Detailed information    

insolico Bioinformatically predicted

Overview

Name   comYA   Type   Machinery gene
Locus tag   JF649_RS00645 Genome accession   NZ_CP066541
Coordinates   102019..102957 (+) Length   312 a.a.
NCBI ID   WP_063812149.1    Uniprot ID   -
Organism   Streptococcus pyogenes strain MGAS270     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 97019..107957
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JF649_RS00635 (JF649_00640) rpoC 97768..101409 (+) 3642 WP_032460334.1 DNA-directed RNA polymerase subunit beta' -
  JF649_RS00640 (JF649_00645) - 101561..101926 (+) 366 WP_002986560.1 DUF1033 family protein -
  JF649_RS00645 (JF649_00650) comYA 102019..102957 (+) 939 WP_063812149.1 competence type IV pilus ATPase ComGA Machinery gene
  JF649_RS00650 (JF649_00655) comYB 102893..103927 (+) 1035 WP_233436572.1 competence type IV pilus assembly protein ComGB Machinery gene
  JF649_RS00655 (JF649_00660) comYC 103929..104255 (+) 327 WP_002986552.1 competence type IV pilus major pilin ComGC Machinery gene
  JF649_RS00660 (JF649_00665) comGD 104230..104658 (+) 429 WP_002986548.1 competence type IV pilus minor pilin ComGD -
  JF649_RS00665 (JF649_00670) comGE 104615..104899 (+) 285 WP_032465969.1 competence type IV pilus minor pilin ComGE -
  JF649_RS00670 (JF649_00675) comYF 104892..105326 (+) 435 WP_129321637.1 competence type IV pilus minor pilin ComGF Machinery gene
  JF649_RS00675 (JF649_00680) comGG 105310..105636 (+) 327 WP_129321638.1 competence type IV pilus minor pilin ComGG -
  JF649_RS00680 (JF649_00685) comYH 105734..106687 (+) 954 WP_002987790.1 class I SAM-dependent methyltransferase Machinery gene
  JF649_RS00685 (JF649_00690) - 106746..107942 (+) 1197 WP_010921803.1 acetate kinase -

Sequence

Protein


Download         Length: 312 a.a.        Molecular weight: 35059.30 Da        Isoelectric Point: 8.5799

>NTDB_id=520666 JF649_RS00645 WP_063812149.1 102019..102957(+) (comYA) [Streptococcus pyogenes strain MGAS270]
MVQALAKAILAKAEQVQAQDIYILPRADQYDLFLRIGDERRLVDVYQSDRMAPLISHFKFVAGMIVGEKRRCQVGSCDYK
LSKDKQLSLRLSSVGDYRGQESLVIRLLHHQNKSVHYWFDGLTKVANQVGGRGLYLFAGPVGSGKTTLMYQLISNYHQEA
QVISIEDPVEIKNHQILQLQVNDDIGMTYDNLIKLSLRHRPDILVIGEIRDSQTARAVIRASLTGAMVFSTVHAKSISGV
YARLLELGVTKAELSNCLALIAYQRLLNGGALIDSTQNEFEYYSSSNWNQQIDQLLEAGHLNPKQAKLEKII

Nucleotide


Download         Length: 939 bp        

>NTDB_id=520666 JF649_RS00645 WP_063812149.1 102019..102957(+) (comYA) [Streptococcus pyogenes strain MGAS270]
ATGGTACAAGCATTAGCAAAAGCTATTCTAGCAAAAGCTGAACAGGTTCAGGCACAAGATATTTATATTTTGCCAAGAGC
AGATCAATATGATCTTTTTTTACGAATAGGAGATGAAAGGAGATTAGTTGATGTTTATCAGAGCGATCGGATGGCTCCTC
TTATTAGTCACTTTAAATTCGTTGCAGGAATGATAGTTGGTGAAAAGAGACGTTGTCAGGTGGGTTCATGTGATTATAAG
CTTAGTAAAGATAAGCAGTTATCTTTGCGCTTATCTAGCGTGGGTGATTATCGCGGGCAAGAAAGCTTAGTGATTCGTCT
GCTTCATCATCAAAATAAAAGTGTACATTATTGGTTTGATGGATTGACAAAAGTAGCCAATCAAGTTGGCGGTAGAGGGT
TGTATTTATTTGCAGGACCAGTTGGGTCTGGGAAGACAACCTTGATGTACCAGCTGATTTCGAATTATCATCAAGAAGCA
CAGGTTATTAGTATAGAAGATCCTGTAGAAATTAAAAATCACCAGATTTTACAATTACAAGTGAATGATGATATTGGTAT
GACTTATGACAATTTGATCAAACTGTCTTTACGCCATCGACCAGATATTTTAGTTATTGGTGAGATTCGAGATAGTCAAA
CAGCAAGAGCCGTTATTAGGGCTAGTCTAACAGGTGCCATGGTTTTTTCAACGGTTCACGCTAAAAGTATCTCGGGTGTT
TATGCAAGATTGTTAGAACTTGGTGTAACGAAAGCAGAACTGTCTAATTGCTTAGCATTAATTGCTTACCAAAGGTTACT
TAATGGAGGAGCATTGATTGACTCTACTCAAAACGAATTTGAATATTATTCCTCATCGAACTGGAATCAACAAATTGATC
AGCTTCTTGAGGCAGGACATCTCAATCCCAAGCAAGCTAAGCTTGAAAAAATTATCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-267)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comYA Streptococcus mutans UA140

61.736

99.679

0.615
  comYA Streptococcus mutans UA159

61.736

99.679

0.615
  comYA Streptococcus gordonii str. Challis substr. CH1

60.772

99.679

0.606
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

60.645

99.359

0.603
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

59.486

99.679

0.593
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

58.842

99.679

0.587
  comGA/cglA/cilD Streptococcus pneumoniae D39

58.842

99.679

0.587
  comGA/cglA/cilD Streptococcus pneumoniae R6

58.842

99.679

0.587
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

58.842

99.679

0.587
  comGA Lactococcus lactis subsp. cremoris KW2

50.482

99.679

0.503
  comGA Latilactobacillus sakei subsp. sakei 23K

39.655

92.949

0.369