Detailed information NTG0507997

Bioinformatically predicted

Overview

• Name: pilB
• Type: Machinery gene
• Locus tag: ITG08_RS03185
• Genome accession: NZ_CP065206
• Coordinates: 727102..728787 (+)
• Length: 561 a.a.
• NCBI ID: WP_029189239.1
• Uniprot ID: -
• Organism: Vibrio cyclitrophicus strain ED653
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
Genomic island	718952..734923	727102..728787	within	0

Gene organization within MGE regions

Location: 718952..734923

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ITG08_RS03155 (ITG08_03145)	aceF	718952..720844 (-)	1893	WP_248370540.1	pyruvate dehydrogenase complex dihydrolipoyllysine-residue acetyltransferase	-
ITG08_RS03160 (ITG08_03150)	aceE	720875..723538 (-)	2664	WP_016787824.1	pyruvate dehydrogenase (acetyl-transferring), homodimeric type	-
ITG08_RS03165 (ITG08_03155)	pdhR	723601..724362 (-)	762	WP_016768209.1	pyruvate dehydrogenase complex transcriptional repressor PdhR	-
ITG08_RS03170 (ITG08_03160)	ampD	724844..725443 (-)	600	WP_016796130.1	1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD	-
ITG08_RS03175 (ITG08_03165)	nadC	725527..726417 (+)	891	WP_004738163.1	carboxylating nicotinate-nucleotide diphosphorylase	-
ITG08_RS03180 (ITG08_03170)	pilA	726672..727097 (+)	426	WP_016796129.1	pilin	Machinery gene
ITG08_RS03185 (ITG08_03175)	pilB	727102..728787 (+)	1686	WP_029189239.1	type IV-A pilus assembly ATPase PilB	Machinery gene
ITG08_RS03190 (ITG08_03180)	pilC	728802..730031 (+)	1230	WP_016768204.1	type II secretion system F family protein	Machinery gene
ITG08_RS03195 (ITG08_03185)	pilD	730098..730967 (+)	870	WP_016768203.1	A24 family peptidase	Machinery gene
ITG08_RS03200 (ITG08_03190)	coaE	730970..731584 (+)	615	WP_010435920.1	dephospho-CoA kinase	-
ITG08_RS03205 (ITG08_03195)	zapD	731611..732351 (+)	741	WP_010435917.1	cell division protein ZapD	-
ITG08_RS03210 (ITG08_03200)	yacG	732433..732630 (+)	198	WP_010435916.1	DNA gyrase inhibitor YacG	-
ITG08_RS03215 (ITG08_03205)	rplS	732916..733269 (-)	354	WP_004735514.1	50S ribosomal protein L19	-
ITG08_RS03220 (ITG08_03210)	trmD	733329..734069 (-)	741	WP_010435912.1	tRNA (guanosine(37)-N1)-methyltransferase TrmD	-
ITG08_RS03225 (ITG08_03215)	rimM	734096..734650 (-)	555	WP_016768202.1	ribosome maturation factor RimM	-
ITG08_RS03230 (ITG08_03220)	rpsP	734675..734923 (-)	249	WP_004410028.1	30S ribosomal protein S16	-

Sequence

Protein

• Length: 561 a.a.
• Molecular weight: 62110.74 Da
• Isoelectric Point: 5.1241

>NTDB_id=507997 ITG08_RS03185 WP_029189239.1 727102..728787(+) (pilB) [Vibrio cyclitrophicus strain ED653]
MLTNLPTVLRQADLLSLTQEQAVAEHVQASGISTPEALLVLDFFSGDSLANNIKTIFGLPLVRLASTDYESLCEQLGLRE
LITKYRAIPISVSSSTLTLASADPTDLQAEDDFRFATGLQVELVVANYSELEGAIRKLYGRSISGQDSKRKEITQDELAN
LVKVSDDEITSIEDLSQDDSPVSRFINQILVDAVRKGASDIHFEPYEEHYRVRLRCDGILVETQQPASHLSRRLSARLKI
LAKLDIAERRLPQDGRIKLRLNDELAIDMRVSTLPTLWGEKIVLRLLDSSAANLDIDKLGYSEAQKALYLNALKRPQGMI
LMTGPTGSGKTVSLYTGLRVLNTSERNISTAEDPVEINLCGINQVQVTPKIGFGFAEALRSFLRQDPDIVMVGEIRDLET
AEIAIKASQTGHLVLSTLHTNSAAETVTRLAHMGIEPFNLASSLSLIIAQRLARRLCNHCKETDDSPDIFLRHSIPNSQT
IYKANSQGCNECNQGYSGRVGIYEVMPFTDNLKTSLINKPNALAIEDLARREGMRTLQESGLDKLLEGTTSYQELQRVLY
L

