 Da        Isoelectric Point: 4.7906

>NTDB_id=532809 JOS67_RS02115 WP_203346871.1 340671..341264(+) (pilO) [Vibrio diabolicus strain SLV18]
MVDWQDLEIDEMAEWPLLPQLVVIALLIFLIQGFGYWFYLKPKEEQLNNLVQQEQTLKVALKIKANKVAALPQLQSQLDE
LTTRYELLLQQLPVQKELATMLASVNELGIDNKLTFTRIDWGEKQSEHFLYRLPLNIELTGDFHDIGHFSQAIATLPRII
TFKDVTWQRVSQESETLHFRVRANTYQFKPEETNNDK

Nucleotide


Download         Length: 594 bp        

>NTDB_id=532809 JOS67_RS02115 WP_203346871.1 340671..341264(+) (pilO) [Vibrio diabolicus strain SLV18]
ATGGTTGATTGGCAGGATCTCGAAATAGATGAAATGGCAGAGTGGCCATTGTTGCCTCAGCTTGTGGTTATCGCGCTGCT
GATTTTCCTCATTCAAGGTTTTGGCTATTGGTTTTACCTTAAGCCAAAAGAAGAGCAATTGAATAACTTGGTCCAGCAGG
AGCAGACGCTCAAGGTGGCACTGAAGATCAAAGCGAACAAAGTAGCTGCATTGCCTCAGCTTCAATCTCAATTGGATGAA
CTCACCACGCGTTACGAGTTATTGCTACAACAACTGCCCGTGCAAAAAGAGTTGGCTACGATGCTCGCTTCGGTGAATGA
ACTCGGTATCGACAATAAGTTGACCTTTACCCGTATCGACTGGGGTGAAAAGCAATCAGAGCATTTCCTCTATCGTCTTC
CTTTAAACATTGAGTTAACGGGTGATTTTCATGACATTGGACATTTTTCTCAGGCGATAGCAACGCTACCTCGAATCATC
ACGTTTAAAGATGTGACTTGGCAGCGTGTCAGCCAAGAAAGTGAAACTCTGCACTTTAGGGTACGAGCCAATACCTATCA
GTTTAAGCCAGAGGAGACGAACAATGACAAGTAA

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(47-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Vibrio campbellii strain DS40M4

88.421

96.447

0.853
  pilO Vibrio cholerae strain A1552

70.558

100

0.706