Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF   Type   Machinery gene
Locus tag   LLNZ_RS12035 Genome accession   NC_017949
Coordinates   2344685..2345110 (-) Length   141 a.a.
NCBI ID   WP_014735174.1    Uniprot ID   -
Organism   Lactococcus cremoris subsp. cremoris NZ9000     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
ICE 2339819..2353784 2344685..2345110 within 0


Gene organization within MGE regions


Location: 2339819..2353784
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LLNZ_RS13310 - 2339819..2341214 (-) 1396 Protein_2339 IS3 family transposase -
  LLNZ_RS12010 (LLNZ_12395) - 2341287..2342096 (-) 810 WP_011836038.1 metal ABC transporter permease -
  LLNZ_RS12015 (LLNZ_12400) - 2342089..2342826 (-) 738 WP_011836039.1 metal ABC transporter ATP-binding protein -
  LLNZ_RS12020 (LLNZ_12405) - 2343005..2343847 (-) 843 WP_011836040.1 metal ABC transporter solute-binding protein, Zn/Mn family -
  LLNZ_RS12025 (LLNZ_12410) - 2343844..2344281 (-) 438 WP_011836041.1 zinc-dependent MarR family transcriptional regulator -
  LLNZ_RS12030 (LLNZ_12415) comGG 2344362..2344661 (-) 300 WP_011836042.1 competence type IV pilus minor pilin ComGG Machinery gene
  LLNZ_RS12035 (LLNZ_12420) comGF 2344685..2345110 (-) 426 WP_014735174.1 competence type IV pilus minor pilin ComGF Machinery gene
  LLNZ_RS12040 (LLNZ_12425) comGE 2345094..2345390 (-) 297 WP_014735175.1 competence type IV pilus minor pilin ComGE Machinery gene
  LLNZ_RS12045 (LLNZ_12430) comGD 2345362..2345805 (-) 444 WP_011836045.1 competence type IV pilus minor pilin ComGD Machinery gene
  LLNZ_RS12050 (LLNZ_12435) comGC 2345753..2346130 (-) 378 WP_011836046.1 competence type IV pilus major pilin ComGC Machinery gene
  LLNZ_RS12055 (LLNZ_12440) comGB 2346148..2347173 (-) 1026 WP_042211510.1 competence type IV pilus assembly protein ComGB Machinery gene
  LLNZ_RS12060 (LLNZ_12445) comGA 2347073..2348053 (-) 981 WP_011836048.1 competence type IV pilus ATPase ComGA Machinery gene
  LLNZ_RS12065 (LLNZ_12450) - 2348167..2351949 (-) 3783 Protein_2351 PolC-type DNA polymerase III -
  LLNZ_RS14065 - 2352481..2353074 (-) 594 Protein_2352 PolC-type DNA polymerase III N-terminal domain-containing protein -
  LLNZ_RS12070 (LLNZ_12455) - 2353248..2353784 (-) 537 WP_011836050.1 hypothetical protein -

Sequence

Protein


Download         Length: 141 a.a.        Molecular weight: 16210.78 Da        Isoelectric Point: 8.4549

>NTDB_id=51435 LLNZ_RS12035 WP_014735174.1 2344685..2345110(-) (comGF) [Lactococcus cremoris subsp. cremoris NZ9000]
MKLKIRAFTLLECLVALLAISGSVLVISGLTRMIEEQMKISQNDSRKDWQIFCEQMRSELSGAKLDNVNQNFLYVTKDKK
LRFGLVGDDFRKSDDKGQGYQPMLYDLKGAKIQAEENLIKITIDFDNGGERVFIYRFTDTK

Nucleotide


Download         Length: 426 bp        

>NTDB_id=51435 LLNZ_RS12035 WP_014735174.1 2344685..2345110(-) (comGF) [Lactococcus cremoris subsp. cremoris NZ9000]
TTGAAGCTCAAAATTAGGGCATTTACTTTACTAGAATGTCTCGTAGCACTTTTGGCAATTTCTGGTTCAGTGCTAGTCAT
CTCAGGGCTGACTAGAATGATTGAAGAACAGATGAAAATAAGCCAGAATGATAGTAGGAAAGATTGGCAAATATTTTGTG
AACAAATGCGTTCAGAACTCTCCGGAGCAAAATTAGACAATGTGAATCAAAATTTTTTGTATGTGACCAAAGATAAGAAG
TTACGGTTCGGATTAGTAGGGGATGATTTTCGTAAAAGTGATGATAAAGGGCAAGGATACCAACCGATGCTTTATGATTT
AAAAGGAGCAAAAATTCAGGCAGAAGAAAATTTGATAAAAATAACAATTGATTTTGATAATGGAGGTGAGCGAGTATTTA
TTTATCGATTTACAGATACAAAGTAA

Domains

Predicted by InterproScan.

Putative Competence protein ComGF (ComGF)

(38-130)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF Lactococcus lactis subsp. cremoris KW2

100

100

1
  comYF Streptococcus mutans UA140

43.972

100

0.44
  comYF Streptococcus mutans UA159

43.262

100

0.433
  comGF/cglF Streptococcus mitis NCTC 12261

39.286

99.291

0.39
  comGF/cglF Streptococcus mitis SK321

39.286

99.291

0.39
  comGF/cglF Streptococcus pneumoniae Rx1

37.857

99.291

0.376
  comGF/cglF Streptococcus pneumoniae D39

37.857

99.291

0.376
  comGF/cglF Streptococcus pneumoniae R6

37.857

99.291

0.376
  comGF/cglF Streptococcus pneumoniae TIGR4

37.857

99.291

0.376

Multiple sequence alignment