Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   I9H09_RS02865 Genome accession   NZ_CP066270
Coordinates   642834..644528 (-) Length   564 a.a.
NCBI ID   WP_024669672.1    Uniprot ID   -
Organism   Pseudomonas tremae strain PA-1-10F     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 637834..649528
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I9H09_RS02835 (I9H09_02840) - 638611..639144 (-) 534 WP_005895644.1 MOSC domain-containing protein -
  I9H09_RS02840 (I9H09_02845) - 639141..639770 (-) 630 WP_005895647.1 DUF1780 domain-containing protein -
  I9H09_RS02845 (I9H09_02850) yacG 639914..640123 (-) 210 WP_005895648.1 DNA gyrase inhibitor YacG -
  I9H09_RS02850 (I9H09_02855) coaE 640120..640743 (-) 624 WP_005895649.1 dephospho-CoA kinase -
  I9H09_RS02855 (I9H09_02860) pilD 640740..641612 (-) 873 WP_236502297.1 A24 family peptidase Machinery gene
  I9H09_RS02860 (I9H09_02865) pilC 641614..642831 (-) 1218 WP_024669673.1 type II secretion system F family protein Machinery gene
  I9H09_RS02865 (I9H09_02870) pilB 642834..644528 (-) 1695 WP_024669672.1 type IV-A pilus assembly ATPase PilB Machinery gene
  I9H09_RS02870 (I9H09_02875) - 644755..645189 (+) 435 WP_024669671.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  I9H09_RS02875 (I9H09_02880) - 645490..645822 (-) 333 WP_032618469.1 helix-turn-helix domain-containing protein -
  I9H09_RS02880 (I9H09_02885) gstA 645991..646611 (+) 621 WP_024669669.1 glutathione transferase GstA -
  I9H09_RS02885 (I9H09_02890) - 646710..647255 (-) 546 WP_024669668.1 DUF1523 family protein -
  I9H09_RS02890 (I9H09_02895) - 647375..648373 (+) 999 WP_024669667.1 ATP-grasp domain-containing protein -

Sequence

Protein


Download         Length: 564 a.a.        Molecular weight: 62534.84 Da        Isoelectric Point: 6.3341

>NTDB_id=519053 I9H09_RS02865 WP_024669672.1 642834..644528(-) (pilB) [Pseudomonas tremae strain PA-1-10F]
MTDVVLTGLAKQLVLAELLDEKTAQQAYLQARRDKLSLVSYLVQNKLVKSLTLAEMASDQFGIPFLDLANLDKESQPKGL
VSEKLVRQHHALPLWRRGNKLFIGISDPTNHQAVTDIQFSTGLNTEAILVADDKLSDAIEKFFDTGSGLGEMEDVDLGVD
IDHAEGKETSLADQSDADDAPVVRFVNKMLMDAIRIGSSDLHFEPYEKIFRVRLRTDGILHEVARPPIHLANRIAARLKV
MASLDISERRKPQDGRVKLRVSKTKAIDFRMNTLPTLWGEKIVMRILDPTSAQMGIDALGYEPEQKALYLEALKQPQGMI
LVTGPTGSGKTVSLYTGLNILNTVDINISTAEDPVEINLEGINQVNVNPRQGLDFSQALRAFLRQDPDVIMVGEIRDLET
AEIAIKASQTGHMVLSTLHTNSAAETLTRLHHMGVAAFNIATAINLIIAQRLARKLCSHCKKELDIPRETLIREGFPEAK
IGTFKIYGPVGCEHCNGGYRGRVGIYEVVKKTPELERIIMEEGNSLEISRQMRKDGFNDLRTSGLSKSMQGITSLEEVNR
VTKD

