Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   IXK98_RS10860 Genome accession   NZ_CP064858
Coordinates   457772..459463 (-) Length   563 a.a.
NCBI ID   WP_171801083.1    Uniprot ID   A0AAE7DVZ4
Organism   Vibrio europaeus strain 07/118 T2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 452772..464463
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IXK98_RS10830 (IXK98_10830) rplS 453293..453646 (+) 354 WP_004742596.1 50S ribosomal protein L19 -
  IXK98_RS10835 (IXK98_10835) yacG 453969..454163 (-) 195 WP_069667595.1 DNA gyrase inhibitor YacG -
  IXK98_RS10840 (IXK98_10840) zapD 454226..454966 (-) 741 WP_004742598.1 cell division protein ZapD -
  IXK98_RS10845 (IXK98_10845) coaE 455001..455609 (-) 609 WP_069667594.1 dephospho-CoA kinase -
  IXK98_RS10850 (IXK98_10850) pilD 455611..456480 (-) 870 WP_171801081.1 prepilin peptidase Machinery gene
  IXK98_RS10855 (IXK98_10855) pilC 456538..457761 (-) 1224 WP_171801082.1 type II secretion system F family protein Machinery gene
  IXK98_RS10860 (IXK98_10860) pilB 457772..459463 (-) 1692 WP_171801083.1 type IV-A pilus assembly ATPase PilB Machinery gene
  IXK98_RS10865 (IXK98_10865) pilA 459469..459903 (-) 435 WP_069667590.1 type IV pilin protein Machinery gene
  IXK98_RS10870 (IXK98_10870) nadC 460146..461033 (-) 888 WP_195877354.1 carboxylating nicotinate-nucleotide diphosphorylase -
  IXK98_RS10875 (IXK98_10875) ampD 461165..461719 (+) 555 WP_171801084.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  IXK98_RS10880 (IXK98_10880) fldB 461957..462478 (-) 522 WP_171801085.1 flavodoxin FldB -
  IXK98_RS10885 (IXK98_10885) xerD 462641..463537 (+) 897 WP_171802358.1 site-specific tyrosine recombinase XerD -
  IXK98_RS10890 (IXK98_10890) - 463578..464330 (+) 753 WP_171382778.1 thioredoxin fold domain-containing protein -

Sequence

Protein


Download         Length: 563 a.a.        Molecular weight: 62507.44 Da        Isoelectric Point: 5.3739

>NTDB_id=505382 IXK98_RS10860 WP_171801083.1 457772..459463(-) (pilB) [Vibrio europaeus strain 07/118 T2]
MLTNLPSILRQAELLDPVQEAQLVKLVKDTDLTVAEVIIEQGWLTQAELASQLSRIFALPIIDFANYDYQTVCHNLGLRE
LITRSRAVPIRQSEHQLLIAVTDPTNIAIEEEFRFATGKQIELVLADIKQLNGAVRRLYGSAICGEEASHAKELSEQELV
SLVELSNDEINPVEDLSSDHSPVSRYINQILLDAVRKQASDIHFEPYEASYRVRFRCDGILIETQQPSHTLSRRLSARLK
IIAKLDIAERRLPQDGRIKLQLSASTAVDIRISTLPTLWGEKIVLRLLDSSTAELNIQSLGYNQHQQALYQKALSKPQGM
ILMTGPTGSGKTVSLYAGLNMLNTPQVNIATAEDPVEINLAGVNQVQINPKIGLDFAQALRAFLRQDPDIVMVGEIRDFE
TAEIAVKAAQTGHLVLSTLHTNSAAESVVRLQHMGISPFNVSSSLNLIIAQRLARKLCNHCKQQQTLPETHCQQLELANN
TVVFAANEQGCSECNQGYFGRTGIYEVMQINDELVHVINQEADAKAIENVAIKQGMQTLKESGTEKLLAGVTSYQELQRV
LYL

