Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   IS519_RS11800 Genome accession   NZ_CP064170
Coordinates   2631420..2633138 (+) Length   572 a.a.
NCBI ID   WP_060983392.1    Uniprot ID   -
Organism   Vibrio crassostreae strain ED395     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2626420..2638138
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IS519_RS11780 (IS519_11770) pilM 2628649..2629674 (+) 1026 WP_248638937.1 type IV pilus assembly protein PilM Machinery gene
  IS519_RS11785 (IS519_11775) pilN 2629658..2630257 (+) 600 WP_248638939.1 PilN domain-containing protein Machinery gene
  IS519_RS11790 (IS519_11780) pilO 2630250..2630879 (+) 630 WP_248638941.1 type 4a pilus biogenesis protein PilO Machinery gene
  IS519_RS11795 (IS519_11785) pilP 2630866..2631384 (+) 519 WP_060983393.1 pilus assembly protein PilP Machinery gene
  IS519_RS11800 (IS519_11790) pilQ 2631420..2633138 (+) 1719 WP_060983392.1 type IV pilus secretin PilQ family protein Machinery gene
  IS519_RS11805 (IS519_11795) aroK 2633315..2633833 (+) 519 WP_017061125.1 shikimate kinase AroK -
  IS519_RS11810 (IS519_11800) aroB 2633859..2634947 (+) 1089 WP_017061124.1 3-dehydroquinate synthase -
  IS519_RS11815 (IS519_11805) - 2635027..2636535 (+) 1509 WP_248638943.1 AAA family ATPase -
  IS519_RS11820 (IS519_11810) - 2636605..2637453 (+) 849 WP_248638945.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -

Sequence

Protein


Download         Length: 572 a.a.        Molecular weight: 62049.75 Da        Isoelectric Point: 4.3846

>NTDB_id=499943 IS519_RS11800 WP_060983392.1 2631420..2633138(+) (pilQ) [Vibrio crassostreae strain ED395]
MIKGISGLVSKALQGFAVSVMLVVSVLSHAESLPNKLENIDFRVNKDKDAVIIIELATSTAVVDVQRAQEGLSIELINTQ
VDDDKLYLLDVKDFATLVEGIEVYKETPSTRLLATISNDYQYEYNLKGRFIEVVISKPVIDDATKEKSVLEKEGKLISIN
FQDIPVRNVLQLIADYNDFNLVVSDSVAGNLTLRLDGVPWQQVLDIILQVKGLDKRVDGNVILVAPKAELDLREQQALEK
SRLEEELGELKSEIIKINFAKATDIADMIGGEGAVSMLSDRGSITIDERTNSLLIRELEENIAVIRGIIESLDIPVKQVQ
IEARIVTVTEGNLDELGVRWGVSSTNGSFTVGGSIEGNHPSTITPYDDDSGSSVIDDYLNVNLGATSPNASSIAFQVAKL
GSDTLLDLELSALQQESKAEIISSPRLITTNKKPAYIEQGTEIPYLESSSSGATSVAFKKAVLSLKVTPQITPDNRLVLD
LSVTQDRPGQVVKTGTGEAVAIDTQRIGTQVLVNNGETVVLGGIFQHSVSSTVDKVPLLGDLPVLGALFRRSYENVGKSE
LLIFVTPKVVIQ

