Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   I5M77_RS17250 Genome accession   NZ_CP065369
Coordinates   3604277..3605500 (-) Length   407 a.a.
NCBI ID   WP_005479682.1    Uniprot ID   Q87LT6
Organism   Vibrio parahaemolyticus strain 20-082A3     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3599277..3610500
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I5M77_RS17210 (I5M77_17210) rpsP 3599405..3599653 (+) 249 WP_005379962.1 30S ribosomal protein S16 -
  I5M77_RS17215 (I5M77_17215) rimM 3599682..3600230 (+) 549 WP_005462552.1 ribosome maturation factor RimM -
  I5M77_RS17220 (I5M77_17220) trmD 3600258..3601001 (+) 744 WP_005462562.1 tRNA (guanosine(37)-N1)-methyltransferase TrmD -
  I5M77_RS17225 (I5M77_17225) rplS 3601043..3601396 (+) 354 WP_005462554.1 50S ribosomal protein L19 -
  I5M77_RS17230 (I5M77_17230) yacG 3601697..3601891 (-) 195 WP_005462546.1 DNA gyrase inhibitor YacG -
  I5M77_RS17235 (I5M77_17235) zapD 3601960..3602700 (-) 741 WP_015297254.1 cell division protein ZapD -
  I5M77_RS17240 (I5M77_17240) coaE 3602728..3603342 (-) 615 WP_005480887.1 dephospho-CoA kinase -
  I5M77_RS17245 (I5M77_17245) pilD 3603343..3604212 (-) 870 WP_005493974.1 prepilin peptidase Machinery gene
  I5M77_RS17250 (I5M77_17250) pilC 3604277..3605500 (-) 1224 WP_005479682.1 type II secretion system F family protein Machinery gene
  I5M77_RS17255 (I5M77_17255) pilB 3605525..3607210 (-) 1686 WP_025792845.1 type IV-A pilus assembly ATPase PilB Machinery gene
  I5M77_RS17260 (I5M77_17260) pilA 3607216..3607674 (-) 459 WP_025518565.1 pilin Machinery gene
  I5M77_RS17265 (I5M77_17265) nadC 3607937..3608824 (-) 888 WP_015297249.1 carboxylating nicotinate-nucleotide diphosphorylase -
  I5M77_RS17270 (I5M77_17270) ampD 3608917..3609468 (+) 552 WP_025792846.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -

Sequence

Protein


Download         Length: 407 a.a.        Molecular weight: 45220.39 Da        Isoelectric Point: 10.4368

>NTDB_id=508905 I5M77_RS17250 WP_005479682.1 3604277..3605500(-) (pilC) [Vibrio parahaemolyticus strain 20-082A3]
MKTTTPQLKNFRWKGVNSSGKKTSGQTLAMSEIEVRERLDAQHIKIKKLKKSSISFLTKLSHRVKGKDITVFTRQISTML
VTGVPLVQALKLVSDNHKKAEMKSILMSVTRAVEAGTPMSKAMRTASNHFDPLYTDLIATGEQSGNLAEVFERLATYREK
NEQLRAKVIKALIYPAMVILVALGVSFIMLTKVIPEFEKMFVGFGAELPWFTRQVLDLSAWTQNWSPFIALGSISVFISA
RVLSKRSDSFRLMLNRSVLKFPVLGAVLSKAAIAKFSRTLATSFTAGIPILTSLKTTSKTSGNMHYQLAIEEVYRDTAAG
MPMYVAMRNCNVFPELVLQMVMIGEESGRLDDMLNKVATIYEFEVDNTVDNLSKILEPLIIVFLGIVVGGLVTAMYLPIF
NLMSVLG

Nucleotide


Download         Length: 1224 bp        

>NTDB_id=508905 I5M77_RS17250 WP_005479682.1 3604277..3605500(-) (pilC) [Vibrio parahaemolyticus strain 20-082A3]
ATGAAAACTACGACACCACAATTAAAGAACTTCCGTTGGAAAGGCGTCAACAGCTCAGGGAAAAAGACCTCTGGTCAAAC
TCTGGCAATGAGCGAAATCGAAGTACGAGAGCGCTTAGATGCCCAGCACATCAAAATCAAAAAGCTAAAGAAAAGCAGCA
TCTCTTTTTTGACCAAACTTAGCCATCGCGTAAAAGGGAAAGACATCACGGTTTTCACTCGTCAAATATCAACGATGCTG
GTAACCGGTGTGCCGCTAGTACAAGCATTGAAATTGGTCTCTGATAACCACAAAAAAGCGGAAATGAAATCCATTTTGAT
GAGTGTAACTCGGGCTGTTGAAGCGGGTACGCCGATGTCCAAAGCGATGCGTACCGCCAGTAACCATTTTGACCCGCTGT
ATACCGACCTGATTGCGACAGGAGAACAATCGGGCAACTTAGCCGAAGTGTTCGAACGCTTAGCCACCTACAGGGAAAAA
AATGAGCAGCTTAGAGCCAAAGTGATCAAGGCTCTAATATATCCAGCCATGGTTATTCTAGTTGCACTGGGAGTATCGTT
TATCATGCTAACCAAGGTCATTCCCGAGTTTGAAAAAATGTTTGTTGGCTTTGGCGCTGAATTGCCATGGTTTACAAGGC
AAGTCTTAGATCTTTCCGCTTGGACACAAAACTGGAGCCCATTTATCGCACTTGGCTCCATCAGTGTATTTATCTCCGCG
AGAGTACTCTCTAAGCGTTCAGATTCTTTTCGCTTAATGCTCAACCGCTCTGTGCTTAAGTTTCCAGTCCTTGGGGCAGT
TTTATCGAAAGCCGCTATCGCAAAATTTAGTCGAACCCTCGCTACGAGCTTTACGGCGGGCATTCCGATTTTAACCAGTT
TAAAAACCACCTCGAAAACGTCCGGAAATATGCATTATCAATTGGCTATCGAAGAGGTTTATCGCGATACCGCAGCAGGA
ATGCCGATGTATGTTGCAATGCGCAATTGCAACGTGTTCCCTGAGTTGGTGCTGCAAATGGTTATGATCGGCGAAGAATC
CGGCCGACTTGACGATATGCTCAATAAAGTCGCCACAATTTACGAGTTTGAAGTGGACAACACCGTCGATAACCTCAGTA
AAATCTTAGAACCATTAATTATCGTTTTTTTAGGTATCGTTGTCGGTGGCCTAGTCACAGCAATGTACTTGCCAATCTTT
AACTTAATGAGTGTATTGGGCTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(276-398)

Type II secretion system (T2SS), protein F (T2SSF)

(72-195)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q87LT6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Vibrio campbellii strain DS40M4

85.995

100

0.86
  pilC Vibrio cholerae strain A1552

74.321

99.509

0.74
  pilC Acinetobacter baylyi ADP1

42.75

98.28

0.42
  pilC Acinetobacter baumannii D1279779

41.439

99.017

0.41
  pilG Neisseria gonorrhoeae MS11

40.494

99.509

0.403
  pilG Neisseria meningitidis 44/76-A

40.247

99.509

0.4
  pilC Pseudomonas stutzeri DSM 10701

40.909

97.297

0.398
  pilC Legionella pneumophila strain ERS1305867

39.803

100

0.398