Detailed information
Bioinformatically predicted
Overview
| Name | pilA | Type | Machinery gene |
| Locus tag | ITG08_RS03180 | Genome accession | NZ_CP065206 |
| Coordinates | 726672..727097 (+) | Length | 141 a.a. |
| NCBI ID | WP_016796129.1 | Uniprot ID | - |
| Organism | Vibrio cyclitrophicus strain ED653 | ||
| Function | assembly of type IV pilus (predicted from homology) DNA binding and uptake |
||
Related MGE
Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.
Gene-MGE association summary
| MGE type | MGE coordinates | Gene coordinates | Relative position | Distance (bp) |
|---|---|---|---|---|
| Genomic island | 718952..734923 | 726672..727097 | within | 0 |
Gene organization within MGE regions
Location: 718952..734923
| Locus tag | Gene name | Coordinates (strand) | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| ITG08_RS03155 (ITG08_03145) | aceF | 718952..720844 (-) | 1893 | WP_248370540.1 | pyruvate dehydrogenase complex dihydrolipoyllysine-residue acetyltransferase | - |
| ITG08_RS03160 (ITG08_03150) | aceE | 720875..723538 (-) | 2664 | WP_016787824.1 | pyruvate dehydrogenase (acetyl-transferring), homodimeric type | - |
| ITG08_RS03165 (ITG08_03155) | pdhR | 723601..724362 (-) | 762 | WP_016768209.1 | pyruvate dehydrogenase complex transcriptional repressor PdhR | - |
| ITG08_RS03170 (ITG08_03160) | ampD | 724844..725443 (-) | 600 | WP_016796130.1 | 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD | - |
| ITG08_RS03175 (ITG08_03165) | nadC | 725527..726417 (+) | 891 | WP_004738163.1 | carboxylating nicotinate-nucleotide diphosphorylase | - |
| ITG08_RS03180 (ITG08_03170) | pilA | 726672..727097 (+) | 426 | WP_016796129.1 | pilin | Machinery gene |
| ITG08_RS03185 (ITG08_03175) | pilB | 727102..728787 (+) | 1686 | WP_029189239.1 | type IV-A pilus assembly ATPase PilB | Machinery gene |
| ITG08_RS03190 (ITG08_03180) | pilC | 728802..730031 (+) | 1230 | WP_016768204.1 | type II secretion system F family protein | Machinery gene |
| ITG08_RS03195 (ITG08_03185) | pilD | 730098..730967 (+) | 870 | WP_016768203.1 | A24 family peptidase | Machinery gene |
| ITG08_RS03200 (ITG08_03190) | coaE | 730970..731584 (+) | 615 | WP_010435920.1 | dephospho-CoA kinase | - |
| ITG08_RS03205 (ITG08_03195) | zapD | 731611..732351 (+) | 741 | WP_010435917.1 | cell division protein ZapD | - |
| ITG08_RS03210 (ITG08_03200) | yacG | 732433..732630 (+) | 198 | WP_010435916.1 | DNA gyrase inhibitor YacG | - |
| ITG08_RS03215 (ITG08_03205) | rplS | 732916..733269 (-) | 354 | WP_004735514.1 | 50S ribosomal protein L19 | - |