Nucleotide


Download         Length: 1692 bp        

>NTDB_id=505382 IXK98_RS10860 WP_171801083.1 457772..459463(-) (pilB) [Vibrio europaeus strain 07/118 T2]
ATGCTAACCAACCTACCCTCTATTTTGCGCCAAGCTGAGCTTCTTGACCCTGTTCAAGAAGCTCAGTTAGTTAAGTTGGT
TAAAGATACTGACCTTACCGTAGCCGAAGTCATAATAGAGCAAGGTTGGCTCACCCAAGCGGAGCTAGCCTCACAACTCT
CGCGAATTTTTGCCTTACCCATCATCGACTTTGCAAATTACGATTATCAAACGGTATGCCACAACTTAGGCCTGCGTGAA
CTGATCACCCGCTCCCGAGCAGTACCTATTAGGCAGAGTGAACACCAACTGCTTATCGCCGTTACCGACCCAACCAATAT
CGCAATCGAAGAAGAGTTTCGCTTTGCAACCGGAAAGCAAATTGAGCTAGTTTTAGCTGACATAAAGCAGCTCAATGGCG
CGGTACGTCGCTTGTATGGCAGCGCTATCTGTGGTGAAGAAGCGAGTCATGCAAAAGAGCTGAGCGAACAAGAGCTCGTC
AGCCTAGTCGAGCTCTCTAATGATGAGATTAACCCAGTCGAAGACCTGAGTAGTGATCACTCACCAGTCAGCCGTTATAT
CAATCAAATCTTATTAGATGCGGTTAGGAAGCAAGCTTCTGATATCCACTTTGAACCCTATGAAGCCTCTTATCGTGTGC
GCTTTCGCTGTGATGGCATTTTGATTGAGACTCAGCAACCTAGCCATACATTAAGTCGCCGTTTATCCGCAAGACTAAAG
ATCATCGCCAAACTCGATATTGCCGAGCGCCGCTTACCTCAAGATGGGCGCATCAAGCTTCAACTAAGTGCAAGTACCGC
TGTCGATATTCGTATTTCAACCCTACCAACTCTATGGGGCGAGAAGATCGTGCTCCGTCTTCTTGATAGCAGCACTGCCG
AGTTAAACATTCAATCACTAGGGTACAACCAACACCAACAAGCTTTGTATCAAAAGGCTTTGAGCAAGCCCCAAGGCATG
ATTTTGATGACAGGACCAACAGGCAGCGGTAAGACAGTCTCACTTTATGCGGGGTTAAACATGCTCAATACCCCACAAGT
GAACATCGCCACCGCAGAAGACCCGGTGGAAATTAATTTAGCGGGCGTTAACCAAGTTCAAATCAACCCTAAAATTGGAT
TAGACTTCGCCCAAGCGTTACGCGCCTTCCTACGCCAAGACCCGGACATTGTCATGGTTGGTGAGATACGCGACTTCGAA
ACTGCTGAAATCGCAGTCAAAGCGGCACAAACAGGTCACCTAGTGCTATCAACATTGCATACGAACTCGGCTGCCGAAAG
CGTCGTGCGCTTGCAACATATGGGCATCTCCCCATTCAATGTCTCTTCATCATTGAACTTAATCATTGCCCAGCGTTTGG
CACGAAAGCTGTGTAACCATTGTAAGCAGCAGCAAACCTTGCCCGAGACACATTGCCAACAACTGGAGTTAGCAAACAAC
ACCGTGGTTTTTGCTGCTAATGAGCAAGGGTGCAGTGAATGCAATCAAGGCTACTTTGGGCGAACCGGAATCTATGAAGT
GATGCAGATAAACGATGAGTTAGTGCACGTCATCAATCAAGAGGCAGATGCTAAAGCCATCGAAAACGTCGCGATAAAGC
AAGGTATGCAGACGCTTAAAGAATCTGGAACGGAGAAACTGCTCGCAGGAGTGACCAGCTACCAAGAGTTGCAACGTGTT
CTTTACTTGTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(39-141)

Type II/IV secretion system protein (T2SSE)

(189-456)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

64.121

100

0.641
  pilB Vibrio parahaemolyticus RIMD 2210633

61.837

100

0.622
  pilB Vibrio campbellii strain DS40M4

61.279

100

0.613
  pilB Acinetobacter baumannii D1279779

48.689

94.849

0.462
  pilB Legionella pneumophila strain ERS1305867

46.237

99.112

0.458
  pilB Acinetobacter baylyi ADP1

47.269

94.316

0.446
  pilF Neisseria gonorrhoeae MS11

44.361

94.494

0.419
  pilB Haemophilus influenzae 86-028NP

42.827

85.435

0.366
  pilB Haemophilus influenzae Rd KW20

42.268

86.146

0.364
  pilF Thermus thermophilus HB27

38.06

95.204

0.362