Detailed information NTG0519009

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: I9H07_RS19975
• Genome accession: NZ_CP066265
• Coordinates: 4477977..4478849 (+)
• Length: 290 a.a.
• NCBI ID: WP_236423968.1
• Uniprot ID: A0A9Q4A8B3
• Organism: Pseudomonas syringae strain PA-2-9E
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4472977..4483849

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
I9H07_RS19955 (I9H07_19835)	-	4473745..4474098 (+)	354	WP_236423972.1	helix-turn-helix domain-containing protein	-
I9H07_RS19960 (I9H07_19840)	-	4474399..4474833 (-)	435	WP_236423970.1	prepilin-type N-terminal cleavage/methylation domain-containing protein	-
I9H07_RS19965 (I9H07_19845)	pilB	4475061..4476755 (+)	1695	WP_024674282.1	type IV-A pilus assembly ATPase PilB	Machinery gene
I9H07_RS19970 (I9H07_19850)	pilC	4476758..4477975 (+)	1218	WP_024674281.1	type II secretion system F family protein	Machinery gene
I9H07_RS19975 (I9H07_19855)	pilD	4477977..4478849 (+)	873	WP_236423968.1	A24 family peptidase	Machinery gene
I9H07_RS19980 (I9H07_19860)	coaE	4478846..4479469 (+)	624	WP_236423966.1	dephospho-CoA kinase	-
I9H07_RS19985 (I9H07_19865)	yacG	4479466..4479675 (+)	210	WP_007251656.1	DNA gyrase inhibitor YacG	-
I9H07_RS19990 (I9H07_19870)	-	4479973..4480299 (-)	327	WP_236423964.1	hypothetical protein	-
I9H07_RS19995 (I9H07_19875)	-	4480497..4481126 (+)	630	WP_024645221.1	DUF1780 domain-containing protein	-
I9H07_RS20000 (I9H07_19880)	-	4481123..4481656 (+)	534	WP_024673693.1	MOSC domain-containing protein	-
I9H07_RS20005 (I9H07_19885)	-	4481871..4483169 (+)	1299	WP_024645223.1	NAD(P)/FAD-dependent oxidoreductase	-

Sequence

Protein

• Length: 290 a.a.
• Molecular weight: 32046.31 Da
• Isoelectric Point: 8.1651

>NTDB_id=519009 I9H07_RS19975 WP_236423968.1 4477977..4478849(+) (pilD) [Pseudomonas syringae strain PA-2-9E]
MPLLDLLASSPLAFVTTCCILGLIVGSFLNVVVYRLPKMMERDWKTQSREMLGLPVEPDQPVFNLLLPHSSCPHCAHKIR
PWENLPIISYVLLRGKCSHCKAPISKRYPLVELTCAVLSAYIAWHFGFGWQAAAMLVLSWGLLAMSLIDADHQLLPDSLV
LPLLWLGLIVNALGLFTSLSDALWGAVAGYLTLWSVYWLFKLITGKEGMGHGDFKLLAMLGAWGGWQILPLTILLSSLVG
AVLGVIMMRVRRLESGTPIPFGPYLAIAGWIALLWGGQINDSYMQFAGFR

Nucleotide

• Length: 873 bp

>NTDB_id=519009 I9H07_RS19975 WP_236423968.1 4477977..4478849(+) (pilD) [Pseudomonas syringae strain PA-2-9E]
ATGCCCCTCCTCGACCTCCTGGCCAGCTCGCCCCTGGCCTTCGTCACCACCTGCTGCATACTCGGCCTTATCGTCGGAAG
CTTCCTCAACGTAGTCGTCTACCGCCTGCCAAAAATGATGGAGCGCGACTGGAAAACCCAATCCCGTGAAATGCTCGGCC
TCCCCGTCGAACCGGACCAACCCGTCTTCAACCTGCTCCTCCCCCACTCCAGTTGCCCGCACTGCGCGCACAAGATCCGC
CCGTGGGAAAACCTGCCGATCATCAGCTACGTGCTGCTGCGCGGAAAATGCTCGCACTGCAAAGCCCCCATCAGCAAACG
CTACCCGCTGGTCGAGCTGACCTGCGCAGTGTTGTCGGCCTACATCGCGTGGCACTTCGGCTTCGGCTGGCAAGCGGCGG
CAATGCTGGTGTTGAGCTGGGGCCTGTTGGCGATGAGCCTGATTGATGCTGATCACCAACTACTGCCTGACTCGCTGGTG
CTGCCGCTGCTATGGCTGGGCCTGATCGTCAACGCTCTCGGGCTGTTCACCTCGCTGAGCGATGCGCTGTGGGGCGCAGT
AGCAGGTTATCTGACGCTGTGGTCGGTGTATTGGCTGTTCAAGCTGATCACAGGCAAGGAAGGCATGGGCCATGGCGACT
TCAAATTGCTGGCCATGCTTGGCGCGTGGGGCGGCTGGCAGATTTTGCCGCTGACGATCCTGTTGTCATCGCTGGTGGGC
GCGGTATTGGGAGTGATCATGATGCGTGTGCGCCGGCTTGAGAGCGGCACGCCGATCCCCTTTGGTCCTTATCTGGCCAT
TGCGGGGTGGATCGCTTTGCTCTGGGGTGGTCAAATAAACGACTCTTACATGCAGTTTGCCGGTTTCAGATGA

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (136-245)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (20-126)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	54.77	97.586	0.534
pilD	Vibrio campbellii strain DS40M4	52.297	97.586	0.51
pilD	Neisseria gonorrhoeae MS11	50.179	96.207	0.483
pilD	Acinetobacter baumannii D1279779	50.181	95.517	0.479
pilD	Acinetobacter nosocomialis M2	49.819	95.517	0.476