Nucleotide

• Length: 1686 bp

>NTDB_id=507997 ITG08_RS03185 WP_029189239.1 727102..728787(+) (pilB) [Vibrio cyclitrophicus strain ED653]
GTGCTAACCAACCTCCCAACCGTTCTACGTCAGGCTGATTTACTTAGCCTGACTCAGGAACAAGCCGTGGCGGAACATGT
ACAAGCTTCAGGTATTTCGACACCTGAAGCTTTACTCGTTCTGGATTTTTTCAGCGGCGATTCCTTAGCAAACAACATTA
AAACCATCTTCGGCTTACCGTTAGTACGATTAGCCAGTACTGACTACGAATCCCTGTGTGAACAATTAGGGCTTCGTGAA
CTGATTACTAAGTATCGTGCTATTCCAATTTCAGTTTCTAGCTCAACTCTCACACTCGCTTCTGCCGACCCTACGGATTT
ACAGGCTGAAGATGACTTCCGCTTTGCGACAGGGTTGCAGGTTGAATTGGTTGTTGCTAATTACTCTGAATTAGAAGGCG
CGATACGCAAGCTGTATGGTCGATCTATTTCCGGGCAAGATTCTAAGCGTAAAGAGATCACCCAAGATGAGCTAGCTAAC
CTCGTGAAGGTATCTGACGACGAAATAACGTCGATTGAAGATCTCAGCCAAGACGACTCCCCTGTGAGTCGCTTTATCAA
TCAGATTCTGGTTGATGCGGTACGTAAAGGCGCATCGGACATCCATTTTGAGCCCTACGAAGAACACTACCGAGTGCGCC
TACGTTGCGATGGCATTCTTGTCGAGACCCAACAGCCCGCCTCTCATTTAAGCCGTCGATTATCAGCGCGCTTAAAGATC
CTCGCAAAACTGGATATTGCAGAACGTCGCTTACCTCAAGATGGTCGTATTAAACTGCGTTTAAATGACGAACTGGCAAT
TGATATGCGCGTATCAACACTGCCTACCTTATGGGGCGAGAAGATCGTACTGCGATTGCTTGATAGCAGTGCGGCCAATC
TCGATATCGATAAGCTCGGTTACAGCGAGGCGCAAAAAGCCCTCTACCTCAATGCACTAAAGCGCCCGCAAGGGATGATC
TTAATGACCGGGCCAACCGGCAGCGGTAAAACGGTTTCTCTTTATACTGGACTTCGAGTACTCAACACCTCTGAACGCAA
CATCTCAACAGCTGAAGACCCTGTAGAGATTAACCTGTGTGGCATCAATCAGGTACAAGTGACGCCTAAGATTGGCTTTG
GTTTCGCCGAGGCACTACGATCGTTTTTACGACAAGACCCAGACATCGTGATGGTTGGAGAAATCCGAGATTTAGAAACC
GCCGAGATCGCGATCAAAGCATCGCAAACAGGTCACTTGGTGCTTTCTACACTGCACACCAATTCTGCTGCTGAAACCGT
AACTCGGCTTGCTCACATGGGAATTGAACCCTTTAATCTTGCTTCGTCATTGAGCCTAATCATAGCCCAACGACTGGCAA
GACGCTTGTGCAACCATTGCAAAGAAACAGACGACTCGCCGGACATATTTCTGCGTCACTCGATTCCAAACAGCCAAACC
ATCTACAAAGCCAACTCTCAAGGCTGTAATGAGTGTAACCAAGGTTACTCAGGTCGAGTGGGTATCTATGAAGTGATGCC
GTTTACCGACAACCTAAAAACCAGCCTTATCAATAAACCCAATGCGCTAGCGATTGAAGATCTCGCACGTCGAGAAGGCA
TGCGCACTCTGCAAGAGTCAGGGCTAGATAAACTGCTTGAAGGCACCACCAGCTATCAAGAGCTACAACGTGTTCTATAC
CTATAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (188-455)

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (43-141)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilB	Vibrio cholerae strain A1552	74.113	100	0.745
pilB	Vibrio parahaemolyticus RIMD 2210633	70.819	100	0.709
pilB	Vibrio campbellii strain DS40M4	70.641	100	0.708
pilB	Legionella pneumophila strain ERS1305867	48.007	98.396	0.472
pilB	Acinetobacter baumannii D1279779	50.674	92.513	0.469
pilB	Acinetobacter baylyi ADP1	49.419	91.979	0.455
pilF	Neisseria gonorrhoeae MS11	44.324	98.93	0.438
pilF	Thermus thermophilus HB27	39.155	92.87	0.364