Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   I6G90_RS13950 Genome accession   NZ_CP065745
Coordinates   3075858..3077099 (+) Length   413 a.a.
NCBI ID   WP_139406689.1    Uniprot ID   A0A7T2ULJ4
Organism   Aeromonas allosaccharophila strain FDAARGOS_933     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3070858..3082099
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I6G90_RS13925 (I6G90_13920) ampD 3071257..3071832 (-) 576 WP_197927983.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  I6G90_RS13930 (I6G90_13925) - 3071960..3072430 (+) 471 WP_040068932.1 TIGR02281 family clan AA aspartic protease -
  I6G90_RS13935 (I6G90_13930) nadC 3072434..3073297 (+) 864 WP_197927985.1 carboxylating nicotinate-nucleotide diphosphorylase -
  I6G90_RS13940 (I6G90_13935) - 3073613..3073993 (+) 381 WP_197927987.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  I6G90_RS13945 (I6G90_13940) pilB 3073997..3075703 (+) 1707 WP_197927989.1 PilB family type IVa pilus assembly ATPase TapB Machinery gene
  I6G90_RS13950 (I6G90_13945) pilC 3075858..3077099 (+) 1242 WP_139406689.1 type II secretion system F family protein Machinery gene
  I6G90_RS13955 (I6G90_13950) pilD 3077161..3078033 (+) 873 WP_197927991.1 A24 family peptidase Machinery gene
  I6G90_RS13960 (I6G90_13955) coaE 3078047..3078661 (+) 615 WP_197927993.1 dephospho-CoA kinase -
  I6G90_RS13965 (I6G90_13960) zapD 3078699..3079421 (+) 723 WP_042058692.1 cell division protein ZapD -
  I6G90_RS13970 (I6G90_13965) yacG 3079434..3079628 (+) 195 WP_033138127.1 DNA gyrase inhibitor YacG -
  I6G90_RS13975 (I6G90_13970) mutT 3079706..3080110 (-) 405 WP_197927995.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 413 a.a.        Molecular weight: 45454.54 Da        Isoelectric Point: 9.8298

>NTDB_id=513868 I6G90_RS13950 WP_139406689.1 3075858..3077099(+) (pilC) [Aeromonas allosaccharophila strain FDAARGOS_933]
MATLTKKNNAPKKVFAFRWHGVNRKGQKVSGELQADSINTVKAELRKQGVNVTKVAKKSQGLFSKGGAKIKPMDIAIVSR
QITTMLSAGVPLVQSLQIIARSHEKASMRELMGQIAADVETGTPMSEALRRHPRYFDDLYCDLVEAGEQSGALETIYDRI
ATYREKSEALKSKIKKAMFYPTMVILVAIVVTSILLLFVIPQFEDIFKSFGAELPVFTQFVIGISRFMQNWWYVFFGGSA
LGIFLYVRAWRASQKVRDNTDKFVLTIPVVGMILHKAAMARFARTLSTTFSAGIPLVDALISAAGASGNYVYRTAVMAIR
NEVVAGMQINVAMRTVDLFPDMVIQMVMIGEESGAIDDMLSKVATIFEQEVDDLVDGLTSLLEPLIMVVLGVLVGGMVIA
MYLPIFKLGDVVG

Nucleotide


Download         Length: 1242 bp        

>NTDB_id=513868 I6G90_RS13950 WP_139406689.1 3075858..3077099(+) (pilC) [Aeromonas allosaccharophila strain FDAARGOS_933]
ATGGCAACCCTAACCAAGAAGAACAATGCCCCCAAGAAAGTCTTCGCCTTCCGCTGGCACGGGGTAAACCGCAAGGGGCA
GAAGGTCTCCGGCGAGCTGCAGGCCGACAGCATCAACACCGTCAAGGCCGAACTGCGCAAGCAGGGGGTCAACGTCACCA
AGGTGGCCAAGAAATCCCAGGGGCTCTTTTCCAAGGGCGGAGCCAAGATCAAACCGATGGATATCGCCATCGTCTCCCGC
CAGATCACCACCATGCTCTCCGCCGGTGTACCACTGGTGCAGAGCCTGCAGATCATCGCCCGCAGCCACGAGAAAGCCTC
GATGCGCGAGCTGATGGGGCAGATTGCCGCCGATGTAGAGACCGGTACCCCCATGTCGGAGGCGCTGCGCCGTCACCCCC
GCTACTTTGACGATCTCTACTGCGATCTGGTGGAAGCCGGTGAGCAATCCGGTGCGCTGGAGACCATCTACGACCGTATC
GCCACTTACCGGGAGAAATCGGAAGCACTCAAGTCGAAGATCAAGAAGGCGATGTTCTACCCCACCATGGTCATTCTGGT
CGCCATTGTGGTGACCTCCATCCTGTTGCTGTTCGTCATTCCGCAGTTCGAGGATATCTTCAAAAGCTTCGGTGCCGAGC
TGCCAGTGTTCACCCAGTTTGTCATCGGTATCTCACGCTTTATGCAGAACTGGTGGTATGTCTTCTTTGGTGGCTCAGCC
CTTGGCATCTTCCTCTATGTGCGAGCCTGGCGCGCCTCCCAGAAGGTGAGAGACAACACCGACAAGTTTGTACTCACCAT
TCCGGTGGTCGGGATGATCCTGCACAAGGCGGCGATGGCTCGCTTTGCCCGCACCCTGTCGACCACCTTCTCCGCCGGTA
TTCCACTGGTGGATGCGCTGATTTCGGCGGCCGGAGCCTCCGGCAACTATGTCTATCGCACCGCGGTCATGGCGATCCGC
AACGAGGTAGTAGCCGGTATGCAGATCAACGTGGCGATGCGTACCGTCGATCTCTTCCCCGACATGGTGATCCAGATGGT
GATGATCGGTGAGGAGTCCGGTGCCATCGATGATATGCTCTCCAAGGTCGCCACCATCTTCGAGCAGGAGGTGGATGATC
TGGTCGATGGTCTCACCAGCCTGCTGGAACCCCTTATCATGGTGGTGCTCGGGGTGCTGGTCGGCGGCATGGTTATCGCC
ATGTACCTGCCCATCTTCAAGCTGGGCGACGTGGTAGGCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(282-404)

Type II secretion system (T2SS), protein F (T2SSF)

(79-201)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7T2ULJ4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

57.828

95.884

0.554
  pilC Acinetobacter baumannii D1279779

54

96.852

0.523
  pilC Acinetobacter baylyi ADP1

53.133

96.61

0.513
  pilC Legionella pneumophila strain ERS1305867

50.985

98.305

0.501
  pilC Vibrio cholerae strain A1552

47.132

97.094

0.458
  pilC Vibrio campbellii strain DS40M4

46.482

96.368

0.448
  pilG Neisseria gonorrhoeae MS11

40.247

98.063

0.395
  pilG Neisseria meningitidis 44/76-A

40

98.063

0.392
  pilC Thermus thermophilus HB27

38.25

96.852

0.37