Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   I9H09_RS02860 Genome accession   NZ_CP066270
Coordinates   641614..642831 (-) Length   405 a.a.
NCBI ID   WP_024669673.1    Uniprot ID   A0AB37QZB3
Organism   Pseudomonas tremae strain PA-1-10F     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 636614..647831
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I9H09_RS02830 (I9H09_02835) - 637095..638393 (-) 1299 WP_236502298.1 NAD(P)/FAD-dependent oxidoreductase -
  I9H09_RS02835 (I9H09_02840) - 638611..639144 (-) 534 WP_005895644.1 MOSC domain-containing protein -
  I9H09_RS02840 (I9H09_02845) - 639141..639770 (-) 630 WP_005895647.1 DUF1780 domain-containing protein -
  I9H09_RS02845 (I9H09_02850) yacG 639914..640123 (-) 210 WP_005895648.1 DNA gyrase inhibitor YacG -
  I9H09_RS02850 (I9H09_02855) coaE 640120..640743 (-) 624 WP_005895649.1 dephospho-CoA kinase -
  I9H09_RS02855 (I9H09_02860) pilD 640740..641612 (-) 873 WP_236502297.1 A24 family peptidase Machinery gene
  I9H09_RS02860 (I9H09_02865) pilC 641614..642831 (-) 1218 WP_024669673.1 type II secretion system F family protein Machinery gene
  I9H09_RS02865 (I9H09_02870) pilB 642834..644528 (-) 1695 WP_024669672.1 type IV-A pilus assembly ATPase PilB Machinery gene
  I9H09_RS02870 (I9H09_02875) - 644755..645189 (+) 435 WP_024669671.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  I9H09_RS02875 (I9H09_02880) - 645490..645822 (-) 333 WP_032618469.1 helix-turn-helix domain-containing protein -
  I9H09_RS02880 (I9H09_02885) gstA 645991..646611 (+) 621 WP_024669669.1 glutathione transferase GstA -
  I9H09_RS02885 (I9H09_02890) - 646710..647255 (-) 546 WP_024669668.1 DUF1523 family protein -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44121.07 Da        Isoelectric Point: 10.1493

>NTDB_id=519052 I9H09_RS02860 WP_024669673.1 641614..642831(-) (pilC) [Pseudomonas tremae strain PA-1-10F]
MASKAVKVSIYSWEGLDKKGGKLSGELSGHNPALIKAQLRKQGVNPTKVRKKSISIFGKGKKIKPLDIAFFSRQMATMMK
AGVPLLQSFDIISEGAENPNMRTLVVSLKQEVSAGNSFATALRQKPEYFDELFCNLVDAGEQAGALESLLDRVATYKEKT
EKLKAKIKKAMTYPTAVLVVAVIVSGILLIKVVPQFQTVFSSFGADLPLFTLMVIALSEFVQQWWLAIIGLLFGGFFLFK
RAYKQSQKFRDGLDRFLLKMPIIGPLIFKSSVARYARTLATTFAAGVPLVEALDSVAGATGNVVFRNAVHKVKQDVSTGM
QLNFSMRSTGVFPSLAIQMTAIGEESGALDAMLDKVASYYEDEVDNMVDSLTSLMEPMIMAVLGVIVGGLVIAMYLPIFK
LGSVV

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=519052 I9H09_RS02860 WP_024669673.1 641614..642831(-) (pilC) [Pseudomonas tremae strain PA-1-10F]
ATGGCCAGCAAGGCAGTCAAAGTCAGTATTTATAGCTGGGAAGGCCTGGATAAAAAAGGCGGAAAACTCAGTGGAGAGTT
GAGCGGTCACAATCCAGCTCTGATAAAAGCCCAACTGCGCAAGCAGGGCGTCAATCCGACCAAAGTACGCAAGAAATCAA
TTTCGATATTTGGAAAAGGCAAGAAAATCAAGCCGCTGGATATAGCTTTCTTCTCCCGGCAGATGGCCACCATGATGAAA
GCCGGAGTACCTTTACTCCAATCGTTCGACATAATTAGTGAAGGTGCAGAAAATCCCAATATGCGAACACTGGTGGTATC
GTTAAAGCAGGAAGTCTCAGCGGGTAACAGCTTTGCTACTGCCTTGAGACAAAAGCCTGAATATTTTGATGAGCTGTTCT
GCAACCTTGTCGATGCAGGTGAGCAGGCAGGCGCATTGGAAAGTCTTCTGGACAGGGTTGCAACGTACAAGGAAAAAACC
GAAAAGCTCAAGGCCAAGATCAAGAAAGCAATGACATATCCTACCGCTGTTCTGGTGGTGGCAGTTATAGTGTCAGGCAT
CTTGCTGATTAAGGTAGTACCGCAATTCCAGACAGTATTTTCAAGCTTCGGCGCGGATTTACCTCTATTCACCTTGATGG
TCATCGCTCTTTCAGAATTTGTGCAGCAATGGTGGCTAGCTATTATCGGCTTGCTCTTTGGAGGTTTTTTTCTGTTCAAG
CGGGCCTACAAACAGTCTCAGAAATTCCGCGACGGTTTGGACAGGTTTTTGTTAAAAATGCCGATCATAGGCCCGCTAAT
TTTCAAATCATCAGTAGCCCGCTATGCGCGCACACTTGCAACGACTTTCGCTGCCGGTGTTCCCTTGGTCGAGGCACTGG
ACTCGGTTGCTGGGGCAACTGGGAATGTGGTGTTCCGAAATGCAGTCCACAAAGTAAAGCAGGATGTCTCTACAGGCATG
CAATTGAATTTCTCGATGCGCTCTACAGGTGTATTCCCAAGTTTAGCGATCCAGATGACAGCCATTGGTGAGGAATCCGG
CGCCTTGGACGCAATGCTGGACAAGGTCGCTAGCTACTACGAGGATGAAGTCGACAACATGGTTGATAGCCTAACCAGTC
TAATGGAACCAATGATCATGGCTGTACTGGGCGTTATCGTCGGCGGCTTAGTCATCGCTATGTACCTGCCCATATTCAAA
CTCGGAAGCGTCGTCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(276-397)

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

73.58

100

0.736
  pilC Acinetobacter baylyi ADP1

59.804

100

0.602
  pilC Acinetobacter baumannii D1279779

59.901

99.753

0.598
  pilC Legionella pneumophila strain ERS1305867

53.283

97.778

0.521
  pilG Neisseria gonorrhoeae MS11

42.68

99.506

0.425
  pilG Neisseria meningitidis 44/76-A

42.432

99.506

0.422
  pilC Vibrio campbellii strain DS40M4

41.463

100

0.42
  pilC Vibrio cholerae strain A1552

40.247

100

0.402
  pilC Thermus thermophilus HB27

38.903

99.012

0.385