Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   IS519_RS11790 Genome accession   NZ_CP064170
Coordinates   2630250..2630879 (+) Length   209 a.a.
NCBI ID   WP_248638941.1    Uniprot ID   -
Organism   Vibrio crassostreae strain ED395     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2625250..2635879
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IS519_RS11775 (IS519_11765) - 2625936..2628497 (-) 2562 WP_248638935.1 PBP1A family penicillin-binding protein -
  IS519_RS11780 (IS519_11770) pilM 2628649..2629674 (+) 1026 WP_248638937.1 type IV pilus assembly protein PilM Machinery gene
  IS519_RS11785 (IS519_11775) pilN 2629658..2630257 (+) 600 WP_248638939.1 PilN domain-containing protein Machinery gene
  IS519_RS11790 (IS519_11780) pilO 2630250..2630879 (+) 630 WP_248638941.1 type 4a pilus biogenesis protein PilO Machinery gene
  IS519_RS11795 (IS519_11785) pilP 2630866..2631384 (+) 519 WP_060983393.1 pilus assembly protein PilP Machinery gene
  IS519_RS11800 (IS519_11790) pilQ 2631420..2633138 (+) 1719 WP_060983392.1 type IV pilus secretin PilQ family protein Machinery gene
  IS519_RS11805 (IS519_11795) aroK 2633315..2633833 (+) 519 WP_017061125.1 shikimate kinase AroK -
  IS519_RS11810 (IS519_11800) aroB 2633859..2634947 (+) 1089 WP_017061124.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 209 a.a.        Molecular weight: 24332.82 Da        Isoelectric Point: 4.5843

>NTDB_id=499941 IS519_RS11790 WP_248638941.1 2630250..2630879(+) (pilO) [Vibrio crassostreae strain ED395]
MASFQNRISWQNRMSLQDLDVDEITEWPLLPQLLVILVLIVLIQGVGTWLYVLPLDEELQQMKQQEQTLKTTLRIKANKV
AALPKLQSQLDELTSRYDYLLEQLPVQKELASMLASVNELGLDNSLTFTRIDWGQKQNKEFLYRLPLNIELTGDYHEIGD
FSAAIAKLPRIISFDDVNWQRVSQESSTLHFRVRAYTYQFKSEVNDETQ

Nucleotide


Download         Length: 630 bp        

>NTDB_id=499941 IS519_RS11790 WP_248638941.1 2630250..2630879(+) (pilO) [Vibrio crassostreae strain ED395]
ATGGCTAGTTTTCAAAATAGGATTAGTTGGCAAAACAGGATGAGCTTGCAAGATCTCGATGTTGATGAGATCACCGAATG
GCCATTGTTGCCTCAGCTTCTTGTGATTCTGGTGTTGATAGTGTTAATCCAAGGTGTCGGGACTTGGCTTTATGTGCTGC
CGCTCGACGAGGAACTGCAACAGATGAAGCAACAGGAGCAGACCTTAAAAACGACCTTGAGAATAAAAGCCAACAAGGTT
GCAGCCTTACCTAAGTTGCAGAGCCAGTTGGATGAGCTAACTAGTCGCTACGATTATCTATTAGAGCAGCTGCCCGTTCA
AAAGGAGTTAGCGAGCATGCTGGCCTCCGTGAATGAGCTCGGCTTGGATAACTCGCTGACCTTCACACGAATCGATTGGG
GGCAAAAGCAGAATAAGGAGTTTCTCTATCGATTGCCTCTCAATATAGAGCTGACAGGTGACTATCACGAGATTGGGGAC
TTCTCTGCTGCTATCGCCAAGCTGCCGCGCATCATCAGCTTTGATGATGTGAATTGGCAGCGAGTCAGCCAAGAAAGTAG
CACGCTGCATTTTAGGGTTCGAGCTTATACCTACCAGTTTAAATCGGAGGTCAATGATGAAACCCAATAG

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(59-201)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Vibrio cholerae strain A1552

75.648

92.344

0.699
  pilO Vibrio campbellii strain DS40M4

72.973

88.517

0.646