Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   I6G90_RS13945 Genome accession   NZ_CP065745
Coordinates   3073997..3075703 (+) Length   568 a.a.
NCBI ID   WP_197927989.1    Uniprot ID   A0A7T2ULU2
Organism   Aeromonas allosaccharophila strain FDAARGOS_933     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3068997..3080703
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I6G90_RS13920 (I6G90_13915) pdhR 3070214..3070978 (-) 765 WP_058053405.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  I6G90_RS13925 (I6G90_13920) ampD 3071257..3071832 (-) 576 WP_197927983.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  I6G90_RS13930 (I6G90_13925) - 3071960..3072430 (+) 471 WP_040068932.1 TIGR02281 family clan AA aspartic protease -
  I6G90_RS13935 (I6G90_13930) nadC 3072434..3073297 (+) 864 WP_197927985.1 carboxylating nicotinate-nucleotide diphosphorylase -
  I6G90_RS13940 (I6G90_13935) - 3073613..3073993 (+) 381 WP_197927987.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  I6G90_RS13945 (I6G90_13940) pilB 3073997..3075703 (+) 1707 WP_197927989.1 PilB family type IVa pilus assembly ATPase TapB Machinery gene
  I6G90_RS13950 (I6G90_13945) pilC 3075858..3077099 (+) 1242 WP_139406689.1 type II secretion system F family protein Machinery gene
  I6G90_RS13955 (I6G90_13950) pilD 3077161..3078033 (+) 873 WP_197927991.1 A24 family peptidase Machinery gene
  I6G90_RS13960 (I6G90_13955) coaE 3078047..3078661 (+) 615 WP_197927993.1 dephospho-CoA kinase -
  I6G90_RS13965 (I6G90_13960) zapD 3078699..3079421 (+) 723 WP_042058692.1 cell division protein ZapD -
  I6G90_RS13970 (I6G90_13965) yacG 3079434..3079628 (+) 195 WP_033138127.1 DNA gyrase inhibitor YacG -
  I6G90_RS13975 (I6G90_13970) mutT 3079706..3080110 (-) 405 WP_197927995.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62531.92 Da        Isoelectric Point: 5.4321

>NTDB_id=513867 I6G90_RS13945 WP_197927989.1 3073997..3075703(+) (pilB) [Aeromonas allosaccharophila strain FDAARGOS_933]
MTSSPNSGLALSLAASSLISEEDSQRYLSQAKAQRKPFVTFLIENNILDSKALADFCELEYGVPLLDLAAFDLAEIPQKF
LNQKLIDKHHVLPIYTQGHTLYIAMSDPTNVSALEDFGFSFGLHTEALLVEENKLTAAIAKLLENENDALGMEDIDESEI
SDLEVSDENSRLDESVNTADDDAPIVKYINKIMMDAIKRGASDLHFEPYEFKYRVRFRIDGILHEIATPPVNLANRFSAR
LKVMARLDIAERRLPQDGRIKLKLSRNKSMDMRVNTLPTMWGEKIVIRLLDSSAARLNIEQLGFDDRQKAQYLRALSKPQ
GMILVTGPTGSGKTVSLYTGLNILNTTEVNISTAEDPVEINLPGVNQVQVNPKAGLTFASALRSFLRQDPDVVMVGEIRD
LETAEIAIKAAQTGHLVLSTLHTNSAAETLTRMMNMGVPAFNIASSVTLIMAQRLARKLCDHCKAPEVVPEAELLELGFT
PQQLAAGLRLFKPVGCKECSGGYKGRVGIYEIMLMSENIAKLIMQGANSLQIAAIAQKEGMRTLRTSGLEKARLGVTSLA
EVNRVTTN

