Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   I9H07_RS19970 Genome accession   NZ_CP066265
Coordinates   4476758..4477975 (+) Length   405 a.a.
NCBI ID   WP_024674281.1    Uniprot ID   -
Organism   Pseudomonas syringae strain PA-2-9E     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4471758..4482975
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I9H07_RS19945 (I9H07_19825) - 4472327..4472872 (+) 546 WP_058391476.1 DUF1523 family protein -
  I9H07_RS19950 (I9H07_19830) gstA 4472969..4473589 (-) 621 WP_236423973.1 glutathione transferase GstA -
  I9H07_RS19955 (I9H07_19835) - 4473745..4474098 (+) 354 WP_236423972.1 helix-turn-helix domain-containing protein -
  I9H07_RS19960 (I9H07_19840) - 4474399..4474833 (-) 435 WP_236423970.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  I9H07_RS19965 (I9H07_19845) pilB 4475061..4476755 (+) 1695 WP_024674282.1 type IV-A pilus assembly ATPase PilB Machinery gene
  I9H07_RS19970 (I9H07_19850) pilC 4476758..4477975 (+) 1218 WP_024674281.1 type II secretion system F family protein Machinery gene
  I9H07_RS19975 (I9H07_19855) pilD 4477977..4478849 (+) 873 WP_236423968.1 A24 family peptidase Machinery gene
  I9H07_RS19980 (I9H07_19860) coaE 4478846..4479469 (+) 624 WP_236423966.1 dephospho-CoA kinase -
  I9H07_RS19985 (I9H07_19865) yacG 4479466..4479675 (+) 210 WP_007251656.1 DNA gyrase inhibitor YacG -
  I9H07_RS19990 (I9H07_19870) - 4479973..4480299 (-) 327 WP_236423964.1 hypothetical protein -
  I9H07_RS19995 (I9H07_19875) - 4480497..4481126 (+) 630 WP_024645221.1 DUF1780 domain-containing protein -
  I9H07_RS20000 (I9H07_19880) - 4481123..4481656 (+) 534 WP_024673693.1 MOSC domain-containing protein -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44009.87 Da        Isoelectric Point: 10.1809

>NTDB_id=519008 I9H07_RS19970 WP_024674281.1 4476758..4477975(+) (pilC) [Pseudomonas syringae strain PA-2-9E]
MASKAVKVSIYTWEGLDKKGGKLSGELSGHNPALIKAQLRKQGVNPTKVRKKSTSIFGNGKKIKPLDIAFFSRQMATMMK
AGVPLLQSFDIISEGAENPNMRTLVVSLKQEVSAGNSFATALRQKPEHFDELFCNLVDAGEQAGALESLLDRVATYKEKT
EKLKAKIKKAMTYPTAVLVVAVIVSGILLIKVVPQFQTVFSSFGADLPLFTLMVIALSEFVQQWWLAILGLLVGGFFLFK
RAYKKSQKFRDGLDRFLLKVPIIGPLIFKSSVARYARTLATTFAAGVPLVEALDSVAGATGNVVFRNAVNKVKQDVSTGM
QLNFSMRSTGVFPSLAIQMTAIGEESGALDTMLDKVASYYEDEVDNMVDSLTSLMEPMIMAVLGVIVGGLVIAMYLPIFK
LGSVV

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=519008 I9H07_RS19970 WP_024674281.1 4476758..4477975(+) (pilC) [Pseudomonas syringae strain PA-2-9E]
ATGGCCAGCAAGGCAGTTAAAGTCAGTATCTATACTTGGGAAGGCTTGGACAAAAAAGGTGGAAAGCTCAGTGGAGAATT
GAGCGGGCACAATCCAGCACTTATAAAAGCCCAACTGCGCAAGCAGGGCGTCAACCCGACCAAAGTACGCAAAAAGTCAA
CCTCTATCTTTGGAAACGGCAAGAAAATCAAGCCATTAGATATAGCCTTTTTCTCTCGACAGATGGCTACCATGATGAAG
GCTGGCGTACCTCTGCTCCAGTCGTTCGACATAATCAGCGAGGGCGCCGAAAACCCCAACATGCGAACGTTGGTGGTGTC
GCTAAAACAGGAAGTCTCTGCGGGTAACAGTTTCGCAACAGCCTTGCGACAAAAGCCGGAGCATTTCGATGAGCTGTTCT
GCAACCTCGTCGATGCAGGTGAGCAGGCCGGTGCGTTGGAAAGCCTTTTAGACAGGGTTGCAACCTACAAGGAAAAAACT
GAAAAGCTCAAAGCCAAGATCAAGAAAGCCATGACATACCCTACTGCGGTTTTAGTGGTGGCGGTTATAGTTTCAGGAAT
TCTGCTAATCAAAGTGGTACCGCAATTTCAGACAGTATTTTCAAGTTTCGGCGCAGATTTACCCCTATTCACCCTTATGG
TGATTGCCCTTTCAGAGTTTGTGCAGCAATGGTGGCTAGCTATTCTCGGCCTGCTCGTTGGAGGCTTTTTTCTGTTCAAA
CGGGCCTACAAAAAATCTCAAAAATTTCGCGACGGCCTTGACAGGTTTTTGTTGAAAGTCCCAATCATAGGACCGCTGAT
TTTCAAGTCATCAGTAGCCCGCTATGCACGAACCCTTGCAACCACCTTCGCCGCTGGCGTGCCCTTGGTGGAGGCGCTGG
ACTCGGTTGCCGGGGCGACAGGAAATGTGGTGTTCAGAAATGCGGTTAATAAAGTAAAGCAGGATGTCTCCACTGGCATG
CAATTGAATTTCTCGATGCGCTCCACAGGTGTATTCCCAAGCTTAGCGATCCAGATGACAGCCATTGGTGAGGAGTCAGG
CGCTTTGGACACCATGCTGGACAAGGTCGCTAGCTACTACGAAGATGAAGTCGACAACATGGTTGATAGTCTAACCAGCC
TTATGGAGCCAATGATAATGGCGGTGCTGGGCGTTATCGTCGGCGGCTTGGTCATTGCCATGTACCTTCCCATCTTCAAA
CTTGGAAGCGTCGTCTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(276-397)

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

74.321

100

0.743
  pilC Acinetobacter baylyi ADP1

59.367

100

0.602
  pilC Acinetobacter baumannii D1279779

59.901

99.753

0.598
  pilC Legionella pneumophila strain ERS1305867

52.778

97.778

0.516
  pilG Neisseria gonorrhoeae MS11

42.469

100

0.425
  pilC Vibrio campbellii strain DS40M4

41.463

100

0.42
  pilG Neisseria meningitidis 44/76-A

41.728

100

0.417
  pilC Vibrio cholerae strain A1552

40.741

100

0.407
  pilC Thermus thermophilus HB27

38.903

99.012

0.385