Detailed information NTG0508904

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: I5M77_RS17245
• Genome accession: NZ_CP065369
• Coordinates: 3603343..3604212 (-)
• Length: 289 a.a.
• NCBI ID: WP_005493974.1
• Uniprot ID: A0A249W3M2
• Organism: Vibrio parahaemolyticus strain 20-082A3
• Function: type IV pilus biogenesis and function (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3598343..3609212

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
I5M77_RS17210 (I5M77_17210)	rpsP	3599405..3599653 (+)	249	WP_005379962.1	30S ribosomal protein S16	-
I5M77_RS17215 (I5M77_17215)	rimM	3599682..3600230 (+)	549	WP_005462552.1	ribosome maturation factor RimM	-
I5M77_RS17220 (I5M77_17220)	trmD	3600258..3601001 (+)	744	WP_005462562.1	tRNA (guanosine(37)-N1)-methyltransferase TrmD	-
I5M77_RS17225 (I5M77_17225)	rplS	3601043..3601396 (+)	354	WP_005462554.1	50S ribosomal protein L19	-
I5M77_RS17230 (I5M77_17230)	yacG	3601697..3601891 (-)	195	WP_005462546.1	DNA gyrase inhibitor YacG	-
I5M77_RS17235 (I5M77_17235)	zapD	3601960..3602700 (-)	741	WP_015297254.1	cell division protein ZapD	-
I5M77_RS17240 (I5M77_17240)	coaE	3602728..3603342 (-)	615	WP_005480887.1	dephospho-CoA kinase	-
I5M77_RS17245 (I5M77_17245)	pilD	3603343..3604212 (-)	870	WP_005493974.1	prepilin peptidase	Machinery gene
I5M77_RS17250 (I5M77_17250)	pilC	3604277..3605500 (-)	1224	WP_005479682.1	type II secretion system F family protein	Machinery gene
I5M77_RS17255 (I5M77_17255)	pilB	3605525..3607210 (-)	1686	WP_025792845.1	type IV-A pilus assembly ATPase PilB	Machinery gene
I5M77_RS17260 (I5M77_17260)	pilA	3607216..3607674 (-)	459	WP_025518565.1	pilin	Machinery gene
I5M77_RS17265 (I5M77_17265)	nadC	3607937..3608824 (-)	888	WP_015297249.1	carboxylating nicotinate-nucleotide diphosphorylase	-

Sequence

Protein

• Length: 289 a.a.
• Molecular weight: 32377.43 Da
• Isoelectric Point: 7.1971

>NTDB_id=508904 I5M77_RS17245 WP_005493974.1 3603343..3604212(-) (pilD) [Vibrio parahaemolyticus strain 20-082A3]
MEVFQYYPWLFVVFASIFGLIVGSFLNVVIYRLPKIMELEWRRECAESFPEYKIKPPQEVLTLSVPRSSCQNCATPIRIR
DNIPVISWLLLKGKCHHCHTAISPRYPLIELLTAACAGFVAYHFGFSYFTVALIFFTFVLIAATFIDLDTMLLPDQLTLP
LTWAGIALALTEISPVSLQDAVIGAIAGYLCLWSVYWGFKLLTGKEGMGYGDFKLLAALGAWLGWQSLPMIILLSSVVGV
IFGLVQLRLQKQGIERAFPFGPYLAIAGWASLIWGDQILSWYFTSILGV

Nucleotide

• Length: 870 bp

>NTDB_id=508904 I5M77_RS17245 WP_005493974.1 3603343..3604212(-) (pilD) [Vibrio parahaemolyticus strain 20-082A3]
ATGGAAGTGTTCCAGTACTACCCTTGGCTATTCGTGGTGTTTGCCAGTATCTTCGGTTTAATTGTGGGTAGTTTTCTTAA
TGTAGTCATTTATCGGCTACCTAAAATCATGGAATTAGAATGGCGCCGTGAATGCGCCGAATCATTCCCTGAATATAAGA
TTAAGCCACCTCAAGAAGTTTTGACATTAAGCGTGCCTCGCTCTTCGTGCCAAAACTGCGCGACGCCAATTCGTATTCGA
GACAATATTCCTGTAATCAGCTGGCTACTGTTAAAAGGAAAATGCCATCATTGTCATACCGCAATTAGTCCTCGATATCC
GCTGATAGAATTACTCACTGCAGCGTGCGCTGGTTTTGTGGCTTATCATTTCGGCTTTAGCTACTTTACGGTTGCGTTAA
TCTTTTTCACATTTGTCTTAATAGCCGCGACTTTTATCGATCTCGATACTATGCTGCTACCGGACCAATTAACATTACCA
TTAACTTGGGCTGGCATTGCGTTAGCATTAACAGAAATAAGCCCTGTTAGTCTACAAGACGCCGTAATTGGTGCGATTGC
CGGTTATTTGTGTCTTTGGTCAGTATATTGGGGTTTCAAACTGCTTACTGGTAAAGAAGGCATGGGTTACGGTGATTTTA
AATTGCTGGCTGCCCTTGGCGCTTGGCTAGGATGGCAATCATTACCAATGATCATCCTACTTTCTTCCGTCGTCGGTGTG
ATATTCGGGCTCGTTCAGTTACGACTACAAAAACAAGGTATAGAGAGAGCTTTCCCTTTCGGCCCATATTTAGCGATTGC
AGGATGGGCAAGCTTAATCTGGGGCGATCAAATTCTAAGTTGGTACTTCACGTCAATTTTAGGAGTGTAA

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (135-244)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (17-124)

3D structure

• AlphaFold DB: A0A249W3M2

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio campbellii strain DS40M4	88.927	100	0.889
pilD	Vibrio cholerae strain A1552	73.611	99.654	0.734
pilD	Acinetobacter baumannii D1279779	52.239	92.734	0.484
pilD	Acinetobacter nosocomialis M2	51.866	92.734	0.481
pilD	Neisseria gonorrhoeae MS11	47.101	95.502	0.45