Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   KE3_RS00235 Genome accession   NC_021900
Coordinates   47941..48882 (+) Length   313 a.a.
NCBI ID   WP_020915987.1    Uniprot ID   A0AB33AJ80
Organism   Streptococcus lutetiensis 033     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 42941..53882
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KE3_RS00225 (KE3_0047) rpoC 43706..47344 (+) 3639 WP_020915985.1 DNA-directed RNA polymerase subunit beta' -
  KE3_RS00230 (KE3_0048) - 47501..47869 (+) 369 WP_020915986.1 DUF1033 family protein -
  KE3_RS00235 (KE3_0049) comGA 47941..48882 (+) 942 WP_020915987.1 competence type IV pilus ATPase ComGA Machinery gene
  KE3_RS00240 (KE3_0050) comGB 48806..49849 (+) 1044 WP_020915988.1 competence type IV pilus assembly protein ComGB Machinery gene
  KE3_RS00245 (KE3_0051) comGC 49849..50142 (+) 294 WP_020915989.1 competence type IV pilus major pilin ComGC Machinery gene
  KE3_RS00250 (KE3_0052) comGD 50126..50557 (+) 432 WP_020915990.1 competence type IV pilus minor pilin ComGD Machinery gene
  KE3_RS00255 (KE3_0053) comGE 50511..50804 (+) 294 WP_020915991.1 competence type IV pilus minor pilin ComGE Machinery gene
  KE3_RS00260 (KE3_0054) comGF 50788..51225 (+) 438 WP_043894883.1 competence type IV pilus minor pilin ComGF Machinery gene
  KE3_RS00265 (KE3_0055) comGG 51197..51547 (+) 351 WP_020915993.1 competence type IV pilus minor pilin ComGG Machinery gene
  KE3_RS00270 (KE3_0056) comGH 51601..52557 (+) 957 WP_020915994.1 class I SAM-dependent methyltransferase Machinery gene
  KE3_RS00275 (KE3_0057) - 52611..53810 (+) 1200 WP_020915995.1 acetate kinase -

Sequence

Protein


Download         Length: 313 a.a.        Molecular weight: 35692.88 Da        Isoelectric Point: 5.9480

>NTDB_id=49991 KE3_RS00235 WP_020915987.1 47941..48882(+) (comGA) [Streptococcus lutetiensis 033]
MIQEKAKQLIKDAVEKNVQDIYIVPKKEHYEIYQRVGDERQFIQEVASDEMTSVISHFKFVAGMNVGEKRRSQLGSCDYE
FAEGEEISLRLSTVGDYRGRESLVIRLLYSGRHDLQYWFDGMKNILEAIDSRGLYLFSGPVGSGKTTLMYQLVREKFPDK
QVITIEDPVEIKQDNMLQLQLNDEIGMTYDNLIKLSLRHRPDILIIGEIRDSETARAVIRASLTGVLVFSTIHAKSIPGV
YARLLELGVSRQELENSLRAIIYQRLIGGGGIVDFAKGDFEHYPPTRWNEQIKSLARDGHIGLEEAKIEKIAL

Nucleotide


Download         Length: 942 bp        

>NTDB_id=49991 KE3_RS00235 WP_020915987.1 47941..48882(+) (comGA) [Streptococcus lutetiensis 033]
ATGATTCAGGAAAAAGCAAAGCAGCTGATTAAGGATGCTGTTGAAAAGAATGTGCAAGATATTTACATTGTTCCTAAGAA
GGAGCACTATGAAATTTATCAACGTGTTGGAGATGAACGTCAGTTTATCCAAGAAGTTGCTAGTGATGAAATGACAAGCG
TGATTAGTCACTTTAAATTTGTTGCGGGAATGAATGTTGGTGAGAAACGTCGTAGTCAGCTAGGGTCTTGTGATTATGAA
TTTGCTGAGGGTGAAGAGATTTCACTGCGTTTATCCACTGTTGGTGATTATCGCGGGCGTGAGAGCCTGGTCATCAGACT
TCTTTATTCAGGTCGTCATGATTTGCAATATTGGTTCGATGGGATGAAGAATATTTTAGAGGCGATTGATAGTCGAGGCC
TTTATCTTTTTTCCGGGCCGGTAGGGAGTGGAAAGACTACTCTCATGTATCAATTGGTTCGTGAAAAGTTCCCCGATAAG
CAGGTCATTACGATAGAAGACCCTGTTGAGATTAAGCAGGATAATATGTTGCAGCTGCAGCTTAATGATGAAATTGGCAT
GACTTATGATAATCTCATTAAACTCTCGCTTCGCCATAGACCTGATATTTTAATTATTGGAGAGATTCGTGATAGTGAGA
CAGCGCGTGCGGTTATTAGAGCTAGTCTAACAGGTGTGCTCGTCTTTTCAACTATTCATGCGAAAAGTATCCCAGGTGTT
TATGCCAGATTATTGGAGTTGGGAGTTAGTAGACAAGAGCTCGAAAATAGTTTACGAGCGATTATTTATCAACGATTAAT
TGGCGGTGGAGGTATTGTGGATTTTGCCAAAGGAGATTTTGAACACTATCCCCCAACAAGGTGGAATGAGCAAATTAAAA
GCCTTGCTAGGGACGGACATATTGGCCTTGAGGAAGCAAAAATCGAAAAAATTGCCCTTTAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(8-267)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0AB33AJ80

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Streptococcus mutans UA159

71.061

99.361

0.706
  comGA Streptococcus mutans UA140

70.74

99.361

0.703
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

67.097

99.042

0.665
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

65.064

99.681

0.649
  comGA Streptococcus gordonii str. Challis substr. CH1

64.952

99.361

0.645
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

64.423

99.681

0.642
  comGA/cglA/cilD Streptococcus pneumoniae D39

64.423

99.681

0.642
  comGA/cglA/cilD Streptococcus pneumoniae R6

64.423

99.681

0.642
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

64.423

99.681

0.642
  comGA Lactococcus lactis subsp. cremoris KW2

53.994

100

0.54
  comGA Latilactobacillus sakei subsp. sakei 23K

43.066

87.54

0.377