Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA2   Type   Machinery gene
Locus tag   JC616_RS02460 Genome accession   NZ_CP066126
Coordinates   544909..545331 (+) Length   140 a.a.
NCBI ID   WP_319792917.1    Uniprot ID   -
Organism   Chromobacterium rhizoryzae strain 32279     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 539909..550331
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JC616_RS02440 proB 540451..541569 (-) 1119 WP_107797916.1 glutamate 5-kinase -
  JC616_RS02445 - 541759..543261 (-) 1503 WP_227106564.1 hypothetical protein -
  JC616_RS02450 - 543364..544716 (-) 1353 WP_227106566.1 adenosylmethionine--8-amino-7-oxononanoate transaminase -
  JC616_RS02460 pilA2 544909..545331 (+) 423 WP_319792917.1 pilin Machinery gene
  JC616_RS24490 pilA 545351..545803 (+) 453 WP_256477540.1 pilin Machinery gene
  JC616_RS02475 - 545857..547674 (+) 1818 WP_227106568.1 PglL family O-oligosaccharyltransferase -
  JC616_RS02480 - 547750..549534 (+) 1785 WP_227106570.1 PglL family O-oligosaccharyltransferase -

Sequence

Protein


Download         Length: 140 a.a.        Molecular weight: 14637.83 Da        Isoelectric Point: 8.4893

>NTDB_id=517632 JC616_RS02460 WP_319792917.1 544909..545331(+) (pilA2) [Chromobacterium rhizoryzae strain 32279]
MRKAAGFTLIELMIVVAIIGILAAIAIPAYQDYVIRSKVSEGLTFLDHAKTTVSESYLANNTWPANNASAGMPSSLSGKT
VSDVRISLSNGVSIITATFQASAVPGLTVVFTPSVASGNAVQWLCSGQGQNIQYLPSSCR

Nucleotide


Download         Length: 423 bp        

>NTDB_id=517632 JC616_RS02460 WP_319792917.1 544909..545331(+) (pilA2) [Chromobacterium rhizoryzae strain 32279]
ATGAGGAAGGCGGCTGGTTTTACCTTGATTGAACTAATGATTGTTGTCGCCATTATCGGCATTTTGGCGGCGATTGCGAT
TCCGGCTTATCAGGACTATGTCATTCGTTCAAAAGTTTCTGAAGGGCTGACTTTTTTGGATCATGCAAAAACCACGGTTA
GTGAGTCTTATCTAGCGAATAATACATGGCCGGCTAATAATGCTAGTGCTGGGATGCCAAGCTCTCTGTCTGGCAAGACT
GTTAGTGATGTTCGAATTAGTCTAAGTAATGGGGTGAGTATCATTACTGCTACATTTCAGGCAAGTGCAGTGCCTGGGTT
GACTGTAGTGTTCACCCCAAGTGTGGCAAGCGGTAATGCAGTGCAGTGGTTGTGTTCAGGGCAAGGGCAAAATATTCAAT
ACTTACCGTCATCGTGTCGGTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)

Pilin (bacterial filament) (Pilin)

(36-140)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA2 Legionella pneumophila str. Paris

52.857

100

0.529
  pilA2 Legionella pneumophila strain ERS1305867

52.143

100

0.521
  comP Acinetobacter baylyi ADP1

46.405

100

0.507
  pilE Neisseria gonorrhoeae MS11

40.506

100

0.457
  pilA Ralstonia pseudosolanacearum GMI1000

37.576

100

0.443
  pilE Neisseria gonorrhoeae strain FA1090

38.217

100

0.429
  pilA Pseudomonas aeruginosa PAK

37.5

100

0.407
  pilA/pilA1 Eikenella corrodens VA1

37.671

100

0.393
  pilA Vibrio parahaemolyticus RIMD 2210633

41.6

89.286

0.371