Nucleotide


Download         Length: 1719 bp        

>NTDB_id=499943 IS519_RS11800 WP_060983392.1 2631420..2633138(+) (pilQ) [Vibrio crassostreae strain ED395]
ATGATTAAAGGAATAAGTGGATTAGTGAGCAAGGCTCTACAAGGTTTTGCTGTGTCTGTGATGTTGGTTGTTAGCGTGCT
CAGCCATGCCGAGAGCTTACCGAACAAATTGGAGAATATTGATTTTAGGGTCAATAAGGATAAAGACGCCGTCATCATTA
TTGAGCTGGCGACCAGCACTGCTGTGGTGGATGTTCAACGTGCTCAGGAAGGATTAAGTATCGAGTTAATCAATACTCAG
GTTGATGATGACAAGCTTTACCTACTAGATGTAAAAGATTTCGCTACCTTGGTTGAAGGCATTGAAGTCTATAAAGAGAC
ACCAAGCACTCGGCTTCTAGCGACGATTAGCAATGACTATCAGTATGAATACAATCTAAAGGGACGTTTTATTGAGGTGG
TGATCAGCAAACCGGTTATCGATGACGCGACCAAAGAAAAAAGCGTTCTAGAGAAAGAGGGCAAGCTGATATCGATTAAC
TTCCAGGATATCCCAGTACGAAATGTCCTGCAGCTGATTGCCGACTACAACGACTTCAATCTCGTGGTGTCTGATTCGGT
AGCCGGAAACCTGACGTTACGCTTAGATGGCGTACCTTGGCAGCAAGTTCTCGACATTATCTTGCAAGTTAAAGGCTTGG
ATAAGCGTGTTGATGGCAATGTTATCTTGGTCGCGCCGAAAGCGGAGCTCGACCTTCGAGAGCAACAAGCGTTAGAAAAA
TCTCGCTTAGAAGAGGAGTTAGGGGAGCTTAAATCGGAAATCATCAAGATTAATTTTGCAAAAGCCACCGACATTGCTGA
CATGATTGGCGGTGAAGGTGCGGTGAGCATGTTGTCCGATCGCGGCTCAATTACCATTGATGAGAGAACCAACTCACTGC
TCATTCGTGAATTGGAAGAAAACATTGCCGTGATCCGAGGCATCATTGAATCGCTAGATATTCCCGTGAAGCAAGTGCAG
ATAGAGGCACGTATTGTCACGGTTACCGAGGGTAACCTAGATGAGCTTGGCGTGCGCTGGGGCGTTTCTTCAACCAACGG
TAGCTTCACCGTTGGCGGTTCAATCGAAGGTAACCACCCATCAACGATTACGCCGTATGACGACGATAGCGGCAGCAGCG
TGATTGATGACTATCTCAACGTCAATTTAGGGGCAACATCGCCGAACGCCTCAAGTATTGCATTTCAGGTGGCCAAGCTG
GGCTCAGATACCTTGCTTGATCTTGAACTGTCGGCACTGCAACAAGAGTCGAAAGCTGAAATCATTTCCAGCCCACGCTT
AATCACCACCAATAAAAAGCCCGCTTATATTGAGCAAGGTACTGAAATTCCTTACTTAGAGTCATCTTCAAGTGGCGCTA
CGTCGGTTGCGTTTAAGAAAGCGGTATTGAGTCTTAAGGTGACACCGCAGATCACACCCGACAATCGCTTGGTATTGGAT
TTGAGCGTGACTCAAGATAGGCCAGGGCAAGTGGTAAAAACCGGAACAGGTGAAGCGGTCGCAATTGATACGCAAAGAAT
AGGAACGCAAGTGCTTGTTAATAATGGTGAAACTGTCGTGCTTGGCGGTATATTTCAGCACAGCGTGAGCAGTACAGTGG
ATAAAGTTCCTCTGTTGGGAGACCTGCCAGTTTTGGGTGCATTGTTCCGTCGCAGCTATGAAAATGTGGGTAAAAGTGAA
CTACTTATTTTTGTCACACCCAAAGTTGTGATTCAGTAA

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(412-570)

Bacterial type II/III secretion system short domain (Secretin_N)

(253-318)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Vibrio cholerae strain A1552

77.352

100

0.776
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

77.352

100

0.776
  pilQ Vibrio campbellii strain DS40M4

81.066

95.105

0.771
  pilQ Legionella pneumophila strain Lp02

42.857

95.455

0.409
  pilQ Legionella pneumophila strain ERS1305867

42.857

95.455

0.409
  pilQ Pseudomonas aeruginosa PAK

40.893

97.902

0.4
  comQ Acinetobacter baylyi ADP1

38.957

100

0.392
  pilQ Acinetobacter baumannii strain A118

37.651

100

0.381
  pilQ Acinetobacter baumannii D1279779

37.651

100

0.381