Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   IXK98_RS10865 Genome accession   NZ_CP064858
Coordinates   459469..459903 (-) Length   144 a.a.
NCBI ID   WP_069667590.1    Uniprot ID   A0A178JAH3
Organism   Vibrio europaeus strain 07/118 T2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 454469..464903
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IXK98_RS10845 (IXK98_10845) coaE 455001..455609 (-) 609 WP_069667594.1 dephospho-CoA kinase -
  IXK98_RS10850 (IXK98_10850) pilD 455611..456480 (-) 870 WP_171801081.1 prepilin peptidase Machinery gene
  IXK98_RS10855 (IXK98_10855) pilC 456538..457761 (-) 1224 WP_171801082.1 type II secretion system F family protein Machinery gene
  IXK98_RS10860 (IXK98_10860) pilB 457772..459463 (-) 1692 WP_171801083.1 type IV-A pilus assembly ATPase PilB Machinery gene
  IXK98_RS10865 (IXK98_10865) pilA 459469..459903 (-) 435 WP_069667590.1 type IV pilin protein Machinery gene
  IXK98_RS10870 (IXK98_10870) nadC 460146..461033 (-) 888 WP_195877354.1 carboxylating nicotinate-nucleotide diphosphorylase -
  IXK98_RS10875 (IXK98_10875) ampD 461165..461719 (+) 555 WP_171801084.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  IXK98_RS10880 (IXK98_10880) fldB 461957..462478 (-) 522 WP_171801085.1 flavodoxin FldB -
  IXK98_RS10885 (IXK98_10885) xerD 462641..463537 (+) 897 WP_171802358.1 site-specific tyrosine recombinase XerD -
  IXK98_RS10890 (IXK98_10890) - 463578..464330 (+) 753 WP_171382778.1 thioredoxin fold domain-containing protein -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 14567.37 Da        Isoelectric Point: 4.4295

>NTDB_id=505383 IXK98_RS10865 WP_069667590.1 459469..459903(-) (pilA) [Vibrio europaeus strain 07/118 T2]
MQSSQSSKAKGFTLIELMIVVAIIGVLASISIPAYQDYVRKSEAASALATLKSLITPAEVFYQENGTASAAALSNLGIDS
GANNLGTIASAITGTGSAAVPTLKFTFDSDSSMGSSDTITYSRDPSTGWSCAKAGAVPSLDGCS

Nucleotide


Download         Length: 435 bp        

>NTDB_id=505383 IXK98_RS10865 WP_069667590.1 459469..459903(-) (pilA) [Vibrio europaeus strain 07/118 T2]
ATGCAAAGTTCACAAAGCAGCAAAGCCAAAGGCTTTACCTTAATTGAGTTGATGATTGTGGTAGCAATTATTGGCGTGCT
TGCGTCGATATCAATACCAGCTTATCAAGACTATGTTAGGAAATCTGAGGCGGCTTCAGCGCTAGCGACGTTAAAAAGCC
TGATTACGCCAGCTGAAGTTTTCTATCAAGAAAACGGCACCGCATCGGCCGCCGCTTTATCTAATTTAGGCATCGATAGC
GGCGCTAATAATTTAGGAACCATTGCCTCAGCAATAACTGGAACAGGTTCAGCAGCTGTTCCAACATTAAAGTTCACATT
CGATTCCGACAGCTCAATGGGAAGCAGTGACACCATTACCTATTCTCGAGACCCGAGTACCGGTTGGTCTTGCGCAAAAG
CGGGAGCAGTCCCTTCTCTAGATGGATGCTCATAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(41-134)

Prokaryotic N-terminal methylation motif (N_methyl)

(7-31)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A178JAH3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Vibrio parahaemolyticus RIMD 2210633

59.028

100

0.59
  pilA Pseudomonas aeruginosa PAK

44.275

90.972

0.403
  pilA Vibrio cholerae strain A1552

40.58

95.833

0.389
  pilA Vibrio cholerae C6706

40.58

95.833

0.389
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

40.58

95.833

0.389
  comP Acinetobacter baylyi ADP1

38.406

95.833

0.368
  pilA/pilA1 Eikenella corrodens VA1

38.519

93.75

0.361