Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   I9H07_RS19965 Genome accession   NZ_CP066265
Coordinates   4475061..4476755 (+) Length   564 a.a.
NCBI ID   WP_024674282.1    Uniprot ID   A0A9Q4A4W6
Organism   Pseudomonas syringae strain PA-2-9E     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4470061..4481755
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I9H07_RS19940 (I9H07_19820) - 4471208..4472206 (-) 999 WP_236423975.1 ATP-grasp domain-containing protein -
  I9H07_RS19945 (I9H07_19825) - 4472327..4472872 (+) 546 WP_058391476.1 DUF1523 family protein -
  I9H07_RS19950 (I9H07_19830) gstA 4472969..4473589 (-) 621 WP_236423973.1 glutathione transferase GstA -
  I9H07_RS19955 (I9H07_19835) - 4473745..4474098 (+) 354 WP_236423972.1 helix-turn-helix domain-containing protein -
  I9H07_RS19960 (I9H07_19840) - 4474399..4474833 (-) 435 WP_236423970.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  I9H07_RS19965 (I9H07_19845) pilB 4475061..4476755 (+) 1695 WP_024674282.1 type IV-A pilus assembly ATPase PilB Machinery gene
  I9H07_RS19970 (I9H07_19850) pilC 4476758..4477975 (+) 1218 WP_024674281.1 type II secretion system F family protein Machinery gene
  I9H07_RS19975 (I9H07_19855) pilD 4477977..4478849 (+) 873 WP_236423968.1 A24 family peptidase Machinery gene
  I9H07_RS19980 (I9H07_19860) coaE 4478846..4479469 (+) 624 WP_236423966.1 dephospho-CoA kinase -
  I9H07_RS19985 (I9H07_19865) yacG 4479466..4479675 (+) 210 WP_007251656.1 DNA gyrase inhibitor YacG -
  I9H07_RS19990 (I9H07_19870) - 4479973..4480299 (-) 327 WP_236423964.1 hypothetical protein -
  I9H07_RS19995 (I9H07_19875) - 4480497..4481126 (+) 630 WP_024645221.1 DUF1780 domain-containing protein -
  I9H07_RS20000 (I9H07_19880) - 4481123..4481656 (+) 534 WP_024673693.1 MOSC domain-containing protein -

Sequence

Protein


Download         Length: 564 a.a.        Molecular weight: 62603.87 Da        Isoelectric Point: 6.3861

>NTDB_id=519007 I9H07_RS19965 WP_024674282.1 4475061..4476755(+) (pilB) [Pseudomonas syringae strain PA-2-9E]
MTDVVLTGLAKQLVLAELINEKNAQLAYQQARRDKISLVSYLVQNKLVKSLTLAEMASDQFGIPFMDLSNLDKESQPKGL
VSEKLVRQHHALPLWRRGNKLFIGISDPTNHQAVTDIQFSTGLNTEAILVEDDKLSNAIDKFFETGNGLGEMEDVDLGLD
VDQSDSKETSLADQSDADDAPVVRFVNKMLMDAIRLGSSDLHFEPYEKTFRVRLRTDGILHEVAKPPIHLANRIAARLKV
MASLDISERRKPQDGRVKLRVSKTKAIDFRMNTLPTLWGEKIVMRILDPTSAQMGIDALGYEPEQKALYLEALKQPQGMI
LVTGPTGSGKTVSLYTGLNILNTVDINISTAEDPVEINLEGINQVNVNPRQGLDFSQALRAFLRQDPDVIMVGEIRDLET
AEIAIKASQTGHMVLSTLHTNSAAETLTRLHHMGVAAFNIATAINLIIAQRLARKLCSHCKKELDIPRETLIKEGFPEAK
IGTFKIYGPVGCEHCNGGYRGRVGIYEVVKKTPELERIIMEEGNSLEISRQMRKDGFNDLRTSGLSKAMQGITSLEEVNR
VTKD