| ITG08_RS03220 (ITG08_03210) | trmD | 733329..734069 (-) | 741 | WP_010435912.1 | tRNA (guanosine(37)-N1)-methyltransferase TrmD | - |
| ITG08_RS03225 (ITG08_03215) | rimM | 734096..734650 (-) | 555 | WP_016768202.1 | ribosome maturation factor RimM | - |
| ITG08_RS03230 (ITG08_03220) | rpsP | 734675..734923 (-) | 249 | WP_004410028.1 | 30S ribosomal protein S16 | - |
Sequence
Protein
Download Length: 141 a.a. Molecular weight: 14672.71 Da Isoelectric Point: 4.3321
>NTDB_id=507996 ITG08_RS03180 WP_016796129.1 726672..727097(+) (pilA) [Vibrio cyclitrophicus strain ED653]
MNNKSKRTNQKGFTLIELMIVVAIIGVLSAIAVPAYKDYVAKSQASSALATLKSLVTPAELFIQENGDLTTLDELGIASG
ANSLGEIKIATQAASSAEISFTFADGALDTGVIAVTRSDTGWACTRTVSSLLQSVELEGCD
MNNKSKRTNQKGFTLIELMIVVAIIGVLSAIAVPAYKDYVAKSQASSALATLKSLVTPAELFIQENGDLTTLDELGIASG
ANSLGEIKIATQAASSAEISFTFADGALDTGVIAVTRSDTGWACTRTVSSLLQSVELEGCD
Nucleotide
Download Length: 426 bp
>NTDB_id=507996 ITG08_RS03180 WP_016796129.1 726672..727097(+) (pilA) [Vibrio cyclitrophicus strain ED653]
ATGAATAACAAAAGTAAAAGAACAAATCAGAAAGGTTTCACACTGATTGAATTGATGATTGTGGTAGCGATTATTGGCGT
GTTGTCTGCGATTGCTGTTCCTGCATATAAAGACTACGTAGCCAAAAGCCAAGCATCTTCAGCTTTAGCCACATTAAAAT
CTCTAGTAACACCTGCGGAGCTATTTATTCAAGAAAATGGCGACTTAACAACTCTAGACGAACTCGGAATAGCTTCAGGA
GCCAATTCTCTTGGAGAAATAAAAATAGCAACTCAAGCTGCATCTTCTGCAGAAATATCTTTTACCTTTGCTGATGGCGC
GTTAGACACTGGGGTTATTGCTGTGACGCGGTCAGATACAGGCTGGGCTTGTACTCGAACAGTGAGCTCTCTCCTTCAAT
CTGTTGAACTAGAAGGTTGTGATTAA
ATGAATAACAAAAGTAAAAGAACAAATCAGAAAGGTTTCACACTGATTGAATTGATGATTGTGGTAGCGATTATTGGCGT
GTTGTCTGCGATTGCTGTTCCTGCATATAAAGACTACGTAGCCAAAAGCCAAGCATCTTCAGCTTTAGCCACATTAAAAT
CTCTAGTAACACCTGCGGAGCTATTTATTCAAGAAAATGGCGACTTAACAACTCTAGACGAACTCGGAATAGCTTCAGGA
GCCAATTCTCTTGGAGAAATAAAAATAGCAACTCAAGCTGCATCTTCTGCAGAAATATCTTTTACCTTTGCTGATGGCGC
GTTAGACACTGGGGTTATTGCTGTGACGCGGTCAGATACAGGCTGGGCTTGTACTCGAACAGTGAGCTCTCTCCTTCAAT
CTGTTGAACTAGAAGGTTGTGATTAA
3D structure
| Source | ID | Structure |
|---|
Similar proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| pilA | Vibrio parahaemolyticus RIMD 2210633 |
50 |
100 |
0.504 |
| pilA | Pseudomonas aeruginosa PAK |
43.609 |
94.326 |
0.411 |
| pilA | Vibrio cholerae C6706 |
39.726 |
100 |
0.411 |
| pilA | Vibrio cholerae O1 biovar El Tor strain E7946 |
39.726 |
100 |
0.411 |
| pilA | Vibrio cholerae strain A1552 |
39.726 |
100 |
0.411 |
| comP | Acinetobacter baylyi ADP1 |
36.806 |
100 |
0.376 |
| pilA | Acinetobacter baumannii strain A118 |
38.235 |
96.454 |
0.369 |
| pilA/pilAII | Pseudomonas stutzeri DSM 10701 |
36.957 |
97.872 |
0.362 |
| pilA/pilAI | Pseudomonas stutzeri DSM 10701 |
38.06 |
95.035 |
0.362 |