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=513867 I6G90_RS13945 WP_197927989.1 3073997..3075703(+) (pilB) [Aeromonas allosaccharophila strain FDAARGOS_933]
ATGACCTCTAGCCCTAATAGCGGCTTGGCGTTAAGCCTGGCCGCCTCTTCCCTCATTAGTGAAGAGGACTCCCAGCGCTA
TCTGAGCCAGGCCAAGGCCCAGCGCAAACCCTTTGTGACCTTTCTGATCGAGAACAACATCCTCGACAGCAAGGCATTGG
CCGATTTTTGCGAGCTGGAGTATGGGGTACCTCTGCTGGATCTTGCCGCCTTCGATTTGGCCGAGATCCCGCAGAAGTTC
CTCAATCAAAAGCTGATCGACAAGCACCACGTCCTGCCCATCTACACCCAGGGCCATACCCTCTATATCGCCATGTCGGA
TCCGACCAATGTGTCGGCACTGGAGGATTTCGGCTTCAGCTTCGGGCTCCATACCGAGGCGCTGCTGGTAGAGGAAAACA
AGCTCACCGCCGCCATAGCCAAGCTGCTGGAGAACGAGAACGACGCCCTCGGCATGGAAGATATCGACGAGTCCGAGATT
TCGGATCTCGAGGTCTCCGACGAGAACAGTCGGCTCGACGAGAGCGTCAATACTGCCGATGACGATGCCCCCATCGTCAA
GTACATCAACAAGATCATGATGGATGCCATCAAGCGCGGCGCCTCCGACCTGCACTTCGAACCCTACGAGTTTAAGTATC
GGGTACGCTTTCGCATCGACGGCATCTTGCACGAGATCGCCACCCCGCCGGTCAATCTGGCCAACCGCTTCTCCGCCCGC
CTCAAGGTAATGGCGCGGCTCGATATCGCCGAGCGGCGGCTGCCGCAGGATGGCCGCATCAAGCTGAAGCTGTCGCGCAA
CAAGTCGATGGATATGCGGGTCAACACCCTGCCCACCATGTGGGGGGAGAAGATCGTGATCCGTCTGCTCGACTCCTCCG
CTGCGCGGCTCAATATCGAACAACTCGGCTTTGACGACCGGCAGAAGGCGCAATATCTGCGTGCCCTCTCCAAGCCGCAG
GGGATGATCCTGGTGACCGGCCCCACCGGCTCGGGTAAAACCGTCTCGCTCTATACCGGTCTCAATATTCTCAACACCAC
TGAGGTGAACATCTCCACCGCCGAAGATCCGGTAGAGATCAACTTGCCCGGCGTCAACCAGGTGCAGGTCAACCCCAAGG
CGGGGCTCACCTTTGCCAGCGCCCTGCGCTCCTTCTTGCGGCAAGACCCGGACGTGGTGATGGTGGGGGAGATCCGCGAT
CTCGAAACCGCCGAAATCGCCATCAAGGCAGCCCAAACCGGCCACCTGGTGCTCTCCACCCTGCACACCAACTCCGCCGC
CGAGACCCTGACCCGGATGATGAACATGGGGGTGCCCGCCTTCAACATCGCCTCCTCGGTGACCCTGATCATGGCCCAGC
GGCTGGCCCGTAAGCTGTGCGACCACTGCAAGGCGCCGGAGGTGGTGCCAGAAGCCGAACTGCTGGAGCTGGGCTTTACC
CCACAGCAACTGGCGGCCGGATTACGACTGTTCAAACCGGTCGGTTGCAAGGAGTGTTCCGGCGGCTACAAGGGGCGGGT
CGGCATCTACGAAATCATGTTGATGTCGGAGAACATCGCCAAATTGATCATGCAGGGGGCTAACTCGCTGCAAATCGCCG
CGATTGCCCAGAAAGAGGGGATGCGGACTCTGCGAACCTCGGGGCTTGAGAAGGCCCGACTCGGGGTCACCAGTCTGGCC
GAGGTCAACCGGGTTACCACCAACTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-146)

Type II/IV secretion system protein (T2SSE)

(191-458)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7T2ULU2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

55.789

100

0.56
  pilB Acinetobacter baumannii D1279779

55.81

100

0.558
  pilB Legionella pneumophila strain ERS1305867

56.273

95.423

0.537
  pilB Vibrio cholerae strain A1552

54.399

98.063

0.533
  pilB Vibrio campbellii strain DS40M4

50.523

100

0.511
  pilB Vibrio parahaemolyticus RIMD 2210633

51.068

98.944

0.505
  pilF Neisseria gonorrhoeae MS11

47.163

99.296

0.468
  pilF Thermus thermophilus HB27

40.53

92.958

0.377
  pilB/pilB1 Synechocystis sp. PCC 6803

38.156

97.359

0.371
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.355

85.211

0.361