Detailed information    

insolico Bioinformatically predicted

Overview

Name   ssb   Type   Machinery gene
Locus tag   EFD32_RS10030 Genome accession   NC_018221
Coordinates   1992589..1993038 (-) Length   149 a.a.
NCBI ID   WP_014862323.1    Uniprot ID   -
Organism   Enterococcus faecalis D32     
Function   ssDNA binding (predicted from homology)   
DNA processing

Genomic Context

Location: 1987589..1998038
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EFD32_RS10005 - 1989174..1989434 (-) 261 WP_041161716.1 hypothetical protein -
  EFD32_RS15495 (EFD32_1926) - 1989609..1990151 (-) 543 WP_049798585.1 super-infection exclusion protein B -
  EFD32_RS15935 (EFD32_1927) - 1990488..1990586 (-) 99 Protein_1903 putative holin-like toxin -
  EFD32_RS10015 (EFD32_1928) - 1990952..1991206 (-) 255 WP_014862319.1 hypothetical protein -
  EFD32_RS10020 (EFD32_1929) - 1991304..1991567 (-) 264 WP_104837054.1 hypothetical protein -
  EFD32_RS15940 - 1991629..1991817 (-) 189 WP_080019841.1 hypothetical protein -
  EFD32_RS10025 (EFD32_1931) - 1991939..1992492 (-) 554 Protein_1907 thermonuclease family protein -
  EFD32_RS10030 (EFD32_1932) ssb 1992589..1993038 (-) 450 WP_014862323.1 single-stranded DNA-binding protein Machinery gene
  EFD32_RS10035 (EFD32_1933) - 1993163..1993360 (+) 198 WP_014862324.1 hypothetical protein -
  EFD32_RS10040 (EFD32_1934) - 1993431..1993694 (+) 264 WP_014862325.1 hypothetical protein -
  EFD32_RS16710 (EFD32_1935) - 1993712..1997122 (-) 3411 WP_014862326.1 SNF2-related protein -

Sequence

Protein


Download         Length: 149 a.a.        Molecular weight: 16839.68 Da        Isoelectric Point: 4.9116

>NTDB_id=52276 EFD32_RS10030 WP_014862323.1 1992589..1993038(-) (ssb) [Enterococcus faecalis D32]
MMNNVTLIGRATKDPDLRYTVSGTTVATFTLAVNRNYKNQNGDREADYIQCVIWRKPAETLANYVKKGTLFGISGRLQTR
NYENQQGQRVYVTEVIVESFYLLESRSQAESREQKTTTSQTPPMDNTMGNNDDPFGGNPINISDDDLPF

Nucleotide


Download         Length: 450 bp        

>NTDB_id=52276 EFD32_RS10030 WP_014862323.1 1992589..1993038(-) (ssb) [Enterococcus faecalis D32]
ATGATGAATAATGTGACATTAATTGGAAGAGCGACGAAAGACCCAGATTTGCGCTATACAGTTAGTGGAACAACTGTAGC
AACATTTACTCTTGCCGTAAATCGTAATTATAAAAACCAGAATGGTGATCGTGAAGCAGATTACATTCAGTGTGTGATTT
GGCGTAAGCCAGCAGAAACGTTAGCTAATTATGTAAAAAAGGGAACATTGTTTGGAATTTCAGGAAGACTTCAAACTCGA
AACTATGAAAATCAGCAAGGACAACGAGTTTATGTGACAGAAGTTATTGTAGAAAGTTTTTATTTGTTGGAATCACGTTC
GCAAGCAGAATCGAGAGAACAAAAAACGACAACGAGTCAGACTCCACCTATGGATAATACTATGGGAAATAATGATGATC
CATTTGGTGGAAACCCAATAAATATTTCAGATGATGACTTACCGTTCTGA

Domains

Predicted by InterproScan.

Single-strand binding protein family (SSB)

(2-102)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ssb Latilactobacillus sakei subsp. sakei 23K

53.757

100

0.624
  ssbA Bacillus subtilis subsp. subtilis str. 168

52.326

100

0.604
  ssbB/cilA Streptococcus pneumoniae TIGR4

53.097

75.839

0.403
  ssbB Streptococcus sobrinus strain NIDR 6715-7

43.704

90.604

0.396
  ssbB Bacillus subtilis subsp. subtilis str. 168

55.66

71.141

0.396
  ssbB/cilA Streptococcus pneumoniae D39

52.212

75.839

0.396
  ssbB/cilA Streptococcus pneumoniae Rx1

52.212

75.839

0.396
  ssbB/cilA Streptococcus pneumoniae R6

52.212

75.839

0.396
  ssbB/cilA Streptococcus mitis NCTC 12261

52.212

75.839

0.396
  ssbB/cilA Streptococcus mitis SK321

52.212

75.839

0.396
  ssbA Streptococcus mutans UA159

41.481

90.604

0.376

Multiple sequence alignment