Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGC   Type   Machinery gene
Locus tag   LLNZ_RS12050 Genome accession   NC_017949
Coordinates   2345753..2346130 (-) Length   125 a.a.
NCBI ID   WP_011836046.1    Uniprot ID   A2RNS7
Organism   Lactococcus cremoris subsp. cremoris NZ9000     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
ICE 2339819..2353784 2345753..2346130 within 0


Gene organization within MGE regions


Location: 2339819..2353784
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LLNZ_RS13310 - 2339819..2341214 (-) 1396 Protein_2339 IS3 family transposase -
  LLNZ_RS12010 (LLNZ_12395) - 2341287..2342096 (-) 810 WP_011836038.1 metal ABC transporter permease -
  LLNZ_RS12015 (LLNZ_12400) - 2342089..2342826 (-) 738 WP_011836039.1 metal ABC transporter ATP-binding protein -
  LLNZ_RS12020 (LLNZ_12405) - 2343005..2343847 (-) 843 WP_011836040.1 metal ABC transporter solute-binding protein, Zn/Mn family -
  LLNZ_RS12025 (LLNZ_12410) - 2343844..2344281 (-) 438 WP_011836041.1 zinc-dependent MarR family transcriptional regulator -
  LLNZ_RS12030 (LLNZ_12415) comGG 2344362..2344661 (-) 300 WP_011836042.1 competence type IV pilus minor pilin ComGG Machinery gene
  LLNZ_RS12035 (LLNZ_12420) comGF 2344685..2345110 (-) 426 WP_014735174.1 competence type IV pilus minor pilin ComGF Machinery gene
  LLNZ_RS12040 (LLNZ_12425) comGE 2345094..2345390 (-) 297 WP_014735175.1 competence type IV pilus minor pilin ComGE Machinery gene
  LLNZ_RS12045 (LLNZ_12430) comGD 2345362..2345805 (-) 444 WP_011836045.1 competence type IV pilus minor pilin ComGD Machinery gene
  LLNZ_RS12050 (LLNZ_12435) comGC 2345753..2346130 (-) 378 WP_011836046.1 competence type IV pilus major pilin ComGC Machinery gene
  LLNZ_RS12055 (LLNZ_12440) comGB 2346148..2347173 (-) 1026 WP_042211510.1 competence type IV pilus assembly protein ComGB Machinery gene
  LLNZ_RS12060 (LLNZ_12445) comGA 2347073..2348053 (-) 981 WP_011836048.1 competence type IV pilus ATPase ComGA Machinery gene
  LLNZ_RS12065 (LLNZ_12450) - 2348167..2351949 (-) 3783 Protein_2351 PolC-type DNA polymerase III -
  LLNZ_RS14065 - 2352481..2353074 (-) 594 Protein_2352 PolC-type DNA polymerase III N-terminal domain-containing protein -
  LLNZ_RS12070 (LLNZ_12455) - 2353248..2353784 (-) 537 WP_011836050.1 hypothetical protein -

Sequence

Protein


Download         Length: 125 a.a.        Molecular weight: 14331.79 Da        Isoelectric Point: 8.3991

>NTDB_id=51438 LLNZ_RS12050 WP_011836046.1 2345753..2346130(-) (comGC) [Lactococcus cremoris subsp. cremoris NZ9000]
MKIENITLIMSKALSLVKIHGRKLWQKKQKAFTLIEMLIVLAIISILILLFVPNLIKEKAQVQKTGEAAVVKVVESQAQL
YELDHDNDKPNLSELLSAGMITKKQVTAYDDYYDQNKNEQRNFDD

Nucleotide


Download         Length: 378 bp        

>NTDB_id=51438 LLNZ_RS12050 WP_011836046.1 2345753..2346130(-) (comGC) [Lactococcus cremoris subsp. cremoris NZ9000]
ATGAAAATAGAAAATATAACTTTAATAATGAGCAAAGCTTTGTCATTAGTTAAAATTCACGGAAGAAAGCTTTGGCAAAA
AAAGCAAAAAGCATTTACCTTGATTGAGATGTTAATTGTGTTGGCAATTATCAGTATTTTAATTTTGCTTTTTGTGCCAA
ATTTGATAAAAGAAAAAGCACAAGTTCAAAAAACTGGTGAAGCAGCAGTAGTTAAAGTAGTTGAAAGTCAGGCACAACTT
TATGAGTTGGATCATGATAATGATAAGCCAAACCTATCAGAACTTTTAAGTGCGGGGATGATTACGAAAAAGCAAGTCAC
GGCCTATGATGATTACTATGACCAGAATAAAAATGAACAGCGCAATTTCGATGATTAG

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(27-50)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A2RNS7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGC Lactococcus lactis subsp. cremoris KW2

98.276

92.8

0.912
  comYC Streptococcus gordonii str. Challis substr. CH1

57.576

79.2

0.456
  comGC/cglC Streptococcus mitis SK321

55.882

81.6

0.456
  comGC/cglC Streptococcus pneumoniae R6

53.922

81.6

0.44
  comGC/cglC Streptococcus pneumoniae TIGR4

53.922

81.6

0.44
  comGC/cglC Streptococcus pneumoniae Rx1

53.922

81.6

0.44
  comGC/cglC Streptococcus pneumoniae D39

53.922

81.6

0.44
  comYC Streptococcus mutans UA140

58.065

74.4

0.432
  comYC Streptococcus mutans UA159

58.065

74.4

0.432
  comGC/cglC Streptococcus mitis NCTC 12261

55.67

77.6

0.432
  comYC Streptococcus suis isolate S10

56.818

70.4

0.4

Multiple sequence alignment