Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   I9H08_RS03750 Genome accession   NZ_CP066263
Coordinates   865110..865982 (-) Length   290 a.a.
NCBI ID   WP_103360221.1    Uniprot ID   -
Organism   Pseudomonas syringae pv. syringae strain Pss9644     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 860110..870982
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I9H08_RS03735 (I9H08_03710) - 863696..864157 (+) 462 WP_058445857.1 hypothetical protein -
  I9H08_RS03740 (I9H08_03715) yacG 864284..864493 (-) 210 WP_002552020.1 DNA gyrase inhibitor YacG -
  I9H08_RS03745 (I9H08_03720) coaE 864490..865113 (-) 624 WP_074909035.1 dephospho-CoA kinase -
  I9H08_RS03750 (I9H08_03725) pilD 865110..865982 (-) 873 WP_103360221.1 A24 family peptidase Machinery gene
  I9H08_RS03755 (I9H08_03730) pilC 865984..867201 (-) 1218 WP_103360220.1 type II secretion system F family protein Machinery gene
  I9H08_RS03760 (I9H08_03735) pilB 867204..868898 (-) 1695 WP_024684410.1 type IV-A pilus assembly ATPase PilB Machinery gene
  I9H08_RS03765 (I9H08_03740) - 869124..869519 (+) 396 WP_103360219.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -

Sequence

Protein


Download         Length: 290 a.a.        Molecular weight: 32009.23 Da        Isoelectric Point: 8.5797

>NTDB_id=518967 I9H08_RS03750 WP_103360221.1 865110..865982(-) (pilD) [Pseudomonas syringae pv. syringae strain Pss9644]
MPLLDLLASSPLAFVTTCCILGLIIGSFLNVVVYRLPIMMERGWKAQSRELLGLPAEPDQPVFNLNRPRSSCPHCAHKIR
PWENLPVISYLLLRGKCSKCKAPISKRYPLVELTCAVLSAYVAWHFDFGWQAAAMLVLSWGLLAMSLIDADHQLLPDSLV
LPLLWLGLIVNAFGLFTSLNDALWGAVAGYLALWSVFWLFKLVTGKEGMGYGDFKLLAMLGAWGGWQILPLTILLSSLVG
AVIGVIMMRVRRVESGTPIPFGPYLAIAGWIALLWGGQITDSYMQFAGFR

Nucleotide


Download         Length: 873 bp        

>NTDB_id=518967 I9H08_RS03750 WP_103360221.1 865110..865982(-) (pilD) [Pseudomonas syringae pv. syringae strain Pss9644]
ATGCCCCTCCTCGACCTCCTGGCCAGCTCCCCCCTGGCCTTCGTCACCACCTGCTGCATCCTCGGCCTGATCATCGGAAG
CTTCCTCAACGTGGTCGTCTACCGCCTCCCGATAATGATGGAGCGCGGCTGGAAAGCCCAATCCCGAGAACTGCTCGGCC
TGCCCGCCGAACCCGATCAGCCCGTCTTCAACCTGAACCGCCCCCGTTCCAGCTGCCCGCATTGCGCACACAAAATCCGC
CCGTGGGAGAACCTGCCGGTGATCAGCTATCTGCTGCTGCGCGGCAAATGCTCTAAGTGCAAAGCGCCGATCAGCAAACG
CTACCCGCTGGTCGAGTTGACCTGCGCAGTGCTTTCAGCCTACGTGGCCTGGCATTTCGACTTTGGCTGGCAGGCGGCGG
CGATGCTGGTGTTGAGCTGGGGCCTGCTGGCGATGAGTCTGATCGATGCTGATCACCAGCTGTTACCTGATTCTCTGGTG
CTGCCACTGCTGTGGCTGGGCCTGATCGTCAACGCCTTCGGGCTGTTCACCTCGCTGAATGATGCGCTGTGGGGTGCAGT
GGCCGGCTATCTGGCGCTGTGGTCGGTGTTCTGGCTGTTCAAGCTGGTGACCGGCAAGGAAGGCATGGGTTATGGCGACT
TCAAGTTGCTGGCCATGCTGGGCGCGTGGGGCGGCTGGCAGATTTTGCCGTTGACCATCCTGTTATCGTCGCTGGTGGGC
GCGGTGATTGGGGTGATCATGATGCGTGTGCGCAGGGTCGAAAGCGGTACGCCGATCCCCTTTGGTCCTTATCTGGCCAT
TGCGGGGTGGATCGCTTTGCTCTGGGGTGGTCAAATAACCGACTCTTACATGCAGTTTGCCGGTTTCAGATGA

Domains

Predicted by InterproScan.

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(20-126)

Type IV leader peptidase family (Peptidase_A24)

(136-245)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

53.357

97.586

0.521
  pilD Vibrio campbellii strain DS40M4

51.943

97.586

0.507
  pilD Acinetobacter baumannii D1279779

52.19

94.483

0.493
  pilD Acinetobacter nosocomialis M2

51.46

94.483

0.486
  pilD Neisseria gonorrhoeae MS11

51.493

92.414

0.476