Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   IS519_RS11780 Genome accession   NZ_CP064170
Coordinates   2628649..2629674 (+) Length   341 a.a.
NCBI ID   WP_248638937.1    Uniprot ID   -
Organism   Vibrio crassostreae strain ED395     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2623649..2634674
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IS519_RS11760 (IS519_11755) - 2623979..2624707 (-) 729 WP_017061133.1 glutathione peroxidase -
  IS519_RS11765 - 2624752..2624932 (-) 181 Protein_2237 hypothetical protein -
  IS519_RS11770 (IS519_11760) oxyR 2624931..2625824 (+) 894 WP_017061132.1 DNA-binding transcriptional regulator OxyR -
  IS519_RS11775 (IS519_11765) - 2625936..2628497 (-) 2562 WP_248638935.1 PBP1A family penicillin-binding protein -
  IS519_RS11780 (IS519_11770) pilM 2628649..2629674 (+) 1026 WP_248638937.1 type IV pilus assembly protein PilM Machinery gene
  IS519_RS11785 (IS519_11775) pilN 2629658..2630257 (+) 600 WP_248638939.1 PilN domain-containing protein Machinery gene
  IS519_RS11790 (IS519_11780) pilO 2630250..2630879 (+) 630 WP_248638941.1 type 4a pilus biogenesis protein PilO Machinery gene
  IS519_RS11795 (IS519_11785) pilP 2630866..2631384 (+) 519 WP_060983393.1 pilus assembly protein PilP Machinery gene
  IS519_RS11800 (IS519_11790) pilQ 2631420..2633138 (+) 1719 WP_060983392.1 type IV pilus secretin PilQ family protein Machinery gene
  IS519_RS11805 (IS519_11795) aroK 2633315..2633833 (+) 519 WP_017061125.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 341 a.a.        Molecular weight: 38299.68 Da        Isoelectric Point: 6.4182

>NTDB_id=499939 IS519_RS11780 WP_248638937.1 2628649..2629674(+) (pilM) [Vibrio crassostreae strain ED395]
MGSSLITGIDINHHSIKAVVLKPVGELYALVGYKELPISDDIFTANHTLEYQKTVKKLKELRKGLPFGCRNVAISVPDNT
VNSKVLQIESELEDREKEFSIYQTFAHQSPFPIEELSLDFVKLEDKRFGKGSTSSYQVYATRKEVVDSRADALTKAGFKP
VVVDTQAHGLLNIWQLASRLYPDKKNWLLVDVGIDQTSLGVIPQNSAPFYKDIAFGTQDLRSSDDPNDIESVFGTMEETH
QFIVNLIEKLKRQLQLYSSGNAQQPISGIWLMGEGASIPMVTEELERHFQLSCESLNPLSLFENKVAKRRRLPMDWQHFG
IAAGMAMSGLKWQGEKHVASN

Nucleotide


Download         Length: 1026 bp        

>NTDB_id=499939 IS519_RS11780 WP_248638937.1 2628649..2629674(+) (pilM) [Vibrio crassostreae strain ED395]
ATGGGTTCATCATTAATTACAGGTATAGATATAAATCATCACAGCATCAAAGCCGTGGTACTAAAACCCGTGGGGGAGTT
GTATGCCCTAGTGGGATACAAAGAGCTGCCGATTTCGGACGACATTTTTACAGCTAACCATACTTTGGAGTATCAGAAAA
CTGTTAAGAAACTTAAAGAACTTAGGAAAGGACTGCCTTTTGGCTGTCGCAACGTCGCCATTTCGGTACCTGATAACACA
GTGAACAGCAAAGTACTGCAAATAGAGAGTGAGTTAGAAGACAGAGAGAAAGAGTTTTCTATCTATCAAACCTTCGCCCA
TCAATCTCCCTTTCCTATCGAGGAGCTGAGTTTAGATTTTGTAAAGCTGGAAGACAAACGATTTGGTAAAGGTTCGACAA
GCAGTTATCAGGTATACGCCACTCGTAAGGAAGTGGTGGATAGCCGAGCGGATGCATTAACTAAGGCTGGCTTTAAACCT
GTGGTGGTGGATACGCAGGCGCATGGGCTACTTAATATCTGGCAATTGGCCTCGCGTCTGTATCCTGATAAGAAAAACTG
GTTGCTGGTGGATGTTGGAATCGACCAAACCTCGCTAGGGGTTATCCCGCAGAATTCGGCGCCTTTCTATAAAGACATCG
CCTTTGGCACTCAAGATCTTCGCAGCTCAGATGACCCGAATGATATCGAGAGCGTGTTCGGTACTATGGAAGAGACACAT
CAATTCATCGTTAACCTGATTGAGAAGCTCAAGCGTCAATTGCAGCTTTATTCATCGGGAAATGCGCAGCAACCTATTTC
TGGGATTTGGTTGATGGGCGAGGGCGCCAGCATTCCTATGGTAACCGAAGAGCTAGAACGTCATTTTCAGCTGAGTTGCG
AGTCATTGAACCCTTTGTCTCTGTTTGAGAATAAGGTCGCCAAGAGACGCCGACTGCCGATGGACTGGCAACACTTTGGT
ATCGCGGCGGGTATGGCGATGAGTGGACTCAAGTGGCAGGGAGAGAAGCATGTTGCATCAAATTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-328)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio cholerae strain A1552

54.252

100

0.543
  pilM Vibrio campbellii strain DS40M4

48.397

100

0.487