Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   I9H08_RS03760 Genome accession   NZ_CP066263
Coordinates   867204..868898 (-) Length   564 a.a.
NCBI ID   WP_024684410.1    Uniprot ID   -
Organism   Pseudomonas syringae pv. syringae strain Pss9644     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 862204..873898
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I9H08_RS03735 (I9H08_03710) - 863696..864157 (+) 462 WP_058445857.1 hypothetical protein -
  I9H08_RS03740 (I9H08_03715) yacG 864284..864493 (-) 210 WP_002552020.1 DNA gyrase inhibitor YacG -
  I9H08_RS03745 (I9H08_03720) coaE 864490..865113 (-) 624 WP_074909035.1 dephospho-CoA kinase -
  I9H08_RS03750 (I9H08_03725) pilD 865110..865982 (-) 873 WP_103360221.1 A24 family peptidase Machinery gene
  I9H08_RS03755 (I9H08_03730) pilC 865984..867201 (-) 1218 WP_103360220.1 type II secretion system F family protein Machinery gene
  I9H08_RS03760 (I9H08_03735) pilB 867204..868898 (-) 1695 WP_024684410.1 type IV-A pilus assembly ATPase PilB Machinery gene
  I9H08_RS03765 (I9H08_03740) - 869124..869519 (+) 396 WP_103360219.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  I9H08_RS03770 (I9H08_03745) - 869591..870988 (+) 1398 WP_103360218.1 O-antigen ligase family protein -
  I9H08_RS03775 (I9H08_03750) - 871100..872386 (+) 1287 WP_103360217.1 polysaccharide biosynthesis C-terminal domain-containing protein -
  I9H08_RS03780 (I9H08_03755) - 872434..872979 (-) 546 WP_103360216.1 DUF1523 family protein -

Sequence

Protein


Download         Length: 564 a.a.        Molecular weight: 62498.82 Da        Isoelectric Point: 6.2561

>NTDB_id=518969 I9H08_RS03760 WP_024684410.1 867204..868898(-) (pilB) [Pseudomonas syringae pv. syringae strain Pss9644]
MSDAVLTGLAKQLVLAELLTETAAQQAYQQARRDKVSLVSYLVQNKLVKSLTLAEMASDQFGVPFMDLAGLDKESQPKGL
VSEKLVRQHHALPLWRRGNKLFIGISDPTNHQAVTDIQFSTGLNTEAILVEDDKLTIAIDRFFDTESGLGELADIDLGLD
IEPASDKETSLATQSDADDAPVVRFVNKMLMDAIRLGSSDLHFEPYEKIFRVRLRTDGILHEVAKPPIHLANRIAARLKV
MASLDISERRKPQDGRVKLRVSKNKAIDFRMNTLPTLWGEKIVMRILDPTSAQMGIDALGYEPEQKALYMEALKQPQGMI
LVTGPTGSGKTVSLYTGLNILNTVDINISTAEDPVEINLEGINQVNVNPRQGLDFSQALRAFLRQDPDVIMVGEIRDLET
AEIAIKASQTGHMVLSTLHTNSAAETLTRLHHMGVAAFNIATAINLIIAQRLARKLCSHCKKELDIPRETLIREGFPEEK
IGTFKIYGPVGCEHCNGGYRGRVGIYEVVKKTPELERIIMEEGNSLEISRQMRKDGFNDLRTSGLSKAMQGITSLEEVNR
VTKD

