Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   I5M77_RS17260 Genome accession   NZ_CP065369
Coordinates   3607216..3607674 (-) Length   152 a.a.
NCBI ID   WP_025518565.1    Uniprot ID   -
Organism   Vibrio parahaemolyticus strain 20-082A3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3602216..3612674
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I5M77_RS17240 (I5M77_17240) coaE 3602728..3603342 (-) 615 WP_005480887.1 dephospho-CoA kinase -
  I5M77_RS17245 (I5M77_17245) pilD 3603343..3604212 (-) 870 WP_005493974.1 prepilin peptidase Machinery gene
  I5M77_RS17250 (I5M77_17250) pilC 3604277..3605500 (-) 1224 WP_005479682.1 type II secretion system F family protein Machinery gene
  I5M77_RS17255 (I5M77_17255) pilB 3605525..3607210 (-) 1686 WP_025792845.1 type IV-A pilus assembly ATPase PilB Machinery gene
  I5M77_RS17260 (I5M77_17260) pilA 3607216..3607674 (-) 459 WP_025518565.1 pilin Machinery gene
  I5M77_RS17265 (I5M77_17265) nadC 3607937..3608824 (-) 888 WP_015297249.1 carboxylating nicotinate-nucleotide diphosphorylase -
  I5M77_RS17270 (I5M77_17270) ampD 3608917..3609468 (+) 552 WP_025792846.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  I5M77_RS17275 (I5M77_17275) pdhR 3609874..3610641 (+) 768 WP_005462576.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 152 a.a.        Molecular weight: 15847.98 Da        Isoelectric Point: 6.9498

>NTDB_id=508907 I5M77_RS17260 WP_025518565.1 3607216..3607674(-) (pilA) [Vibrio parahaemolyticus strain 20-082A3]
MKHSKQKKQQGFTLIELMIVVAVIGVLASIAIPQYQKYVAKSEVASALATLTGVKTNVEAFAVENGKFPDGASTNETEAD
LGVPTTIPSGSITFTAASSSAGTIKFAFDSAGVSNLVTSKNFELVRASDGTWTCQGSSASPVTDDLLPKNCR

Nucleotide


Download         Length: 459 bp        

>NTDB_id=508907 I5M77_RS17260 WP_025518565.1 3607216..3607674(-) (pilA) [Vibrio parahaemolyticus strain 20-082A3]
ATGAAACACAGTAAACAGAAAAAACAGCAAGGTTTTACCTTGATTGAATTGATGATTGTAGTAGCGGTAATTGGTGTTTT
GGCATCTATAGCTATTCCACAGTACCAAAAATACGTTGCGAAGTCCGAGGTAGCATCAGCTCTTGCAACTCTGACTGGCG
TAAAAACTAACGTAGAAGCATTTGCTGTCGAAAATGGTAAATTCCCAGATGGTGCTAGTACAAATGAAACCGAAGCAGAC
TTGGGTGTTCCAACCACTATTCCATCAGGTTCAATAACCTTTACTGCGGCAAGCTCTTCCGCTGGTACAATCAAGTTTGC
GTTTGATAGCGCAGGAGTGAGTAACTTGGTTACTAGTAAAAACTTCGAGCTGGTACGTGCATCTGATGGAACATGGACAT
GTCAAGGTTCTAGCGCAAGCCCGGTAACAGATGATTTGTTACCAAAAAATTGTCGTTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(40-152)

Prokaryotic N-terminal methylation motif (N_methyl)

(7-31)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

48.718

100

0.5
  pilA Vibrio cholerae strain A1552

48.718

100

0.5
  pilA Vibrio cholerae C6706

48.718

100

0.5
  pilA Pseudomonas aeruginosa PAK

42.581

100

0.434
  pilA Acinetobacter baumannii strain A118

42.759

95.395

0.408
  pilA Vibrio parahaemolyticus RIMD 2210633

44.03

88.158

0.388
  pilA/pilAI Pseudomonas stutzeri DSM 10701

40.426

92.763

0.375
  pilA Acinetobacter nosocomialis M2

37.333

98.684

0.368