Nucleotide


Download         Length: 1695 bp        

>NTDB_id=519007 I9H07_RS19965 WP_024674282.1 4475061..4476755(+) (pilB) [Pseudomonas syringae strain PA-2-9E]
ATGACCGATGTCGTGCTCACGGGTTTGGCCAAGCAACTGGTTCTGGCCGAGCTTATCAACGAAAAAAATGCCCAACTGGC
CTATCAGCAGGCGCGACGCGACAAGATTTCGCTCGTCAGCTATCTGGTACAGAACAAGTTAGTCAAAAGCCTGACGCTTG
CGGAGATGGCGTCTGACCAATTCGGCATTCCGTTCATGGACCTGTCGAACCTTGATAAGGAAAGTCAGCCCAAAGGGCTG
GTAAGCGAAAAGCTCGTCCGGCAACACCATGCACTACCCCTGTGGCGACGCGGGAACAAGCTTTTTATCGGCATATCCGA
CCCTACTAATCATCAGGCAGTGACTGATATTCAGTTCAGCACCGGTTTAAATACCGAGGCGATACTCGTAGAGGACGACA
AACTCAGTAATGCCATCGATAAGTTTTTTGAGACGGGTAATGGCTTGGGAGAGATGGAAGACGTCGATCTCGGTCTCGAC
GTTGACCAGTCCGATAGTAAAGAGACCTCGCTTGCTGACCAAAGTGATGCTGACGACGCTCCGGTAGTACGTTTCGTCAA
TAAAATGTTGATGGACGCTATTCGACTCGGCTCCTCGGACCTCCATTTTGAGCCCTACGAAAAGACCTTTCGCGTACGCC
TGCGTACCGACGGGATTCTGCATGAAGTCGCCAAGCCTCCGATCCATCTGGCAAACCGTATTGCAGCGCGTTTAAAAGTT
ATGGCCAGCCTTGATATATCTGAGCGGCGCAAACCGCAGGATGGTCGAGTCAAGTTGCGGGTTTCCAAAACCAAAGCTAT
CGATTTTCGTATGAACACCTTGCCTACGCTGTGGGGCGAGAAGATCGTGATGCGGATCCTTGATCCGACCAGTGCGCAAA
TGGGCATTGATGCTCTGGGTTACGAGCCAGAACAAAAGGCATTGTATCTGGAGGCCCTTAAACAACCGCAAGGCATGATT
CTGGTCACAGGCCCTACAGGTTCGGGAAAGACTGTTTCGCTTTATACCGGCCTGAATATTCTCAATACTGTGGATATCAA
TATTTCCACCGCTGAGGACCCCGTAGAGATCAATCTGGAGGGCATCAATCAGGTCAACGTCAATCCGCGTCAAGGTCTGG
ATTTTTCACAAGCACTGCGTGCTTTTCTGCGTCAGGATCCCGACGTGATCATGGTCGGTGAGATCCGTGATTTGGAAACC
GCCGAAATCGCCATCAAGGCGTCGCAGACAGGCCATATGGTGCTGTCCACCTTGCACACCAACAGCGCCGCAGAAACTCT
GACCCGCCTGCATCACATGGGCGTCGCAGCGTTCAATATTGCTACGGCGATCAATCTGATTATCGCGCAACGACTCGCAC
GCAAGTTATGCAGCCATTGCAAGAAAGAACTCGATATTCCTCGCGAGACACTGATTAAGGAAGGCTTTCCAGAGGCGAAG
ATTGGCACTTTCAAGATATATGGTCCAGTGGGCTGCGAGCATTGCAACGGCGGCTACAGGGGCAGGGTCGGTATTTACGA
AGTGGTCAAAAAGACACCGGAGCTGGAACGTATCATTATGGAGGAAGGCAACTCGCTGGAGATATCCAGGCAGATGCGCA
AGGACGGCTTTAATGACCTGCGTACCTCGGGCCTGTCAAAGGCCATGCAGGGCATCACCAGCCTGGAAGAAGTCAACCGA
GTGACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-145)

Type II/IV secretion system protein (T2SSE)

(188-455)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

57.699

100

0.578
  pilB Acinetobacter baylyi ADP1

57.651

99.645

0.574
  pilB Legionella pneumophila strain ERS1305867

55.752

100

0.559
  pilB Vibrio cholerae strain A1552

51.423

99.645

0.512
  pilB Vibrio parahaemolyticus RIMD 2210633

51.476

96.099

0.495
  pilB Vibrio campbellii strain DS40M4

48.936

100

0.489
  pilF Neisseria gonorrhoeae MS11

48.221

99.645

0.481
  pilF Thermus thermophilus HB27

39.153

100

0.394