Detailed information NTG0051058

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: Q7A_RS07395
• Genome accession: NC_017857
• Coordinates: 1526653..1527756 (+)
• Length: 367 a.a.
• NCBI ID: WP_014706716.1
• Uniprot ID: I1XIX4
• Organism: Methylophaga nitratireducenticrescens
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1521653..1532756

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
Q7A_RS07385 (Q7A_1515)	nagZ	1521962..1523011 (+)	1050	WP_014706714.1	beta-N-acetylhexosaminidase	-
Q7A_RS07390 (Q7A_1516)	dnaE	1523087..1526572 (+)	3486	WP_014706715.1	DNA polymerase III subunit alpha	-
Q7A_RS07395 (Q7A_1517)	pilU	1526653..1527756 (+)	1104	WP_014706716.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
Q7A_RS07400 (Q7A_1518)	-	1528014..1528970 (+)	957	WP_014706717.1	acetyl-CoA carboxylase carboxyltransferase subunit alpha	-
Q7A_RS07405 (Q7A_1519)	tilS	1528991..1530319 (+)	1329	WP_048480984.1	tRNA lysidine(34) synthetase TilS	-
Q7A_RS07410 (Q7A_1520)	-	1530425..1532062 (+)	1638	WP_014706719.1	CTP synthase	-

Sequence

Protein

• Length: 367 a.a.
• Molecular weight: 40998.09 Da
• Isoelectric Point: 5.2747

>NTDB_id=51058 Q7A_RS07395 WP_014706716.1 1526653..1527756(+) (pilU) [Methylophaga nitratireducenticrescens]
MEMMPYLKLMADKEASDLFFCTATEPQVKIQGVIRPVGSKKMLTGEVKDLAYSLMDQQQIAEFEQNLEMNFAVPVHGVGR
FRVNIFRQRGEVSMVIRYIRTHIPNFEEMSLPPVLGNVVMEKRGLILVVGATGSGKSTTLAAMIGHRNRNSGSHILTIED
PLEFVHSHQRSIVQQREVGIDTLSYENALKNALREAPDVILIGEIRDMDTMKHAINYSETGHLCLATLHANNANQALDRI
INFFPEMMHRQILMDLSLNLRAIISQRLLPSVQDGNLVPAVEVMLSSPYIAELINKGQIGAIKDAMKESSEPGTITFDQA
LLTLYREGKISQEDALRNADSRNDLSLEIRMGASHDDSDAEDELILS

Nucleotide

• Length: 1104 bp

>NTDB_id=51058 Q7A_RS07395 WP_014706716.1 1526653..1527756(+) (pilU) [Methylophaga nitratireducenticrescens]
GTGGAAATGATGCCTTACCTGAAACTGATGGCTGATAAGGAAGCCTCAGATTTGTTTTTTTGTACTGCGACCGAACCGCA
AGTCAAAATTCAAGGGGTGATTCGCCCCGTTGGCAGTAAAAAAATGCTGACAGGTGAAGTGAAAGACCTCGCCTACTCAT
TAATGGATCAGCAACAGATAGCTGAATTTGAACAAAATCTGGAAATGAATTTTGCGGTTCCAGTCCACGGCGTTGGCCGG
TTCCGGGTCAATATCTTCCGGCAGCGTGGCGAAGTCTCCATGGTCATTCGCTATATCCGTACCCATATTCCTAATTTTGA
AGAAATGAGTTTGCCACCTGTACTGGGTAATGTGGTGATGGAAAAACGTGGTTTGATTCTGGTTGTCGGTGCCACCGGCA
GCGGTAAATCCACTACGTTGGCCGCAATGATTGGTCATCGGAATCGTAATTCCGGATCACATATTCTGACCATTGAGGAT
CCGCTGGAATTTGTGCATTCCCATCAACGATCAATTGTGCAGCAACGCGAAGTTGGTATTGACACCCTGTCTTATGAGAA
TGCATTGAAAAATGCCCTGCGTGAAGCGCCAGATGTCATTTTGATTGGTGAGATCCGCGATATGGATACCATGAAACATG
CCATTAATTACTCTGAAACCGGTCACCTGTGTTTAGCCACGTTGCATGCCAATAATGCGAATCAGGCACTGGACCGGATC
ATTAACTTTTTCCCGGAAATGATGCATCGTCAGATCCTGATGGATCTGTCACTGAATTTGCGTGCCATCATCTCGCAGCG
TTTATTACCTTCAGTGCAGGATGGCAATCTGGTACCGGCGGTTGAAGTCATGCTCAGCTCGCCATATATCGCAGAATTGA
TTAATAAAGGTCAGATCGGTGCGATCAAAGATGCGATGAAAGAAAGTAGTGAACCGGGGACGATTACCTTTGACCAGGCC
TTGTTGACATTGTATCGGGAAGGCAAGATTTCTCAGGAAGATGCATTGCGCAATGCCGATTCACGCAATGACCTCAGTCT
GGAAATACGCATGGGAGCATCTCATGATGACAGCGATGCAGAAGACGAATTAATCCTCAGCTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (12-269)

3D structure

• AlphaFold DB: I1XIX4

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	53.933	97.003	0.523
pilU	Vibrio cholerae strain A1552	51.966	97.003	0.504
pilU	Acinetobacter baylyi ADP1	51.275	96.185	0.493
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.544	90.736	0.395
pilT	Neisseria gonorrhoeae MS11	39.368	94.823	0.373
pilT	Neisseria meningitidis 8013	39.368	94.823	0.373
pilT	Vibrio cholerae strain A1552	40.12	91.008	0.365
pilT	Vibrio cholerae O1 biovar El Tor strain E7946	40.12	91.008	0.365
pilT	Pseudomonas stutzeri DSM 10701	39.233	92.371	0.362
pilT	Pseudomonas aeruginosa PAK	39.233	92.371	0.362