Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   JG479_RS14385 Genome accession   NZ_CP066804
Coordinates   3234784..3236460 (+) Length   558 a.a.
NCBI ID   WP_149603263.1    Uniprot ID   -
Organism   Pseudoalteromonas sp. LC2018020214     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3229784..3241460
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JG479_RS14360 (JG479_14360) - 3229819..3230232 (+) 414 WP_200219551.1 pilin -
  JG479_RS14365 (JG479_14365) - 3230296..3231639 (+) 1344 WP_200219552.1 O-antigen ligase family protein -
  JG479_RS14370 (JG479_14370) pilC 3231728..3232957 (+) 1230 WP_024597567.1 type II secretion system F family protein Machinery gene
  JG479_RS14375 (JG479_14375) pilD 3233121..3234014 (+) 894 WP_200219553.1 A24 family peptidase Machinery gene
  JG479_RS14380 (JG479_14380) coaE 3234016..3234657 (+) 642 WP_200219554.1 dephospho-CoA kinase -
  JG479_RS14385 (JG479_14385) pilB 3234784..3236460 (+) 1677 WP_149603263.1 type IV-A pilus assembly ATPase PilB Machinery gene
  JG479_RS14390 (JG479_14390) yacG 3236563..3236793 (+) 231 WP_002957774.1 DNA gyrase inhibitor YacG -
  JG479_RS14395 (JG479_14395) - 3236866..3237354 (-) 489 WP_039488264.1 Spy/CpxP family protein refolding chaperone -
  JG479_RS14400 (JG479_14400) - 3237544..3238233 (+) 690 WP_002957776.1 response regulator -
  JG479_RS14405 (JG479_14405) - 3238211..3239626 (+) 1416 WP_002957777.1 ATP-binding protein -
  JG479_RS14410 (JG479_14410) - 3239630..3240577 (+) 948 WP_138489704.1 alpha/beta fold hydrolase -
  JG479_RS14415 (JG479_14415) - 3240540..3241454 (-) 915 WP_029753364.1 cation diffusion facilitator family transporter -

Sequence

Protein


Download         Length: 558 a.a.        Molecular weight: 62257.43 Da        Isoelectric Point: 5.1531

>NTDB_id=522672 JG479_RS14385 WP_149603263.1 3234784..3236460(+) (pilB) [Pseudoalteromonas sp. LC2018020214]
MNINSPLLRKFITLGRIDADTVKNKQNEFASTAELISKCGKIDSKDLAEQCIDLFRVPYFDLKDFDPTRIPADLVKEKLI
RKHHILPLVQKDRKVYIAASDPTDYGAFENFEFSTGLSCEIVVVDYIQLDNKIEQLLDATGSLNLTDDEFQEFADLDSES
QRDTAPQDDDKDDAPIIVYINKILMDAIKKGASDLHFEPYEHKYRIRFRIDGILHEVASPPNTLATKLSARIKVMSRLDI
AEKRKPQDGRIKLKITERKSIDFRVSTMPTLWGEKIVMRILDSSSAMLGIDVLGYEPEQKKLYMDALAQPQGMILVTGPT
GSGKTVSLYTGLNILNQPERNISTAEDPVEINLEGVNQVQINIKADMTFANALRAFLRQDPDVVMVGEIRDLETAEISIK
AAQTGHLVLSTLHTNSAPETITRLLNMGVPAYNVASSISLIIAQRLARRLCPKCKTPEQLPSEELARQGFSAEQIEEMTL
YMPKGCDSCTDGYKGRVGIYEVMQITPEIAQIIMRGGNSLEIADVSLKAGFNNLRLSGLRKAADGLTSLGEINRVTNF

