Detailed information    

insolico Bioinformatically predicted

Overview

Name   comYA   Type   Machinery gene
Locus tag   ST4021_RS08885 Genome accession   NZ_CP065493
Coordinates   1718532..1719473 (-) Length   313 a.a.
NCBI ID   WP_014622005.1    Uniprot ID   A0A2X3UX10
Organism   Streptococcus thermophilus strain 4021     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1713532..1724473
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ST4021_RS08845 (ST4021_08870) - 1713608..1714801 (-) 1194 WP_014608740.1 acetate kinase -
  ST4021_RS08850 (ST4021_08875) comYH 1714857..1715813 (-) 957 WP_011681684.1 class I SAM-dependent methyltransferase Machinery gene
  ST4021_RS08855 (ST4021_08880) comGG 1715858..1716175 (-) 318 WP_011681685.1 competence type IV pilus minor pilin ComGG -
  ST4021_RS08860 (ST4021_08885) comYF 1716153..1716590 (-) 438 WP_011681686.1 competence type IV pilus minor pilin ComGF Machinery gene
  ST4021_RS08865 (ST4021_08890) comGE 1716574..1716867 (-) 294 WP_011226625.1 competence type IV pilus minor pilin ComGE -
  ST4021_RS08870 (ST4021_08895) comYD 1716839..1717267 (-) 429 WP_011226626.1 competence type IV pilus minor pilin ComGD Machinery gene
  ST4021_RS08875 (ST4021_08900) comYC 1717227..1717553 (-) 327 WP_002946126.1 competence type IV pilus major pilin ComGC Machinery gene
  ST4021_RS08880 (ST4021_08905) comYB 1717550..1718650 (-) 1101 WP_120764773.1 competence type IV pilus assembly protein ComGB Machinery gene
  ST4021_RS08885 (ST4021_08910) comYA 1718532..1719473 (-) 942 WP_014622005.1 competence type IV pilus ATPase ComGA Machinery gene
  ST4021_RS08890 (ST4021_08915) - 1719554..1719916 (-) 363 WP_023909982.1 DUF1033 family protein -
  ST4021_RS08895 (ST4021_08920) rpoC 1720075..1723713 (-) 3639 WP_011226631.1 DNA-directed RNA polymerase subunit beta' -

Sequence

Protein


Download         Length: 313 a.a.        Molecular weight: 35421.62 Da        Isoelectric Point: 6.3927

>NTDB_id=510988 ST4021_RS08885 WP_014622005.1 1718532..1719473(-) (comYA) [Streptococcus thermophilus strain 4021]
MVTEFAKEMIKNADSCGAQDIYVIPRQDNYELYMRVGQERRLIDVYRPDFMASLIGHFKFVAGMMVGEKRRSQLGACDYD
CGDGHLVSLRLSTVGDYRGLESLVIRILHSERRELVYWNQGIQPIKDALDYRGLYLFAGPVGSGKTTLMHELVQERFKGQ
QVISIEDPVEIKQDNVLQLQVNQVIDMTYDSLIKLSLRHRPDVLIIGEIRDKETARAVIRASLTGVTVLSTIHAKSIAGV
YERLLDLGVDKSELDNALQGIAYMRLIKGGGVIDFARENFQNHSSTNWNQQLEGLVKQGCLTERDIQGEKIKD

Nucleotide


Download         Length: 942 bp        

>NTDB_id=510988 ST4021_RS08885 WP_014622005.1 1718532..1719473(-) (comYA) [Streptococcus thermophilus strain 4021]
ATGGTAACAGAATTTGCTAAGGAAATGATCAAAAATGCTGATAGTTGTGGGGCTCAAGACATCTATGTCATTCCACGTCA
GGATAATTATGAGCTCTATATGCGAGTCGGGCAAGAGAGGAGATTGATTGATGTATATCGGCCTGATTTCATGGCTAGTC
TTATTGGTCACTTTAAATTTGTGGCGGGAATGATGGTGGGTGAGAAGCGTAGGAGTCAACTGGGTGCATGTGACTATGAT
TGTGGTGACGGTCACTTGGTTTCTCTGCGTTTATCAACTGTTGGAGACTATCGAGGTTTGGAGAGCTTGGTTATCCGTAT
CCTACATTCCGAACGTCGAGAATTAGTGTATTGGAATCAAGGAATCCAGCCTATTAAGGATGCCTTGGATTATAGAGGAC
TGTATCTCTTTGCGGGTCCCGTAGGTTCTGGGAAGACCACACTTATGCATGAGTTGGTTCAGGAGCGTTTTAAGGGGCAG
CAGGTAATTTCGATTGAGGATCCTGTTGAAATCAAACAGGATAATGTCTTGCAACTCCAGGTTAATCAGGTGATTGATAT
GACCTATGATAGTTTGATTAAGCTATCTCTACGTCACCGTCCAGATGTTTTGATTATTGGGGAGATTCGGGATAAGGAGA
CTGCCCGGGCAGTTATTAGAGCTAGTCTAACAGGTGTAACTGTTCTTTCAACTATTCATGCTAAGAGTATTGCTGGCGTT
TACGAACGTCTCTTGGACCTTGGCGTAGATAAGTCTGAGTTGGATAATGCTCTACAAGGCATTGCCTACATGCGTTTGAT
CAAGGGGGGAGGTGTGATTGATTTTGCCAGAGAAAATTTCCAAAACCATTCGTCGACCAACTGGAATCAGCAGTTGGAAG
GTTTGGTTAAACAAGGATGTCTCACTGAGAGGGATATCCAAGGGGAAAAAATTAAAGATTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-267)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2X3UX10

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comYA Streptococcus mutans UA159

64.537

100

0.645
  comYA Streptococcus mutans UA140

64.537

100

0.645
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

64.952

99.361

0.645
  comGA/cglA/cilD Streptococcus pneumoniae D39

63.666

99.361

0.633
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

63.666

99.361

0.633
  comGA/cglA/cilD Streptococcus pneumoniae R6

63.666

99.361

0.633
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

63.666

99.361

0.633
  comYA Streptococcus gordonii str. Challis substr. CH1

62.939

100

0.629
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

61.859

99.681

0.617
  comGA Lactococcus lactis subsp. cremoris KW2

51.768

99.361

0.514
  comGA Latilactobacillus sakei subsp. sakei 23K

38.816

97.125

0.377