Detailed information    

insolico Bioinformatically predicted

Overview

Name   comYF   Type   Machinery gene
Locus tag   JF649_RS00670 Genome accession   NZ_CP066541
Coordinates   104892..105326 (+) Length   144 a.a.
NCBI ID   WP_129321637.1    Uniprot ID   -
Organism   Streptococcus pyogenes strain MGAS270     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 99892..110326
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JF649_RS00640 (JF649_00645) - 101561..101926 (+) 366 WP_002986560.1 DUF1033 family protein -
  JF649_RS00645 (JF649_00650) comYA 102019..102957 (+) 939 WP_063812149.1 competence type IV pilus ATPase ComGA Machinery gene
  JF649_RS00650 (JF649_00655) comYB 102893..103927 (+) 1035 WP_233436572.1 competence type IV pilus assembly protein ComGB Machinery gene
  JF649_RS00655 (JF649_00660) comYC 103929..104255 (+) 327 WP_002986552.1 competence type IV pilus major pilin ComGC Machinery gene
  JF649_RS00660 (JF649_00665) comGD 104230..104658 (+) 429 WP_002986548.1 competence type IV pilus minor pilin ComGD -
  JF649_RS00665 (JF649_00670) comGE 104615..104899 (+) 285 WP_032465969.1 competence type IV pilus minor pilin ComGE -
  JF649_RS00670 (JF649_00675) comYF 104892..105326 (+) 435 WP_129321637.1 competence type IV pilus minor pilin ComGF Machinery gene
  JF649_RS00675 (JF649_00680) comGG 105310..105636 (+) 327 WP_129321638.1 competence type IV pilus minor pilin ComGG -
  JF649_RS00680 (JF649_00685) comYH 105734..106687 (+) 954 WP_002987790.1 class I SAM-dependent methyltransferase Machinery gene
  JF649_RS00685 (JF649_00690) - 106746..107942 (+) 1197 WP_010921803.1 acetate kinase -
  JF649_RS00690 (JF649_00695) - 108129..108437 (+) 309 WP_111677000.1 hypothetical protein -
  JF649_RS00695 (JF649_00700) proC 108520..109290 (-) 771 WP_129321639.1 pyrroline-5-carboxylate reductase -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 16587.23 Da        Isoelectric Point: 10.3584

>NTDB_id=520669 JF649_RS00670 WP_129321637.1 104892..105326(+) (comYF) [Streptococcus pyogenes strain MGAS270]
MSKQLSNIKAFTLLEALIALLVISGSLLVYQGLTQTLLKRSHYLARHDQDNWLLFSHQLREELSGARFYKVADNKLYVEK
GKKVLAFGQFKSHDFRKSASNGKGYQPMLFGISRSHIHIEQSQICITLKWKSGLERTFYYAFQG

Nucleotide


Download         Length: 435 bp        

>NTDB_id=520669 JF649_RS00670 WP_129321637.1 104892..105326(+) (comYF) [Streptococcus pyogenes strain MGAS270]
TTGAGTAAACAATTAAGTAATATAAAAGCTTTTACCCTTCTAGAGGCGTTAATAGCCTTACTCGTGATATCAGGGTCTTT
ATTGGTTTATCAAGGTTTGACCCAAACCCTCCTTAAACGTAGCCATTATCTAGCCCGTCATGATCAAGACAATTGGCTCT
TATTTTCTCATCAATTGCGAGAGGAGCTAAGTGGAGCAAGATTTTACAAAGTAGCTGATAATAAACTATACGTTGAAAAA
GGAAAGAAAGTACTAGCTTTTGGCCAATTTAAAAGTCATGATTTCCGAAAATCAGCTAGTAATGGAAAAGGGTATCAACC
CATGTTATTTGGAATATCACGTAGTCATATTCACATAGAGCAGTCACAGATTTGCATTACTTTAAAGTGGAAAAGTGGGT
TAGAAAGGACTTTTTATTATGCCTTTCAAGGCTAA

Domains

Predicted by InterproScan.

Putative Competence protein ComGF (ComGF)

(41-135)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comYF Streptococcus mutans UA140

51.825

95.139

0.493
  comYF Streptococcus mutans UA159

51.095

95.139

0.486
  comGF Lactococcus lactis subsp. cremoris KW2

46.667

93.75

0.438
  comGF/cglF Streptococcus mitis NCTC 12261

44.776

93.056

0.417
  comGF/cglF Streptococcus pneumoniae Rx1

43.609

92.361

0.403
  comGF/cglF Streptococcus pneumoniae D39

43.609

92.361

0.403
  comGF/cglF Streptococcus pneumoniae R6

43.609

92.361

0.403
  comGF/cglF Streptococcus pneumoniae TIGR4

43.609

92.361

0.403
  comGF/cglF Streptococcus mitis SK321

42.857

92.361

0.396