Nucleotide


Download         Length: 1677 bp        

>NTDB_id=522672 JG479_RS14385 WP_149603263.1 3234784..3236460(+) (pilB) [Pseudoalteromonas sp. LC2018020214]
ATGAATATTAACTCACCGTTATTGAGAAAATTTATAACCCTTGGCCGCATTGATGCCGACACGGTTAAAAACAAACAAAA
TGAATTTGCCTCAACAGCTGAGCTTATTTCTAAATGTGGAAAAATCGATAGTAAAGATTTAGCTGAACAATGTATTGATT
TGTTTCGCGTACCTTATTTTGATTTAAAAGATTTTGATCCTACAAGAATCCCGGCCGATCTCGTAAAAGAAAAACTCATA
CGTAAACATCACATTTTACCGCTTGTTCAAAAAGATCGAAAAGTTTATATAGCAGCATCAGACCCAACAGATTACGGCGC
ATTCGAAAACTTTGAGTTTAGTACCGGTCTTTCATGCGAAATTGTTGTTGTTGATTATATTCAACTCGATAACAAAATTG
AGCAGTTGCTAGATGCCACTGGCAGCTTAAATCTAACTGATGACGAGTTTCAAGAATTTGCTGATTTAGACTCAGAAAGC
CAAAGAGATACAGCACCACAAGATGACGATAAAGATGATGCGCCTATCATCGTATATATCAACAAAATTTTAATGGATGC
CATTAAAAAAGGCGCCTCTGATTTACACTTTGAACCGTATGAGCACAAGTACCGTATTCGCTTTCGTATTGATGGTATTT
TGCATGAAGTTGCAAGCCCACCCAATACATTAGCCACCAAGCTTTCTGCGCGTATTAAGGTTATGTCGAGACTCGATATT
GCCGAAAAACGCAAACCACAAGATGGTCGTATTAAACTAAAAATTACTGAACGCAAAAGTATCGATTTTCGTGTAAGTAC
CATGCCTACCTTGTGGGGCGAAAAAATAGTAATGCGTATTCTTGATTCATCAAGCGCTATGTTAGGCATTGATGTATTAG
GCTATGAGCCTGAGCAGAAAAAGCTCTACATGGATGCGCTTGCCCAGCCTCAAGGGATGATACTAGTAACAGGGCCAACC
GGTTCTGGTAAAACAGTATCGCTATACACAGGTTTAAATATATTAAACCAACCTGAGCGTAATATTAGTACTGCGGAAGA
TCCGGTTGAGATTAACCTTGAAGGTGTAAACCAAGTACAAATAAATATTAAAGCCGACATGACTTTTGCCAACGCACTGC
GCGCATTTTTACGTCAAGATCCTGATGTAGTTATGGTAGGTGAAATACGTGACTTAGAGACCGCAGAAATATCAATAAAA
GCGGCGCAAACAGGTCACTTGGTTTTATCAACATTGCATACAAACTCAGCGCCGGAAACAATCACTCGTTTATTAAATAT
GGGTGTACCCGCTTATAACGTAGCAAGTTCGATTAGCTTAATAATAGCCCAGCGTTTAGCGCGTCGCTTGTGCCCTAAAT
GTAAAACGCCAGAACAACTTCCGAGTGAAGAACTTGCTCGCCAAGGATTTAGTGCCGAGCAAATTGAAGAAATGACGTTA
TATATGCCTAAAGGGTGTGATAGCTGTACTGATGGTTACAAAGGGCGTGTGGGTATTTACGAAGTAATGCAAATTACTCC
TGAAATTGCCCAAATTATTATGCGCGGTGGTAATTCGCTTGAAATAGCAGACGTATCACTTAAAGCCGGTTTTAATAATT
TACGTTTATCAGGTTTACGCAAAGCAGCCGATGGCCTTACATCCCTTGGTGAAATTAACCGCGTAACCAATTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(182-449)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(40-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

58.869

91.935

0.541
  pilB Acinetobacter baylyi ADP1

59.01

90.502

0.534
  pilB Legionella pneumophila strain ERS1305867

52.612

96.057

0.505
  pilB Vibrio cholerae strain A1552

52.336

95.878

0.502
  pilB Vibrio parahaemolyticus RIMD 2210633

51.311

95.699

0.491
  pilB Vibrio campbellii strain DS40M4

51.215

95.878

0.491
  pilF Neisseria gonorrhoeae MS11

47.67

100

0.477
  pilB/pilB1 Synechocystis sp. PCC 6803

37.299

100

0.416
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

37.455

98.566

0.369