Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB   Type   Machinery gene
Locus tag   I6G86_RS01150 Genome accession   NZ_CP065637
Coordinates   171032..172051 (-) Length   339 a.a.
NCBI ID   WP_255204098.1    Uniprot ID   -
Organism   Lactococcus garvieae strain FDAARGOS_929     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 166032..177051
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I6G86_RS01120 (I6G86_01120) - 167599..168468 (+) 870 WP_042218022.1 RluA family pseudouridine synthase -
  I6G86_RS01125 (I6G86_01125) - 168554..169399 (-) 846 WP_042218024.1 metal ABC transporter solute-binding protein, Zn/Mn family -
  I6G86_RS10755 (I6G86_01130) comYF 169632..170072 (-) 441 WP_042218182.1 competence type IV pilus minor pilin ComGF Machinery gene
  I6G86_RS10760 (I6G86_01135) comGE 170130..170456 (-) 327 Protein_184 competence type IV pilus minor pilin ComGE -
  I6G86_RS01140 (I6G86_01140) comGD 170359..170733 (-) 375 WP_042218026.1 competence type IV pilus minor pilin ComGD -
  I6G86_RS01145 (I6G86_01145) comYC 170708..171013 (-) 306 WP_016171026.1 competence type IV pilus major pilin ComGC Machinery gene
  I6G86_RS01150 (I6G86_01150) comGB 171032..172051 (-) 1020 WP_255204098.1 competence type IV pilus assembly protein ComGB Machinery gene
  I6G86_RS01155 (I6G86_01155) comGA 171990..172934 (-) 945 WP_042218031.1 competence type IV pilus ATPase ComGA Machinery gene
  I6G86_RS01160 (I6G86_01160) rpsU 173131..173307 (-) 177 WP_003134539.1 30S ribosomal protein S21 -
  I6G86_RS01165 (I6G86_01165) guaB 173430..174911 (-) 1482 WP_003133714.1 IMP dehydrogenase -
  I6G86_RS01170 (I6G86_01170) - 175080..176534 (+) 1455 WP_042218033.1 GH25 family lysozyme -

Sequence

Protein


Download         Length: 339 a.a.        Molecular weight: 39361.05 Da        Isoelectric Point: 9.7389

>NTDB_id=512387 I6G86_RS01150 WP_255204098.1 171032..172051(-) (comGB) [Lactococcus garvieae strain FDAARGOS_929]
MDLSQLLQLRAKKLSLAKQIKLIQLMNNLFKSGFHLSEIIDFLERSGLTEAYFISKMREGLLNGQSFSGILAKLKFNEDI
VTQVSLAESHGNVEYTLGLIEEKLRRVLNIRKKLIQVATYPLVLLAFLIFIMLGLKNYLLPQLDENRGLATYVIQNLPTL
FLGCLFSLLVFFYLGRYYWKKKTALEAMRLMVKIPFVGRFVQLYLTAYFSREWGNLIAQAVDLRQICFIMQEQKSRIFKE
FGFELLQILDRGQKFEDALRLYPIFTKELALIVEYGELKSKLGKELMVFSEESWSLFFERVERAMQLIQPIVFLLVALLI
ILIYAAMLLPIYGNMGNLI

Nucleotide


Download         Length: 1020 bp        

>NTDB_id=512387 I6G86_RS01150 WP_255204098.1 171032..172051(-) (comGB) [Lactococcus garvieae strain FDAARGOS_929]
ATGGATTTATCTCAGCTGTTACAGCTGCGCGCGAAAAAATTGAGTTTAGCGAAGCAGATTAAGCTTATTCAACTTATGAA
TAATCTTTTCAAAAGTGGCTTTCACCTTTCTGAAATTATTGATTTTCTTGAAAGGTCGGGTCTCACGGAAGCATACTTTA
TTTCCAAAATGCGTGAAGGTTTACTCAACGGGCAAAGTTTCTCTGGTATTTTAGCGAAGCTCAAATTTAATGAGGATATT
GTGACTCAAGTCTCTTTGGCCGAGTCTCACGGGAATGTTGAGTATACTTTAGGGTTGATTGAAGAAAAACTGAGACGCGT
TTTGAACATTCGAAAAAAGCTCATCCAAGTTGCCACATACCCGCTCGTTCTTCTGGCATTTCTAATCTTTATCATGCTAG
GGTTGAAAAATTATTTACTGCCTCAACTGGACGAAAACAGAGGATTAGCAACTTATGTTATACAGAATCTTCCGACCCTC
TTTTTAGGGTGCCTCTTCAGTTTGCTTGTTTTCTTTTATCTGGGCAGATACTATTGGAAGAAAAAAACTGCTTTAGAAGC
GATGAGGCTAATGGTCAAAATACCTTTTGTTGGCCGTTTTGTCCAGCTTTATTTGACAGCTTATTTTTCAAGAGAATGGG
GAAACTTAATTGCCCAAGCAGTTGATTTACGTCAGATTTGCTTTATTATGCAAGAACAAAAAAGTCGAATCTTCAAAGAA
TTTGGTTTTGAGCTCCTTCAAATATTAGATAGGGGTCAGAAGTTTGAAGATGCCCTTCGACTTTACCCCATATTTACCAA
GGAGTTGGCATTAATTGTGGAATATGGTGAACTAAAGTCTAAGCTGGGGAAAGAGTTAATGGTTTTTTCTGAAGAAAGCT
GGTCGCTCTTTTTTGAAAGAGTTGAAAGAGCAATGCAACTTATCCAGCCTATCGTTTTCTTGCTCGTGGCGCTGTTGATT
ATTTTAATTTATGCTGCAATGTTACTTCCTATATATGGCAATATGGGAAACTTGATTTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(209-330)

Type II secretion system (T2SS), protein F (T2SSF)

(23-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB Lactococcus lactis subsp. cremoris KW2

53.55

99.705

0.534
  comGB/cglB Streptococcus mitis NCTC 12261

52.836

98.82

0.522
  comGB/cglB Streptococcus pneumoniae D39

52.537

98.82

0.519
  comGB/cglB Streptococcus pneumoniae R6

52.537

98.82

0.519
  comGB/cglB Streptococcus pneumoniae TIGR4

52.537

98.82

0.519
  comGB/cglB Streptococcus mitis SK321

52.537

98.82

0.519
  comGB/cglB Streptococcus pneumoniae Rx1

52.537

98.82

0.519
  comYB Streptococcus gordonii str. Challis substr. CH1

52.083

99.115

0.516
  comYB Streptococcus mutans UA140

51.266

93.215

0.478
  comYB Streptococcus mutans UA159

50.949

93.215

0.475