Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   I6G86_RS01155 Genome accession   NZ_CP065637
Coordinates   171990..172934 (-) Length   314 a.a.
NCBI ID   WP_042218031.1    Uniprot ID   -
Organism   Lactococcus garvieae strain FDAARGOS_929     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 166990..177934
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I6G86_RS01120 (I6G86_01120) - 167599..168468 (+) 870 WP_042218022.1 RluA family pseudouridine synthase -
  I6G86_RS01125 (I6G86_01125) - 168554..169399 (-) 846 WP_042218024.1 metal ABC transporter solute-binding protein, Zn/Mn family -
  I6G86_RS10755 (I6G86_01130) comYF 169632..170072 (-) 441 WP_042218182.1 competence type IV pilus minor pilin ComGF Machinery gene
  I6G86_RS10760 (I6G86_01135) comGE 170130..170456 (-) 327 Protein_184 competence type IV pilus minor pilin ComGE -
  I6G86_RS01140 (I6G86_01140) comGD 170359..170733 (-) 375 WP_042218026.1 competence type IV pilus minor pilin ComGD -
  I6G86_RS01145 (I6G86_01145) comYC 170708..171013 (-) 306 WP_016171026.1 competence type IV pilus major pilin ComGC Machinery gene
  I6G86_RS01150 (I6G86_01150) comGB 171032..172051 (-) 1020 WP_255204098.1 competence type IV pilus assembly protein ComGB Machinery gene
  I6G86_RS01155 (I6G86_01155) comGA 171990..172934 (-) 945 WP_042218031.1 competence type IV pilus ATPase ComGA Machinery gene
  I6G86_RS01160 (I6G86_01160) rpsU 173131..173307 (-) 177 WP_003134539.1 30S ribosomal protein S21 -
  I6G86_RS01165 (I6G86_01165) guaB 173430..174911 (-) 1482 WP_003133714.1 IMP dehydrogenase -
  I6G86_RS01170 (I6G86_01170) - 175080..176534 (+) 1455 WP_042218033.1 GH25 family lysozyme -

Sequence

Protein


Download         Length: 314 a.a.        Molecular weight: 35889.17 Da        Isoelectric Point: 6.9763

>NTDB_id=512388 I6G86_RS01155 WP_042218031.1 171990..172934(-) (comGA) [Lactococcus garvieae strain FDAARGOS_929]
MIQVLAKKLLQKAVDQMVHDLYFVALEGKYSLYFRTATERRFERELDFEQGQALIAHMKFLSGMNLGESRRVQLGACTYV
LDKGEQRLRLSTVGDFHGQESLVIRLLHHQQSQLHFWNSEIFKSFIGGRGLYLFSGPVGSGKTSLMYKFAREHFKNQQVI
CIEDPVELVETEFLQLQVNKVIGNDYDALIKLSLRHRPDLLIVGEIRDSQTAKAVLRASLTGYTVFSTVHARSISGVIAR
LKELGLTDWELQSSLQRVIYQRLIAGKGLLVYEKEKFEEWQPDEWNGQIDQLVADGFISAVTAAREKIEFSEAD

Nucleotide


Download         Length: 945 bp        

>NTDB_id=512388 I6G86_RS01155 WP_042218031.1 171990..172934(-) (comGA) [Lactococcus garvieae strain FDAARGOS_929]
ATGATACAGGTTTTGGCAAAAAAACTTTTACAAAAAGCAGTAGATCAGATGGTGCATGATCTTTATTTCGTAGCATTAGA
AGGAAAGTATTCTCTCTATTTTCGTACAGCAACAGAAAGGAGGTTTGAAAGAGAACTCGATTTTGAACAAGGGCAAGCAC
TGATTGCTCATATGAAATTTTTATCTGGTATGAATCTAGGAGAAAGTAGGCGGGTACAGTTGGGGGCTTGCACTTATGTT
TTAGATAAAGGTGAGCAGCGTTTACGTTTATCTACAGTCGGTGATTTTCATGGTCAAGAAAGCTTGGTTATCCGACTTCT
GCATCATCAACAAAGCCAACTTCATTTTTGGAATTCAGAGATTTTTAAATCTTTTATTGGTGGGAGAGGGCTTTACCTGT
TTTCCGGTCCCGTAGGCTCAGGTAAGACCAGCCTCATGTACAAGTTTGCAAGGGAGCATTTTAAAAATCAACAAGTTATT
TGTATAGAAGATCCCGTGGAGTTAGTGGAAACTGAGTTTTTACAACTTCAAGTCAATAAAGTAATAGGGAATGATTACGA
TGCTTTGATTAAGCTCTCTCTTCGCCACAGGCCTGATTTACTCATTGTTGGTGAAATTAGGGACAGTCAGACGGCAAAAG
CTGTTCTCCGCGCAAGTTTGACGGGATATACAGTGTTTTCTACTGTTCATGCGCGCTCAATATCGGGTGTAATTGCACGG
CTGAAGGAACTGGGGTTAACTGATTGGGAGCTACAATCCAGCCTTCAGCGGGTGATTTATCAGCGCTTAATTGCAGGAAA
GGGGTTGCTTGTTTATGAAAAAGAAAAGTTTGAAGAATGGCAACCAGATGAATGGAACGGCCAGATTGATCAGTTGGTTG
CAGATGGATTTATCTCAGCTGTTACAGCTGCGCGCGAAAAAATTGAGTTTAGCGAAGCAGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(8-264)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Lactococcus lactis subsp. cremoris KW2

59.873

100

0.599
  comYA Streptococcus gordonii str. Challis substr. CH1

53.968

100

0.541
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

53.674

99.682

0.535
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

53.355

99.682

0.532
  comGA/cglA/cilD Streptococcus pneumoniae D39

53.355

99.682

0.532
  comGA/cglA/cilD Streptococcus pneumoniae R6

53.355

99.682

0.532
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

53.355

99.682

0.532
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

52.396

99.682

0.522
  comYA Streptococcus mutans UA159

51.923

99.363

0.516
  comYA Streptococcus mutans UA140

51.923

99.363

0.516