Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   I9H08_RS03755 Genome accession   NZ_CP066263
Coordinates   865984..867201 (-) Length   405 a.a.
NCBI ID   WP_103360220.1    Uniprot ID   -
Organism   Pseudomonas syringae pv. syringae strain Pss9644     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 860984..872201
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I9H08_RS03735 (I9H08_03710) - 863696..864157 (+) 462 WP_058445857.1 hypothetical protein -
  I9H08_RS03740 (I9H08_03715) yacG 864284..864493 (-) 210 WP_002552020.1 DNA gyrase inhibitor YacG -
  I9H08_RS03745 (I9H08_03720) coaE 864490..865113 (-) 624 WP_074909035.1 dephospho-CoA kinase -
  I9H08_RS03750 (I9H08_03725) pilD 865110..865982 (-) 873 WP_103360221.1 A24 family peptidase Machinery gene
  I9H08_RS03755 (I9H08_03730) pilC 865984..867201 (-) 1218 WP_103360220.1 type II secretion system F family protein Machinery gene
  I9H08_RS03760 (I9H08_03735) pilB 867204..868898 (-) 1695 WP_024684410.1 type IV-A pilus assembly ATPase PilB Machinery gene
  I9H08_RS03765 (I9H08_03740) - 869124..869519 (+) 396 WP_103360219.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  I9H08_RS03770 (I9H08_03745) - 869591..870988 (+) 1398 WP_103360218.1 O-antigen ligase family protein -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44238.12 Da        Isoelectric Point: 10.1095

>NTDB_id=518968 I9H08_RS03755 WP_103360220.1 865984..867201(-) (pilC) [Pseudomonas syringae pv. syringae strain Pss9644]
MASKAVKVSVYTWEGVDKKGGKLSGEVTGHNLALVKAQLRKQGINFTKVRKKPVSIFGKGKKIKPLDIAFFSRQMATMMK
AGVPLLQSFDIISEGAENPNMRALVGSLKQEVSAGNSFATALRKKPEYFDDLFCNLVDAGEQAGALESLLDRVASYKEKT
ERLKAKIKKAMTYPTAVLIVAFIVSGILLIKVVPQFQSVFAGFNAELPAFTLMVIALSEVVQQWWLAIVALFFVSFFMFK
RAYKQSQNFRDSLDRFLLKVPIIGPLIFKSSVARYARTLATTFAAGVPLVEALDSVAGATGNVVFRNAVNQVKQDVSTGM
QLNFSMRSTGVFPSLAIQMTAIGEESGALDAMLDKVATYYEEEVDNMVDNMTSLMEPMIMAVLGVIVGGLVIAMYLPIFK
LGSVV

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=518968 I9H08_RS03755 WP_103360220.1 865984..867201(-) (pilC) [Pseudomonas syringae pv. syringae strain Pss9644]
ATGGCCAGCAAGGCAGTTAAAGTCAGCGTCTACACGTGGGAAGGTGTGGACAAGAAAGGCGGCAAACTCAGTGGAGAAGT
GACAGGGCACAATCTCGCGCTCGTTAAGGCTCAGTTGCGCAAACAAGGCATCAATTTCACCAAGGTACGTAAAAAGCCCG
TTTCCATCTTTGGTAAAGGCAAGAAGATCAAGCCGCTGGACATCGCATTCTTTTCTCGCCAGATGGCAACCATGATGAAG
GCAGGTGTGCCCCTGCTGCAGTCATTTGACATCATTTCCGAGGGCGCAGAAAACCCGAACATGCGTGCGCTCGTCGGTTC
ACTAAAGCAGGAGGTTTCAGCAGGGAACAGCTTTGCGACCGCTCTGCGAAAGAAACCGGAATACTTCGACGACCTGTTCT
GCAACCTGGTCGATGCCGGCGAGCAAGCGGGTGCACTAGAGAGCCTGTTGGATCGCGTGGCGAGCTACAAGGAAAAAACG
GAAAGGCTAAAGGCCAAAATCAAGAAGGCGATGACTTATCCTACCGCGGTATTAATTGTCGCCTTTATCGTGTCAGGTAT
TTTGTTGATTAAGGTCGTGCCGCAGTTCCAGTCTGTCTTCGCTGGGTTTAACGCGGAGTTACCAGCATTCACGCTGATGG
TCATCGCGCTTTCGGAAGTCGTTCAACAGTGGTGGCTGGCAATAGTCGCGTTATTTTTTGTCAGCTTTTTCATGTTCAAG
CGGGCTTACAAACAGTCTCAAAATTTTCGTGACAGCCTCGACCGATTTCTTCTGAAAGTGCCCATTATCGGACCGCTGAT
TTTCAAGTCGTCCGTCGCACGTTATGCACGCACGCTTGCGACAACCTTTGCCGCAGGCGTGCCGTTGGTTGAGGCACTCG
ATTCGGTTGCCGGAGCCACCGGGAACGTCGTATTCAGAAACGCGGTCAATCAGGTCAAACAGGATGTCTCCACTGGCATG
CAACTGAATTTCTCGATGCGGTCAACAGGCGTATTTCCAAGCCTGGCAATACAAATGACAGCAATCGGCGAAGAGTCCGG
TGCGCTTGACGCCATGCTCGATAAAGTCGCTACCTACTATGAAGAGGAAGTCGACAACATGGTCGACAACATGACCAGCC
TGATGGAACCCATGATCATGGCTGTGCTGGGTGTCATCGTCGGCGGCCTTGTCATTGCGATGTACCTCCCCATCTTCAAA
CTTGGAAGCGTCGTCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(276-397)

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

73.333

100

0.733
  pilC Acinetobacter baylyi ADP1

59.069

100

0.595
  pilC Acinetobacter baumannii D1279779

58.416

99.753

0.583
  pilC Legionella pneumophila strain ERS1305867

52.273

97.778

0.511
  pilC Vibrio campbellii strain DS40M4

42.015

100

0.422
  pilG Neisseria gonorrhoeae MS11

41.687

99.506

0.415
  pilG Neisseria meningitidis 44/76-A

41.687

99.506

0.415
  pilC Vibrio cholerae strain A1552

41.481

100

0.415
  pilC Thermus thermophilus HB27

38.06

99.259

0.378