Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   I3X05_RS13645 Genome accession   NZ_CP065217
Coordinates   2912480..2914168 (+) Length   562 a.a.
NCBI ID   WP_337970802.1    Uniprot ID   A0AAJ4LTS9
Organism   Vibrio navarrensis strain 20-VB00237     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2907480..2919168
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I3X05_RS13625 (I3X05_13970) pdhR 2909093..2909863 (-) 771 WP_039430445.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  I3X05_RS13630 (I3X05_13975) ampD 2910273..2910836 (-) 564 WP_337970800.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  I3X05_RS13635 (I3X05_13980) nadC 2910929..2911816 (+) 888 WP_337970801.1 carboxylating nicotinate-nucleotide diphosphorylase -
  I3X05_RS13640 (I3X05_13985) - 2912032..2912472 (+) 441 WP_045571203.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  I3X05_RS13645 (I3X05_13990) pilB 2912480..2914168 (+) 1689 WP_337970802.1 type IV-A pilus assembly ATPase PilB Machinery gene
  I3X05_RS13650 (I3X05_13995) pilC 2914189..2915415 (+) 1227 WP_193185744.1 type II secretion system F family protein Machinery gene
  I3X05_RS13655 (I3X05_14000) pilD 2915555..2916424 (+) 870 WP_193157540.1 A24 family peptidase Machinery gene
  I3X05_RS13660 (I3X05_14005) coaE 2916426..2917034 (+) 609 WP_193167015.1 dephospho-CoA kinase -
  I3X05_RS13665 (I3X05_14010) zapD 2917067..2917807 (+) 741 WP_045571208.1 cell division protein ZapD -
  I3X05_RS13670 (I3X05_14015) yacG 2917891..2918085 (+) 195 WP_045571209.1 DNA gyrase inhibitor YacG -
  I3X05_RS13675 (I3X05_14020) rplS 2918378..2918731 (-) 354 WP_045571210.1 50S ribosomal protein L19 -

Sequence

Protein


Download         Length: 562 a.a.        Molecular weight: 62154.26 Da        Isoelectric Point: 6.4369

>NTDB_id=508202 I3X05_RS13645 WP_337970802.1 2912480..2914168(+) (pilB) [Vibrio navarrensis strain 20-VB00237]
MTNNLVIILRQSNLLSSTQEQACLEHLNASGASAPEVLLQLGFFQAEELTEHLSALFGLPPVELSHYDYQSVCNQLGLRE
LITRHQALPLQKNRSALILASADPTNLQAEDDFRFATGLQVEMVLADVKQLQGAIRRLYGRTLAQDNRSGLKEIHQDELA
NLVNVAADEVDNIEDLSQDSSPVSRFINQILLDAVRKGASDIHFEPYEAMYRVRLRCDGILVEVQQPPSHLSRRLSARLK
ILAKLDIAERRLPQDGRIKLKLNQSTAIDMRVSTLPTLFGEKIVLRLLDSSSSNLDIDKLGYSETQKQLYLNALRKPQGM
ILMTGPTGSGKTVSLYTGLSLLNRPEVNISTAEDPVEINLPGINQVQVQPKIGFGFAQALRSFLRQDPDIVMVGEIRDLE
TAEIAIKAAQTGHLVLSTLHTNSAAETIVRLSNMGIASFNLASSLSLIIAQRLARRLCRHCKQPQHLSPALQELGLNQQD
GLYRANPQGCNECNQGYSGRVGIYEVMPFDDAMAEALVKGASVQSLEMLARKNGMLTLRESGLEKVKQGITSIEELQRVL
YL

