Detailed information    

insolico Bioinformatically predicted

Overview

Name   comYH   Type   Machinery gene
Locus tag   JF649_RS00680 Genome accession   NZ_CP066541
Coordinates   105734..106687 (+) Length   317 a.a.
NCBI ID   WP_002987790.1    Uniprot ID   A0A5S4TCX5
Organism   Streptococcus pyogenes strain MGAS270     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 100734..111687
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JF649_RS00640 (JF649_00645) - 101561..101926 (+) 366 WP_002986560.1 DUF1033 family protein -
  JF649_RS00645 (JF649_00650) comYA 102019..102957 (+) 939 WP_063812149.1 competence type IV pilus ATPase ComGA Machinery gene
  JF649_RS00650 (JF649_00655) comYB 102893..103927 (+) 1035 WP_233436572.1 competence type IV pilus assembly protein ComGB Machinery gene
  JF649_RS00655 (JF649_00660) comYC 103929..104255 (+) 327 WP_002986552.1 competence type IV pilus major pilin ComGC Machinery gene
  JF649_RS00660 (JF649_00665) comGD 104230..104658 (+) 429 WP_002986548.1 competence type IV pilus minor pilin ComGD -
  JF649_RS00665 (JF649_00670) comGE 104615..104899 (+) 285 WP_032465969.1 competence type IV pilus minor pilin ComGE -
  JF649_RS00670 (JF649_00675) comYF 104892..105326 (+) 435 WP_129321637.1 competence type IV pilus minor pilin ComGF Machinery gene
  JF649_RS00675 (JF649_00680) comGG 105310..105636 (+) 327 WP_129321638.1 competence type IV pilus minor pilin ComGG -
  JF649_RS00680 (JF649_00685) comYH 105734..106687 (+) 954 WP_002987790.1 class I SAM-dependent methyltransferase Machinery gene
  JF649_RS00685 (JF649_00690) - 106746..107942 (+) 1197 WP_010921803.1 acetate kinase -
  JF649_RS00690 (JF649_00695) - 108129..108437 (+) 309 WP_111677000.1 hypothetical protein -
  JF649_RS00695 (JF649_00700) proC 108520..109290 (-) 771 WP_129321639.1 pyrroline-5-carboxylate reductase -
  JF649_RS00700 (JF649_00705) pepA 109338..110405 (-) 1068 WP_115219557.1 glutamyl aminopeptidase -
  JF649_RS00705 (JF649_00710) - 110870..111154 (+) 285 WP_002992749.1 DUF4651 domain-containing protein -
  JF649_RS00710 (JF649_00715) - 111151..111468 (+) 318 WP_002986515.1 thioredoxin family protein -

Sequence

Protein


Download         Length: 317 a.a.        Molecular weight: 35985.08 Da        Isoelectric Point: 4.7205

>NTDB_id=520670 JF649_RS00680 WP_002987790.1 105734..106687(+) (comYH) [Streptococcus pyogenes strain MGAS270]
MTFEKIEEAYQLLLENCQLIENDLKTHIYDAIVEQNSFYLGAEGASPQVAQNSDKLKALCLTKEEWRKAYQFLFIKAAQT
EQLQANHQFTPDAIGFILLYLLEQLSDKDSLEVLEIGSGTGNLAQTLLNNTSKSLDYVGIELDDLLIDLSASIAEIMDSS
AHFIQEDAVRPQLLKESDIVISDLPVGYYPNDDIAKRYKVASSDKHTYAHHLLMEQSLKYLKKDGFAIFLAPVNLLTSPQ
SQLLKQWLKDYAQVVALITLPDSIFGHPSNAKSIIVLQKQTDHPMETFVYPIRDLKLAENIHDFMENFKKWKLSNVN

Nucleotide


Download         Length: 954 bp        

>NTDB_id=520670 JF649_RS00680 WP_002987790.1 105734..106687(+) (comYH) [Streptococcus pyogenes strain MGAS270]
ATGACTTTTGAAAAAATTGAAGAAGCTTATCAGCTGCTCTTAGAGAACTGTCAACTAATTGAAAATGATCTAAAAACACA
TATATACGATGCCATTGTTGAACAAAACTCCTTTTATTTAGGGGCTGAGGGAGCTAGTCCTCAAGTTGCTCAAAATAGTG
ATAAACTGAAAGCCTTGTGCCTGACAAAAGAAGAATGGCGTAAGGCCTACCAGTTTCTTTTTATTAAGGCAGCTCAGACG
GAGCAACTCCAAGCCAACCATCAGTTCACACCAGATGCTATTGGCTTCATTCTGCTGTATCTTTTGGAACAATTGAGTGA
TAAAGATAGCTTAGAGGTACTTGAGATTGGAAGTGGAACAGGGAACCTAGCCCAAACCCTTCTCAACAACACGAGCAAGA
GCCTTGATTATGTAGGGATTGAACTTGATGATCTCTTGATTGATCTGTCAGCCAGTATTGCTGAAATAATGGATTCTTCA
GCTCATTTTATTCAAGAAGATGCGGTAAGGCCTCAATTACTAAAAGAAAGTGACATTGTCATCAGTGACTTACCAGTTGG
TTATTATCCTAACGATGATATTGCCAAACGGTACAAGGTGGCTAGTTCAGATAAGCATACCTATGCCCATCATTTATTAA
TGGAACAGTCTTTAAAATACTTGAAAAAAGACGGTTTTGCGATTTTTCTGGCACCAGTCAATTTATTGACGAGCCCTCAG
AGCCAGTTATTGAAACAGTGGTTAAAAGATTATGCTCAGGTGGTGGCCTTGATTACGCTACCAGATTCTATTTTTGGTCA
TCCCTCAAATGCCAAGTCCATTATTGTCTTACAAAAACAAACAGACCACCCAATGGAAACCTTTGTCTATCCAATTCGGG
ATTTGAAGCTTGCAGAGAATATTCATGATTTTATGGAAAATTTCAAAAAATGGAAACTGAGTAATGTCAATTAA

Domains

Predicted by InterproScan.

N-6 DNA Methylase (N6_Mtase)

(68-288)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5S4TCX5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comYH Streptococcus mutans UA159

66.139

99.685

0.659
  comYH Streptococcus mutans UA140

65.823

99.685

0.656