Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   JG479_RS14370 Genome accession   NZ_CP066804
Coordinates   3231728..3232957 (+) Length   409 a.a.
NCBI ID   WP_024597567.1    Uniprot ID   -
Organism   Pseudoalteromonas sp. LC2018020214     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3226728..3237957
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JG479_RS14345 (JG479_14345) ampE 3226738..3227589 (-) 852 WP_200219548.1 beta-lactamase regulator AmpE -
  JG479_RS14350 (JG479_14350) ampD 3227603..3228157 (-) 555 WP_200219549.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  JG479_RS14355 (JG479_14355) nadC 3228484..3229329 (+) 846 WP_200219550.1 carboxylating nicotinate-nucleotide diphosphorylase -
  JG479_RS14360 (JG479_14360) - 3229819..3230232 (+) 414 WP_200219551.1 pilin -
  JG479_RS14365 (JG479_14365) - 3230296..3231639 (+) 1344 WP_200219552.1 O-antigen ligase family protein -
  JG479_RS14370 (JG479_14370) pilC 3231728..3232957 (+) 1230 WP_024597567.1 type II secretion system F family protein Machinery gene
  JG479_RS14375 (JG479_14375) pilD 3233121..3234014 (+) 894 WP_200219553.1 A24 family peptidase Machinery gene
  JG479_RS14380 (JG479_14380) coaE 3234016..3234657 (+) 642 WP_200219554.1 dephospho-CoA kinase -
  JG479_RS14385 (JG479_14385) pilB 3234784..3236460 (+) 1677 WP_149603263.1 type IV-A pilus assembly ATPase PilB Machinery gene
  JG479_RS14390 (JG479_14390) yacG 3236563..3236793 (+) 231 WP_002957774.1 DNA gyrase inhibitor YacG -
  JG479_RS14395 (JG479_14395) - 3236866..3237354 (-) 489 WP_039488264.1 Spy/CpxP family protein refolding chaperone -

Sequence

Protein


Download         Length: 409 a.a.        Molecular weight: 44306.36 Da        Isoelectric Point: 9.8363

>NTDB_id=522670 JG479_RS14370 WP_024597567.1 3231728..3232957(+) (pilC) [Pseudoalteromonas sp. LC2018020214]
MAKKQQDKGLDTFQWVGVSARGKKLEGELTGNSIALVKAQLRKQGITPSKVKRKAKPLFGIQSVQKVTPKDIALLTRQIA
TMLMAGVPLIQAIEMIGSGSTNKSVAKLMETIADEVKAGQPLSTALRKHPRYFDDLYCDLVASGEQSGALDKIFDRVALY
KEKSEALKSKIKKAMFYPISVLVIAGIVTSILLIFVVPQFQDIFAGFGAELPAFTLFVIGISDFMQAYWWMILIAIVAFG
YAFKEAKLRSLKLRDATDRAILKLPVIGMILNKAAVARYARTLSTTFAAGVPLVDALDSAAGASGNAVYRYAILDIKVEV
SSGNQMNWAMRNSKIFPDMVIQMVAIGEESGSLDGMLAKVATIYEQEVDDAVDGLSSLLEPLIMAVLGVLVGGLIVAMYL
PIFQLGSVI

Nucleotide


Download         Length: 1230 bp        

>NTDB_id=522670 JG479_RS14370 WP_024597567.1 3231728..3232957(+) (pilC) [Pseudoalteromonas sp. LC2018020214]
ATGGCAAAAAAACAACAAGATAAAGGTCTCGACACTTTTCAGTGGGTAGGCGTTAGTGCACGAGGGAAGAAACTTGAAGG
TGAGCTTACAGGTAATAGTATTGCACTAGTGAAAGCTCAGCTTCGTAAGCAAGGTATCACTCCTTCAAAAGTGAAGCGTA
AAGCAAAACCACTGTTTGGTATTCAAAGTGTTCAAAAGGTAACCCCTAAAGATATAGCACTACTTACTCGCCAAATTGCG
ACTATGTTGATGGCGGGCGTTCCTTTAATACAGGCCATTGAAATGATTGGCTCTGGCTCGACCAATAAAAGTGTCGCCAA
ATTAATGGAGACTATTGCTGATGAAGTAAAAGCGGGTCAACCGTTATCTACAGCACTTAGAAAACACCCACGTTATTTTG
ATGATTTATATTGCGACCTTGTAGCCTCTGGCGAGCAATCTGGTGCACTTGATAAAATATTTGACCGAGTTGCTTTATAT
AAAGAAAAGTCCGAAGCGCTAAAATCAAAAATTAAAAAAGCGATGTTCTATCCTATTTCAGTATTAGTAATTGCCGGCAT
TGTAACCTCAATACTATTAATATTTGTAGTGCCGCAGTTTCAAGATATATTTGCAGGTTTTGGGGCAGAGCTTCCTGCAT
TCACTTTGTTTGTTATTGGCATATCAGACTTTATGCAAGCATATTGGTGGATGATATTAATCGCTATTGTAGCTTTTGGA
TACGCTTTTAAAGAAGCCAAGCTTAGAAGTTTAAAACTCAGAGATGCAACCGACCGAGCCATACTAAAACTGCCTGTTAT
TGGCATGATTTTAAATAAAGCAGCTGTTGCTCGCTATGCACGAACTCTTTCGACTACGTTTGCTGCAGGTGTGCCGCTGG
TTGATGCGCTTGACTCTGCCGCAGGCGCTTCAGGTAACGCTGTTTATCGATATGCCATTTTAGATATTAAAGTTGAAGTG
AGCTCTGGTAATCAAATGAACTGGGCAATGCGTAACTCAAAAATATTCCCTGATATGGTAATACAAATGGTTGCGATTGG
TGAAGAATCTGGCTCACTAGACGGTATGCTTGCTAAAGTAGCTACTATTTACGAGCAAGAAGTTGATGATGCAGTAGACG
GCCTTTCAAGCCTACTTGAGCCACTTATTATGGCTGTACTAGGTGTACTTGTTGGTGGCTTGATTGTTGCGATGTACTTA
CCTATTTTCCAACTTGGCTCTGTTATTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(76-198)

Type II secretion system (T2SS), protein F (T2SSF)

(280-401)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

62.972

97.066

0.611
  pilC Acinetobacter baylyi ADP1

55.392

99.756

0.553
  pilC Acinetobacter baumannii D1279779

56.25

97.8

0.55
  pilC Legionella pneumophila strain ERS1305867

55.75

97.8

0.545
  pilC Vibrio cholerae strain A1552

45.209

99.511

0.45
  pilG Neisseria meningitidis 44/76-A

44.757

95.599

0.428
  pilG Neisseria gonorrhoeae MS11

44.501

95.599

0.425
  pilC Vibrio campbellii strain DS40M4

43.25

97.8

0.423
  pilC Thermus thermophilus HB27

36.881

98.778

0.364