Nucleotide


Download         Length: 1689 bp        

>NTDB_id=508202 I3X05_RS13645 WP_337970802.1 2912480..2914168(+) (pilB) [Vibrio navarrensis strain 20-VB00237]
ATGACTAACAATCTTGTAATTATCCTTCGTCAATCTAATTTACTTAGCTCAACGCAAGAGCAAGCCTGTTTGGAACATCT
CAATGCTTCCGGTGCCAGTGCACCGGAGGTGTTGTTGCAACTGGGCTTTTTCCAAGCCGAAGAACTGACTGAACATCTCA
GCGCGCTGTTTGGCTTGCCGCCCGTCGAGCTTAGCCACTATGACTACCAAAGCGTGTGCAACCAACTGGGGTTGCGCGAG
CTTATCACCCGCCACCAAGCCCTGCCGCTACAAAAAAATCGCTCAGCGCTAATTCTCGCCAGTGCCGACCCAACCAATTT
ACAGGCCGAAGATGACTTTCGCTTTGCCACAGGGCTGCAAGTGGAAATGGTCTTGGCCGATGTTAAGCAACTGCAAGGGG
CAATACGTCGCCTATATGGCCGCACGCTTGCTCAAGATAACCGCTCAGGATTGAAAGAGATCCACCAAGATGAGCTAGCC
AACTTAGTCAATGTGGCAGCCGATGAAGTCGATAATATTGAAGATCTCAGCCAAGACAGCTCCCCTGTCAGCCGTTTTAT
CAACCAGATCTTACTCGATGCGGTGCGTAAAGGCGCATCCGACATTCACTTTGAACCCTATGAAGCGATGTATCGCGTGC
GTTTGCGCTGCGATGGTATTTTGGTCGAAGTGCAGCAACCACCGAGTCACTTGAGCCGTCGACTCTCCGCACGGCTTAAG
ATTCTCGCCAAGCTGGATATCGCCGAGCGGCGTTTACCGCAAGATGGGCGCATCAAACTCAAACTGAACCAAAGCACCGC
GATTGATATGCGCGTTTCTACCCTACCGACACTGTTTGGCGAAAAAATCGTGCTGCGTCTGCTCGACAGTTCCAGCAGCA
ACTTAGACATTGATAAGCTCGGCTACAGCGAAACGCAAAAGCAGCTCTATCTCAACGCGCTGCGCAAACCTCAAGGGATG
ATCTTGATGACGGGCCCAACCGGCAGCGGCAAAACCGTTTCACTCTACACCGGTTTAAGCCTACTCAATCGCCCTGAGGT
CAACATCTCTACCGCCGAAGATCCGGTAGAGATTAACTTGCCCGGCATTAACCAAGTGCAAGTACAGCCAAAAATCGGCT
TTGGCTTTGCGCAGGCGCTGCGCTCATTTTTACGTCAAGACCCCGATATTGTCATGGTCGGTGAAATTCGTGATTTAGAA
ACTGCCGAAATAGCCATTAAAGCCGCGCAAACAGGGCATTTAGTGCTATCCACCCTACATACCAACTCGGCCGCAGAGAC
CATAGTACGCCTATCAAACATGGGCATTGCCAGCTTCAACCTCGCTTCTTCATTGAGCTTAATTATCGCTCAGCGCCTAG
CGCGTCGTTTGTGTCGCCATTGCAAGCAGCCACAACACCTTTCACCTGCATTGCAAGAGCTGGGTTTAAACCAGCAGGAT
GGGCTGTATCGCGCCAATCCTCAAGGATGTAACGAATGCAATCAAGGGTATTCAGGGCGCGTCGGCATCTACGAAGTGAT
GCCCTTTGACGATGCGATGGCCGAGGCGCTGGTTAAAGGAGCCTCAGTACAAAGCTTGGAAATGCTTGCCAGAAAAAATG
GCATGCTGACGCTTCGAGAATCCGGGCTGGAAAAAGTCAAACAAGGCATCACCAGCATTGAAGAGCTGCAACGCGTGCTC
TACCTGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(189-456)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(38-142)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio parahaemolyticus RIMD 2210633

77.402

100

0.774
  pilB Vibrio campbellii strain DS40M4

76.157

100

0.762
  pilB Vibrio cholerae strain A1552

72.824

100

0.73
  pilB Acinetobacter baumannii D1279779

52.713

91.815

0.484
  pilB Legionella pneumophila strain ERS1305867

51.233

93.772

0.48
  pilB Acinetobacter baylyi ADP1

50.581

91.815

0.464
  pilF Neisseria gonorrhoeae MS11

45.246

100

0.457
  pilB/pilB1 Synechocystis sp. PCC 6803

38.648

100

0.397
  pilF Thermus thermophilus HB27

39.286

99.644

0.391
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

37.011

100

0.37
  pilB Haemophilus influenzae 86-028NP

42.623

86.833

0.37
  pilB Haemophilus influenzae Rd KW20

42.975

86.121

0.37