Nucleotide


Download         Length: 1695 bp        

>NTDB_id=519053 I9H09_RS02865 WP_024669672.1 642834..644528(-) (pilB) [Pseudomonas tremae strain PA-1-10F]
ATGACTGATGTTGTCCTCACTGGTTTGGCCAAACAACTGGTTCTGGCCGAGCTTCTCGACGAAAAAACGGCTCAGCAAGC
TTACCTGCAAGCACGCCGCGACAAGCTTTCGCTGGTTAGCTATTTAGTTCAGAACAAACTGGTCAAAAGTCTGACGCTTG
CAGAGATGGCGTCTGACCAGTTCGGCATTCCGTTTTTAGACCTTGCAAATCTGGACAAGGAGAGTCAGCCCAAAGGCTTG
GTCAGTGAAAAACTGGTCCGGCAGCACCATGCTCTCCCCCTTTGGCGGCGAGGCAACAAGCTTTTTATTGGTATATCTGA
CCCCACAAACCATCAAGCAGTCACTGATATCCAGTTCAGTACAGGTCTGAACACTGAGGCGATATTGGTCGCCGACGACA
AGCTTAGCGATGCAATCGAAAAATTTTTCGATACTGGCAGTGGCCTCGGAGAGATGGAGGATGTTGACCTTGGAGTGGAC
ATTGATCACGCAGAAGGCAAAGAGACATCGCTTGCTGACCAAAGCGACGCCGATGACGCTCCAGTAGTGCGCTTCGTCAA
TAAAATGCTCATGGACGCTATCCGCATCGGCTCATCAGACCTGCACTTCGAGCCCTACGAAAAGATCTTTCGCGTGCGCT
TGCGTACCGATGGCATTTTGCATGAGGTTGCCAGGCCGCCGATTCATCTGGCAAACCGTATTGCTGCACGCCTGAAAGTC
ATGGCCAGCCTCGACATTTCGGAACGGCGCAAACCACAGGACGGCCGGGTCAAGTTGCGGGTGTCCAAAACCAAAGCGAT
TGATTTTCGTATGAATACCTTGCCCACACTGTGGGGCGAGAAAATCGTGATGCGTATTCTTGATCCGACCAGTGCGCAGA
TGGGCATTGACGCTCTGGGCTATGAACCAGAGCAAAAGGCACTGTATCTGGAGGCGCTAAAGCAGCCACAAGGCATGATT
CTTGTGACCGGCCCTACGGGTTCTGGCAAGACTGTTTCCCTGTATACCGGGCTGAATATCCTCAATACTGTGGACATCAA
CATTTCCACCGCTGAAGACCCGGTAGAGATCAACCTTGAAGGCATCAATCAGGTCAACGTCAACCCGCGTCAGGGGCTGG
ACTTTTCTCAGGCATTGCGCGCCTTTCTGCGTCAAGATCCCGACGTAATCATGGTTGGTGAGATTCGCGACCTGGAAACA
GCGGAAATAGCCATCAAGGCCTCCCAAACCGGGCATATGGTGCTGTCTACGCTTCACACCAACAGCGCGGCAGAAACCTT
GACCCGACTGCATCACATGGGTGTCGCAGCATTCAACATTGCTACAGCAATCAACCTGATTATTGCGCAACGGCTTGCGC
GCAAGTTGTGCAGCCACTGCAAGAAAGAGCTCGATATTCCTCGTGAAACGCTGATTAGAGAAGGGTTTCCAGAAGCGAAA
ATTGGCACGTTCAAAATCTACGGCCCTGTGGGTTGTGAGCATTGCAATGGCGGTTATAGAGGAAGGGTCGGTATTTACGA
GGTGGTCAAAAAGACGCCAGAGCTAGAACGGATCATCATGGAAGAAGGTAACTCGCTCGAGATTTCCAGGCAGATGCGCA
AGGACGGCTTCAATGATCTGCGTACCTCAGGTCTTTCAAAGTCTATGCAAGGCATCACCAGCCTAGAAGAAGTCAACCGT
GTGACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(188-455)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-146)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

58.007

99.645

0.578
  pilB Acinetobacter baylyi ADP1

57.295

99.645

0.571
  pilB Legionella pneumophila strain ERS1305867

55.282

100

0.557
  pilB Vibrio cholerae strain A1552

50.265

100

0.504
  pilB Vibrio parahaemolyticus RIMD 2210633

51.201

95.922

0.491
  pilB Vibrio campbellii strain DS40M4

49.113

100

0.491
  pilF Neisseria gonorrhoeae MS11

48.043

99.645

0.479
  pilF Thermus thermophilus HB27

38.516

100

0.387
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.122

92.908

0.363