Detailed information NTG0051025

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: Q7C_RS01415
• Genome accession: NC_017856
• Coordinates: 291602..292702 (-)
• Length: 366 a.a.
• NCBI ID: WP_041366812.1
• Uniprot ID: -
• Organism: Methylophaga frappieri
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 286602..297702

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
Q7C_RS01395 (Q7C_290)	kdsA	286677..287513 (-)	837	WP_014702919.1	3-deoxy-8-phosphooctulonate synthase	-
Q7C_RS01400 (Q7C_291)	-	287516..289150 (-)	1635	WP_014702920.1	CTP synthase	-
Q7C_RS01405 (Q7C_292)	tilS	289233..290573 (-)	1341	WP_014702921.1	tRNA lysidine(34) synthetase TilS	-
Q7C_RS01410 (Q7C_293)	-	290585..291541 (-)	957	WP_014702922.1	acetyl-CoA carboxylase carboxyltransferase subunit alpha	-
Q7C_RS01415 (Q7C_294)	pilU	291602..292702 (-)	1101	WP_041366812.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
Q7C_RS01420 (Q7C_295)	dnaE	292787..296263 (-)	3477	WP_014702924.1	DNA polymerase III subunit alpha	-
Q7C_RS01425 (Q7C_296)	nagZ	296345..297391 (-)	1047	WP_050954415.1	beta-N-acetylhexosaminidase	-

Sequence

Protein

• Length: 366 a.a.
• Molecular weight: 40804.83 Da
• Isoelectric Point: 5.2739

>NTDB_id=51025 Q7C_RS01415 WP_041366812.1 291602..292702(-) (pilU) [Methylophaga frappieri]
MDMTPYLRLMADKQASDLFFCTGTTPQIKIQGVIRPVGSKNLAPGDVKEMAYSLMNEPQITEFETTLEMNFAVPLSGVGR
FRVNVFKQRGETSMVIRYIRTHIPDFAEMNLPPVLGKVVMEKRGLILVVGSTGSGKSTSLAAMIGYRNQHSNSHILTIED
PLEFVHNHQKSIVQQREVGIDTLSYENALKNALREAPDVILIGEVRDMETMKHAINYAETGHLCLATLHANNANQALDRI
LNFFPEVMHRQLLTDLSLNLRAIISQRLILGNDDLLMPAVEVMLSSPYISELIAKGNISGIKEAMKESSEPGTITFDQAL
LKLYQDGKISEEEALRNADSRNDLSLEIRLNASHDDKDVDNELILS

Nucleotide

• Length: 1101 bp

>NTDB_id=51025 Q7C_RS01415 WP_041366812.1 291602..292702(-) (pilU) [Methylophaga frappieri]
GTGGATATGACTCCTTACTTGAGGCTGATGGCAGATAAACAGGCATCAGACTTGTTTTTTTGTACCGGCACCACACCCCA
AATCAAAATTCAGGGCGTCATTCGTCCTGTGGGCAGCAAAAATCTCGCTCCCGGCGATGTAAAAGAGATGGCTTATTCGC
TCATGAATGAGCCACAAATTACGGAATTTGAAACCACGCTCGAAATGAATTTTGCCGTGCCACTCTCCGGTGTTGGGAGG
TTTCGGGTAAATGTCTTCAAACAGCGTGGCGAAACCTCAATGGTGATTCGTTATATCCGGACACATATTCCTGACTTTGC
TGAAATGAACCTGCCGCCGGTTTTAGGTAAAGTCGTGATGGAAAAGCGTGGCCTGATTTTAGTTGTCGGCTCAACGGGTA
GTGGTAAGTCGACGTCGCTTGCTGCGATGATTGGCTATCGAAATCAGCATAGTAATAGTCATATTCTGACGATTGAAGAT
CCGTTAGAGTTTGTTCACAATCATCAAAAATCCATCGTTCAACAGCGCGAAGTTGGCATTGATACCTTATCTTACGAAAA
TGCCTTGAAAAATGCCTTACGTGAAGCGCCTGATGTCATTTTGATTGGTGAGGTGCGTGACATGGAAACCATGAAGCACG
CAATAAATTACGCAGAGACAGGCCATTTATGCTTGGCAACATTACATGCGAATAATGCAAATCAGGCACTGGATCGAATT
CTGAACTTTTTCCCGGAAGTCATGCATCGGCAGCTACTCACTGATTTGTCATTAAATTTGCGGGCAATTATTTCGCAGCG
TTTAATACTGGGGAATGATGATCTCTTGATGCCTGCGGTAGAGGTGATGTTAAGCAGCCCCTATATTTCGGAACTGATCG
CCAAAGGCAATATTTCCGGTATTAAAGAAGCCATGAAAGAAAGCAGTGAACCTGGCACCATTACCTTTGATCAAGCGCTG
CTTAAACTCTATCAGGACGGTAAAATCAGCGAAGAAGAAGCTCTGCGCAACGCTGATTCACGTAACGACTTGAGTTTGGA
AATTCGACTCAATGCCAGCCACGATGATAAAGATGTGGACAATGAACTGATTCTGAGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (113-269)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	54.775	97.268	0.533
pilU	Acinetobacter baylyi ADP1	51.24	99.18	0.508
pilU	Vibrio cholerae strain A1552	51.404	97.268	0.5
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.988	93.169	0.41
pilT	Pseudomonas aeruginosa PAK	40.828	92.35	0.377
pilT	Vibrio cholerae strain A1552	41.141	90.984	0.374
pilT	Vibrio cholerae O1 biovar El Tor strain E7946	41.141	90.984	0.374
pilT	Neisseria meningitidis 8013	39.481	94.809	0.374
pilT	Neisseria gonorrhoeae MS11	39.481	94.809	0.374
pilT	Pseudomonas stutzeri DSM 10701	39.645	92.35	0.366