Nucleotide


Download         Length: 1695 bp        

>NTDB_id=518969 I9H08_RS03760 WP_024684410.1 867204..868898(-) (pilB) [Pseudomonas syringae pv. syringae strain Pss9644]
ATGTCTGATGCTGTCCTCACCGGTTTGGCCAAACAATTGGTCCTGGCCGAGCTACTTACTGAAACGGCTGCTCAGCAGGC
TTACCAACAGGCCCGCCGCGACAAGGTTTCACTGGTGAGCTATCTGGTCCAGAACAAACTGGTCAAAAGCCTGACGCTTG
CAGAAATGGCCTCGGACCAGTTTGGCGTGCCCTTCATGGACCTTGCCGGTCTGGACAAGGAAAGCCAGCCCAAGGGGCTG
GTCAGTGAAAAGCTGGTCCGTCAGCATCATGCATTGCCGCTGTGGCGACGTGGTAACAAGCTGTTTATAGGCATCTCGGA
TCCGACCAACCACCAAGCGGTCACGGACATACAGTTCAGCACCGGCCTGAACACCGAAGCGATTCTGGTTGAAGATGACA
AGCTGACCATTGCCATAGACCGTTTTTTTGACACTGAAAGTGGCCTTGGGGAACTGGCGGATATCGACCTTGGCCTGGAC
ATTGAGCCTGCAAGTGACAAGGAAACCTCCCTGGCCACTCAGAGTGATGCCGATGATGCTCCAGTGGTGCGCTTCGTCAA
CAAAATGCTGATGGATGCAATACGCCTAGGGTCATCCGACCTGCACTTTGAACCTTACGAAAAGATCTTTCGCGTGCGCC
TGCGCACCGACGGTATTTTGCATGAAGTGGCCAAACCACCGATTCATCTGGCCAACCGAATTGCTGCGCGGCTGAAAGTT
ATGGCCAGCCTCGATATCTCGGAACGACGAAAACCGCAGGACGGCAGAGTCAAGCTGCGAGTGTCCAAAAACAAAGCGAT
CGACTTCCGTATGAATACGCTGCCAACACTATGGGGCGAGAAGATCGTGATGCGGATTCTCGACCCGACTAGCGCGCAGA
TGGGCATTGATGCACTGGGCTACGAACCGGAGCAAAAGGCTCTTTATATGGAGGCCTTGAAACAGCCCCAAGGCATGATT
CTTGTCACCGGCCCGACTGGGTCAGGCAAAACCGTTTCGCTATACACCGGCCTCAACATTCTCAACACCGTAGACATCAA
CATTTCCACAGCTGAAGACCCGGTAGAGATCAACCTGGAGGGTATCAATCAGGTCAATGTCAATCCGCGACAGGGTCTGG
ACTTTTCCCAGGCATTGCGTGCCTTTCTGCGTCAAGATCCCGACGTGATCATGGTCGGCGAGATCCGGGACCTGGAAACC
GCCGAAATAGCCATCAAAGCCTCGCAAACCGGTCACATGGTGTTATCGACCCTGCACACCAACAGCGCTGCGGAAACCCT
GACTCGCCTGCACCATATGGGCGTCGCAGCGTTCAACATCGCTACAGCAATCAACCTCATCATTGCCCAGCGGCTGGCAC
GCAAGCTATGCAGTCATTGCAAAAAAGAACTCGACATACCTCGTGAAACACTCATACGAGAGGGCTTCCCGGAAGAAAAA
ATAGGTACGTTCAAAATCTACGGCCCTGTTGGCTGCGAGCATTGCAATGGCGGCTATAGAGGCAGGGTCGGGATCTACGA
AGTGGTCAAGAAAACACCTGAACTGGAACGCATCATCATGGAAGAAGGCAATTCGCTGGAGATCTCCAGGCAGATGCGAA
AGGACGGCTTCAATGACTTGAGGACCTCCGGCCTGTCGAAGGCCATGCAAGGCATTACCAGCCTTGAAGAAGTCAACCGC
GTGACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(188-455)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

58.511

100

0.585
  pilB Acinetobacter baylyi ADP1

57.295

99.645

0.571
  pilB Legionella pneumophila strain ERS1305867

55.477

100

0.557
  pilB Vibrio cholerae strain A1552

50.624

99.468

0.504
  pilB Vibrio parahaemolyticus RIMD 2210633

50.181

98.227

0.493
  pilB Vibrio campbellii strain DS40M4

49.113

100

0.491
  pilF Neisseria gonorrhoeae MS11

48.043

99.645

0.479
  pilF Thermus thermophilus HB27

39.399

100

0.395
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.469

93.44

0.369